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Home / Equine Research Bank / Arch Virol / Proviral sequences detected by polymerase chain reaction in ...
Archives of virology1991; 117(1-2); 109-119; doi: 10.1007/BF01310496

Proviral sequences detected by polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus.

Abstract: Proviral sequences in the peripheral blood mononuclear cells of 3 horses with acute equine infectious anemia virus were monitored using the polymerase chain reaction. Provirus was detected during the initial viremic episode in each horse and during each of 3 relapsing viremic cycles, although the appearance of provirus lagged behind the onset of viremia. Following each viremic episode, provirus levels in the peripheral monocytes decreased to less than 1 copy in 5 x 10(6) cells.
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Publication Date: 1991-01-01 PubMed ID: 1848747DOI: 10.1007/BF01310496Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines how proviral sequences in the blood cells of horses with Equine Infectious Anemia (EIA) virus can be tracked using the polymerase chain reaction (PCR) method. It specifically looks at the appearance and fluctuations of the provirus during different stages of the viral infection.

Monitoring of Proviral sequences with PCR

  • The researchers observed proviral sequences in the peripheral blood mononuclear cells of three horses inflicted with an acute Equine Infectious Anemia (EIA) virus. They used the polymerase chain reaction (PCR) method to closely monitor the presence of provirus (A form of a virus that has been integrated into the genetic material of a host cell) at various stages of EIA infection.

EIA Virus and Provirus Appearance

  • They discovered that the provirus was detected during the initial infection phase in each horse and during each of the three relapsing viremic cycles (times when the virus was present again in the bloodstream). However, there was a delay in the detection of provirus compared to the onset of viremia (presence of viruses in the bloodstream).

Fluctuation of Provirus Levels

  • The scientists also noted that the levels of detected provirus in the peripheral monocyte cells (a type of white blood cell) decreased significantly after each occurrence of viremia, with a reduction to less than one copy of provirus in 5 million cells.
  • This suggests that the provirus appears and then reduces its presence in the infected horse’s peripheral blood cells in a cyclical pattern along with the occurrences of the EIA virus in the bloodstream.

Significance of the Research

  • This study provides important insights into the behavior and tracking of proviral sequences in relation to EIA virus infection. Such understanding can potentially assist in diagnosis, treatment, and management of this disease in horses.
  • It also highlights the possible use of the PCR method as an effective tool to monitor the presence and fluctuations of provirus during different stages of viral infection, which could be applied to a broad range of diseases or conditions that involve viral infections.

Cite This Article

APA
O'Rourke KI, Besola ML, McGuire TC. (1991). Proviral sequences detected by polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus. Arch Virol, 117(1-2), 109-119. https://doi.org/10.1007/BF01310496

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 117
Issue: 1-2
Pages: 109-119

Researcher Affiliations

O'Rourke, K I
  • Department of Veterinary Microbiology and Pathology, Washington State University, Pullman.
Besola, M L
    McGuire, T C

      MeSH Terms

      • Animals
      • Base Sequence
      • DNA Probes
      • DNA, Viral / blood
      • Equine Infectious Anemia / blood
      • Equine Infectious Anemia / microbiology
      • Horses
      • Infectious Anemia Virus, Equine / genetics
      • Infectious Anemia Virus, Equine / isolation & purification
      • Leukocytes, Mononuclear / microbiology
      • Molecular Sequence Data
      • Polymerase Chain Reaction
      • Proviruses / genetics
      • Proviruses / isolation & purification
      • Sensitivity and Specificity

      Grant Funding

      • AI24166 / NIAID NIH HHS
      • AI24291 / NIAID NIH HHS

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      Citations

      This article has been cited 7 times.
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