Quantitation of immunoglobulin-bearing lymphocytes and lymphocyte response to mitogens in horses persistently infected by equine infectious anemia virus.
Abstract: A defect in lymphocyte function could be responsible for persistent infection by the equine infectious anemia virus. The number of lymphocytes bearing surface immunoglobulin, as detected by immunofluorescence, and lymphocyte response to mitogens were the same in uninfected and equine infectious anemia-infected animals. A defect in T or B lymphocyte numbers or ability to respond to stimuli was not detected in this chronic virus disease.
Publication Date: 1973-10-01 PubMed ID: 4582640PubMed Central: PMC422911DOI: 10.1128/iai.8.4.679-682.1973Google Scholar: Lookup
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- Journal Article
Summary
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The study investigates whether a deficiency in lymphocyte function could be responsible for a persistent infection by the equine infectious anemia virus in horses, and found no difference in terms of number of immunoglobulin-bearing lymphocytes or their response to mitogens between infected and uninfected animals.
Research Overview
- The research was performed in response to the observation of persistent infection by the equine infectious ania (EIA) virus in horses. The researchers were interested in whether a defect in lymphocyte function might be a contributing factor to this condition.
- The lymphocyte is a vital component of the immune system. It is a type of white blood cell that fights off viral infections, produces antibodies, and helps in the process of immunological memory.
- There are two main types of lymphocytes: B lymphocytes (B cells), which make antibodies that attack bacteria and toxins, and T lymphocytes (T cells), which attack body cells themselves when they have been taken over by viruses or have become cancerous.
Methodology
- The researchers quantified the number of lymphocytes that were bearing surface immunoglobulin, as identified by a process known as immunofluorescence. Immunoglobulin is an antibody, a protein produced by plasma cells (a type of B lymphocyte) in response to an antigen. This helps to neutralize pathogens like bacteria and viruses.
- They also assessed the lymphocyte response to mitogens — substances that stimulate cell division or mitosis. Mitogens can induce a lymphocyte to begin cell division, leading to the production of more lymphocytes and a stronger immune response.
Findings
- The results revealed that there was no difference between the infected and the uninfected horses in terms of immunoglobulin-bearing lymphocytes and the lymphocyte response to mitogens.
- These results ruled out the possibility of a defect in the number of T or B lymphocytes, or their ability to respond to stimuli as being a contributing factor to the persistent EIA infection.
- This finding suggested that, in this chronic viral disease, the number or functionality of lymphocytes is not compromised.
Cite This Article
APA
Banks KL, Henson JB.
(1973).
Quantitation of immunoglobulin-bearing lymphocytes and lymphocyte response to mitogens in horses persistently infected by equine infectious anemia virus.
Infect Immun, 8(4), 679-682.
https://doi.org/10.1128/iai.8.4.679-682.1973 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Equine Infectious Anemia / immunology
- Fluorescent Antibody Technique
- Goats / immunology
- Horses
- Immune Sera
- Immunoglobulins / analysis
- Lectins / pharmacology
- Lymphocyte Activation / drug effects
- Lymphocytes / immunology
- Mitogens / pharmacology
References
This article includes 15 references
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Citations
This article has been cited 5 times.- Sellon DC, Fuller FJ, McGuire TC. The immunopathogenesis of equine infectious anemia virus.. Virus Res 1994 May;32(2):111-38.
- Srivastava SK, Barnum DA. Lymphocyte stimulation response in horses against phytohaemagglutinin and M protein of Streptococcus equi using whole blood.. Can J Comp Med 1982 Jan;46(1):51-6.
- Shively MA, Banks KL, Greenlee A, Klevjer-Anderson P. Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia.. Infect Immun 1982 Apr;36(1):38-46.
- Valpotić I, Kastelan M, Rudolf M, Gerencer M, Jukić B, Basić I. T and B lymphocytes in horses persistently infected with equine infectious anaemia virus.. Vet Res Commun 1989;13(1):57-65.
- McGuire TC. Suppression of synthesis of an IgG subclass in a persistent viral infection.. Immunology 1976 Jan;30(1):17-24.
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