Pulmonary epithelial lining fluid and plasma ascorbic acid concentrations in horses affected by recurrent airway obstruction.
Abstract: To determine the pulmonary epithelial lining fluid (ELF) concentrations and degree of oxidation of ascorbic acid in horses affected by recurrent airway obstruction (RAO) in the presence and absence of neutrophilic airway inflammation. Methods: 6 RAO-affected horses and 8 healthy control horses. Methods: Nonenzymatic antioxidant concentrations were determined in RBC, plasma, and ELF samples of control horses and RAO-affected horses in the presence and absence of airway inflammation. Results: ELF ascorbic acid concentration was decreased in RAO-affected horses with airway inflammation (median, 0.06 mmol/L; 25th and 75th percentiles, 0.0 and 0.4 mmol/L), compared with RAO-affected horses without airway inflammation (1.0 mmol/L; 0.7 and 1.5 mmol/L) and control horses (2.2 mmol/L; 1.4 and 2.2 mmol/L). Epithelial lining fluid ascorbic acid remained significantly lower in RAO-affected horses without airway inflammation than in control horses. Moreover, the ELF ascorbic acid redox ratio (ie, ratio of the concentrations of dehydroascorbate to total ascorbic acid) was higher in RAO-affected horses with airway inflammation (median, 0.85; 25th and 75th percentiles, 0.25 and 1.00), compared with RAO-affected horses without airway inflammation (0.04; 0.02 and 0.22). The number of neutrophils in bronchoalveolar lavage fluid was inversely related to the ELF ascorbic acid concentration (r = -0.81) and positively correlated with the ascorbic acid redox ratio (r = 0.65). Conclusions: Neutrophilic inflammation in horses affected by RAO is associated with a reduction in the ELF ascorbic acid pool. Nutritional supplementation with ascorbic acid derivatives in horses affected by RAO is an area for further investigation.
Publication Date: 2004-01-15 PubMed ID: 14719707DOI: 10.2460/ajvr.2004.65.80Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study aims to investigate the levels of ascorbic acid, also known as Vitamin C, in the pulmonary epithelial lining fluid (ELF) of horses affected by recurrent airway obstruction (RAO). Diminished ascorbic acid levels were found in the ELF of horses suffering from RAO, particularly those with airway inflammation. The researchers suggest that there might be a role for dietary supplements containing ascorbic acid derivatives for horses with this condition.
Research Methodology
- In this study, two groups of horses were considered: six horses with recurrent airway obstruction (RAO) and eight healthy control horses.
- Nonenzymatic antioxidant concentrations were determined in Red Blood Cells (RBC), plasma, and ELF samples collected from the horses.
Key Findings
- The concentration of ascorbic acid in the ELF was significantly lower in horses with RAO that had airway inflammation.
- Also, horses with RAO but without inflammation still showed lower levels of ELF ascorbic acid compared to the control group.
- Furthermore, the study found that the ascorbic acid redox ratio (ratio of the concentrations of dehydroascorbate to total ascorbic acid) was higher in RAO-affected horses with airway inflammation compared to those without inflammation.
- Finally, the study discovered a correlation between the level of neutrophils, a type of white blood cell, in bronchoalveolar lavage fluid and the concentration of ascorbic acid. High numbers of neutrophils indicated a lower level of ELF ascorbic acid and a higher ascorbic acid redox ratio.
Conclusions and Future Work
- The research concluded that inflammatory effects in horses suffering from RAO led to lower ascorbic acid levels in the ELF, complicating the condition.
- Therefore, the researchers indicate that nutritional supplementation with ascorbic acid derivatives might prove beneficial for horses affected by RAO, an area the researchers suggest merits further study.
Cite This Article
APA
Deaton CM, Marlin DJ, Smith NC, Harris PA, Roberts CA, Schroter RC, Kelly FJ.
(2004).
Pulmonary epithelial lining fluid and plasma ascorbic acid concentrations in horses affected by recurrent airway obstruction.
Am J Vet Res, 65(1), 80-87.
https://doi.org/10.2460/ajvr.2004.65.80 Publication
Researcher Affiliations
- Centre for Equine Studies, Animal Health Trust, Lanwades Park, Kentford, Suffolk, CB8 7UU, UK.
MeSH Terms
- Animals
- Antioxidants / analysis
- Ascorbic Acid / analysis
- Ascorbic Acid / blood
- Bronchoalveolar Lavage Fluid / chemistry
- Epithelium / physiopathology
- Horse Diseases / blood
- Horse Diseases / physiopathology
- Horses
- Lung / physiopathology
- Lung Diseases, Obstructive / blood
- Lung Diseases, Obstructive / physiopathology
- Lung Diseases, Obstructive / veterinary
- Pneumonia / physiopathology
- Pneumonia / veterinary
Citations
This article has been cited 4 times.- Luu Quoc Q, Cao Thi Bich T, Kim SH, Park HS, Shin YS. Administration of vitamin E attenuates airway inflammation through restoration of Nrf2 in a mouse model of asthma.. J Cell Mol Med 2021 Jul;25(14):6721-6732.
- Bullone M, Lavoie JP. The Contribution of Oxidative Stress and Inflamm-Aging in Human and Equine Asthma.. Int J Mol Sci 2017 Dec 5;18(12).
- Barton AK, Gehlen H. Pulmonary Remodeling in Equine Asthma: What Do We Know about Mediators of Inflammation in the Horse?. Mediators Inflamm 2016;2016:5693205.
- Khol-Parisini A, van den Hoven R, Leinker S, Hulan HW, Zentek J. Effects of feeding sunflower oil or seal blubber oil to horses with recurrent airway obstruction.. Can J Vet Res 2007 Jan;71(1):59-65.
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