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Archives of virology1976; 51(1-2); 107-114; doi: 10.1007/BF01317839

Purification and characterization of equine infectious anemia virus.

Abstract: EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.
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Publication Date: 1976-01-01 PubMed ID: 183628DOI: 10.1007/BF01317839Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on purifying and identifying the characteristics of the Equine Infectious Anemia (EIA) virus which causes a highly infectious and potentially deadly disease in horses.

Objective and Methodology

  • The researchers carried out the extraction of the EIA virus from cultures of equine fetal kidney cells. This process was followed using a substance called PEG (polyethylene glycol).
  • The purified EIA virus was then subjected to two rounds of sucrose-gradient sedimentation processes, which were between 5-30 per cent and 25-60 per cent respectively. This was a step where the virus was separated based on their densities.
  • An immunodiffusion test was used to monitor the process. This test helps to identify antigens (foreign substances that induce an immune response in the body) or antibodies (proteins produced by the immune system to neutralize these antigens) in a sample.

Findings

  • The findings showed that the purified EIA virus had a specific density of 1.162 (measured at 20 degrees Celsius) and a sedimentation constant of S20w=656. Both of these measures tell us more about the physical characteristics of the virus, and how it behaves in a fluid suspension.
  • Electron microscopy was used to visualize the virus. The researchers noticed that the virus particles were roughly 100 nanometers in diameter and had a variable but predominantly spherical shape. Microscopic visualisation provided detailed structural information about the virus.
  • They observed that the dense core (the part of the virus containing genetic material and other proteins) could be in various locations within the virus particle. This further enhances our understanding about the structural flexibility of the virus.

Significance

  • This research deepens our understanding of the EIA virus, its physical attributes, and behavior. It provides a clearer picture of the virus’ structure, which can aid in the development of treatments or vaccines.

Cite This Article

APA
Matheka HD, Coggins L, Shively JN, Norcross NL. (1976). Purification and characterization of equine infectious anemia virus. Arch Virol, 51(1-2), 107-114. https://doi.org/10.1007/BF01317839

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 51
Issue: 1-2
Pages: 107-114

Researcher Affiliations

Matheka, H D
    Coggins, L
      Shively, J N
        Norcross, N L

          MeSH Terms

          • Animals
          • Cell Membrane / microbiology
          • Centrifugation, Density Gradient
          • Chemical Precipitation
          • Culture Techniques
          • Horses
          • Inclusion Bodies, Viral
          • Infectious Anemia Virus, Equine / growth & development
          • Infectious Anemia Virus, Equine / isolation & purification
          • Infectious Anemia Virus, Equine / ultrastructure
          • Kidney / embryology
          • Leukocytes

          References

          This article includes 13 references
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          Citations

          This article has been cited 7 times.
          1. Sellon DC. Equine infectious anemia. Vet Clin North Am Equine Pract 1993 Aug;9(2):321-36.
            doi: 10.1016/s0749-0739(17)30399-1pubmed: 8395326google scholar: lookup
          2. Sellon DC, Fuller FJ, McGuire TC. The immunopathogenesis of equine infectious anemia virus. Virus Res 1994 May;32(2):111-38.
            doi: 10.1016/0168-1702(94)90038-8pubmed: 8067050google scholar: lookup
          3. Cheevers WP, Ackley CM, Crawford TB. Structural proteins of equine infectious anemia virus. J Virol 1978 Dec;28(3):997-1001.
            doi: 10.1128/JVI.28.3.997-1001.1978pubmed: 215790google scholar: lookup
          4. Rice NR, Simek S, Ryder OA, Coggins L. Detection of proviral DNA in horse cells infected with equine infectious anemia virus. J Virol 1978 Jun;26(3):577-83.
            doi: 10.1128/JVI.26.3.577-583.1978pubmed: 209211google scholar: lookup
          5. Weiland F, Matheka HD, Coggins L, Hatner D. Electron microscopic studies on equine infectious anemia virus (EIAV). Brief report. Arch Virol 1977;55(4):335-40.
            doi: 10.1007/BF01315055pubmed: 202230google scholar: lookup
          6. Cheevers WP, Archer BG, Crawford TB. Characterization of RNA from equine infectious anemia virus. J Virol 1977 Nov;24(2):489-97.
            doi: 10.1128/JVI.24.2.489-497.1977pubmed: 199735google scholar: lookup
          7. Archer BG, Crawford TB, McGuire TC, Frazier ME. RNA-dependent DNA polymerase associated with equine infectious anemia virus. J Virol 1977 Apr;22(1):16-22.
            doi: 10.1128/JVI.22.1.16-22.1977pubmed: 67219google scholar: lookup
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