Quantitative characterization of lymphocyte populations in bronchoalveolar lavage fluid and peripheral blood of normal adult Arabian horses.
Abstract: Bronchoalveolar lavage fluid (BALF) and peripheral blood were obtained from each of 17 adult Arabian mares and absolute numbers and relative lymphocyte proportions were determined for total T lymphocytes, using CD2 as a marker, CD4 + T lymphocytes, CD8 + T lymphocytes, CD5 + lymphocytes, and sIgM + B lymphocytes. The marked variation in BALF cell recovery resulted in wide variation in absolute values for each lymphocyte subset. The relative proportions of gated BALF lymphocytes were much less variable and provided a basis for comparison of lymphocyte subsets between the BALF and peripheral blood in normal horses. The total lymphocyte population was comprised predominantly of T cells in both BALF (83 +/- 11% CD2 + lymphocytes) and peripheral blood (70 +/- 8% CD2 + lymphocytes). There was a significantly greater percentage of total T lymphocytes and a significantly lower percentage of B lymphocytes in BALF as compared with peripheral blood collected at the same time. The increase in total T lymphocytes in BALF reflected a significantly higher percentage of CD8 + T lymphocytes in BALF (39 +/- 7%) as compared with peripheral blood (18 +/- 5%). The mean percentages of CD4 + T lymphocytes (58 +/- 13% in BALF; 62 +/- 8% in peripheral blood) and CD5 + lymphocytes (78 +/- 11% in BALF; 71 +/- 7% in peripheral blood), a population composed primarily of T lymphocytes but also including a subset of sIgM + B lymphocytes, were not significantly different between the two compartments. These normal values in Arabian mares provide a basis for detection of disease associated changes in the lymphocyte populations and for determination of effects of age and breed on lymphocyte subpopulations in the BALF and peripheral blood.
Publication Date: 1996-05-01 PubMed ID: 8797274DOI: 10.1016/0165-2427(95)05510-xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This research studied the populations of different types of lymphocytes (a type of white blood cell) in the bronchoalveolar lavage fluid (BALF – a sample taken from the lungs) and peripheral blood of adult Arabian horses. It found that there was a higher percentage of T lymphocytes in BALF compared to peripheral blood, while the percentage of B lymphocytes was lower in BALF than in blood.
Sample Collection and Lymphocyte Counting
- The researchers collected BALF and peripheral blood samples from 17 adult Arabian mares and determined the number and proportion of different types of lymphocytes – total T lymphocytes (marked by CD2), CD4 and CD8 T lymphocytes, CD5 lymphocytes, and sIgM B lymphocytes.
- There was considerable variation in the recovery of BALF cells, which led to a wide range in the absolute numbers of each lymphocyte subset.
- Despite this variability, the relative proportions of these cells in the BALF were less variable, providing a basis for comparison with peripheral blood lymphocyte populations.
T Lymphocytes vs B Lymphocytes
- The total lymphocyte population was comprised mostly of T cells in both the BALF and peripheral blood, with the portion being significantly larger in the BALF.
- Contrastingly, the proportion of B lymphocytes was significantly lower in the BALF compared to peripheral blood.
Different Types of T Lymphocytes
- The increase in T lymphocytes in the BALF was due to a significantly higher percentage of CD8 T lymphocytes in BALF compared to peripheral blood.
- There was no significant difference between the two compartments in terms of the proportion of CD4 T lymphocytes and CD5 lymphocytes – the latter group is predominantly T lymphocytes but includes a small subset of sIgM B lymphocytes.
Implications of Findings
- The results form baseline values for the lymphocyte populations in the BALF and peripheral blood of normal, adult Arabian mares.
- This allows for the detection of changes associated with disease, as well as determining the effects of factors such as age and breed on lymphocyte subpopulations in the BALF and peripheral blood.
Cite This Article
APA
Hines MT, Palmer GH, Byrne KM, Brassfield AL, McGuire TC.
(1996).
Quantitative characterization of lymphocyte populations in bronchoalveolar lavage fluid and peripheral blood of normal adult Arabian horses.
Vet Immunol Immunopathol, 51(1-2), 29-37.
https://doi.org/10.1016/0165-2427(95)05510-x Publication
Researcher Affiliations
- Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USA.
MeSH Terms
- Animals
- Bronchoalveolar Lavage Fluid / cytology
- Female
- Horses / blood
- Lymphocyte Count / veterinary
- Lymphocyte Subsets
Grant Funding
- AI24291 / NIAID NIH HHS
Citations
This article has been cited 3 times.- Mealey RH, Littke MH, Leib SR, Davis WC, McGuire TC. Failure of low-dose recombinant human IL-2 to support the survival of virus-specific CTL clones infused into severe combined immunodeficient foals: lack of correlation between in vitro activity and in vivo efficacy. Vet Immunol Immunopathol 2008 Jan 15;121(1-2):8-22.
- Lopez AM, Hines MT, Palmer GH, Alperin DC, Hines SA. Identification of pulmonary T-lymphocyte and serum antibody isotype responses associated with protection against Rhodococcus equi. Clin Diagn Lab Immunol 2002 Nov;9(6):1270-6.
- Fraser DG, Oaks JL, Brown WC, McGuire TC. Identification of broadly recognized, T helper 1 lymphocyte epitopes in an equine lentivirus. Immunology 2002 Mar;105(3):295-305.
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