Radioimmunoassay technique for detecting urinary excretion products after administration of synthetic anabolic steroids to the horse.
Abstract: 1. Cross-bred and thoroughbred geldings were injected with veterinary doses of various synthetic anabolic steroids. Urines collected sequentially from treated animals were analysed, following solvent extraction, by radioimmunoassay using 19-[3H]nortestosterone and an antibody raised against a 19-nortestosterone immunogen. 2. Urinary excretion of 19-nortestosterone and/or its cross-reacting metabolites was detectable for various times after administration of different nortestosterone esters, as follows: phenylpropionate (400 mg), greater than 14 days; cyclohexylpropionate (100 mg), greather than 10 days; laurate (200 mg) greater than 50 days. After administration of the parent steroid (150 mg) cross-reacting compounds were detectable in urine for ca. 3 days. 3. Urinary excretion of esters of other anabolic steroids cross-reacting with the 19-nortestosterone antibody (e.g. 1-dehydrotestosterone and trienbolone) could also be followed by analysing solvent extracts of urines by the radioimmunoassay. Cross-reacting compounds in urine after administration of 1-dehydrotestosterone undecylenate (250 mg) and trienbolone acetate (75 mg) could be detected for greater than 35 days and greather than 5 days, respectively.
Publication Date: 1978-04-01 PubMed ID: 645104DOI: 10.3109/00498257809056141Google Scholar: Lookup
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- Journal Article
Summary
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The research article deals with a method called radioimmunoassay to detect the presence of synthetic anabolic steroids in horse urine. The findings showed that different types of steroids and their doses resulted in variable detection periods in the urine.
Research Methodology
- The study involved cross-bred and thoroughbred geldings, who were injected with varying doses of different synthetic anabolic steroids.
- The urine of the treated animals was collected over a period of time and analysed post solvent extraction using a radiation technique called radioimmunoassay. The radioimmunoassay used was a 19-[3H]nortestosterone and an antibody raised against a 19-nortestosterone immunogen.
Findings
- The research found varying detectable periods of excretion for different steroids: 19-nortestosterone and its cross-reacting metabolites were detectable for more than 14 days, more than 10 days, and more than 50 days when phenylpropionate (400 mg), cyclohexylpropionate (100 mg), and laurate (200 mg) were administered respectively. With the injection of the parent steroid (150 mg), cross-reacting compounds were detectable for around 3 days.
- The presence of other anabolic steroid esters that cross-react with the 19-nortestosterone antibody could also be traced by using the radioimmunoassay. In this case, after the administration of 1-dehydrotestosterone undecylenate (250 mg) and trienbolone acetate (75 mg), cross-reacting compounds were detectable for more than 35 days and more than 5 days, respectively.
Implications
- The results of this research demonstrate that the use of synthetic anabolic steroids can be detected in horse urine using the radioimmunoassay method, aiding in monitoring illegal substance use in horse racing.
- The variable detection periods provide valuable insights for the formulation of effective monitoring protocols, considering the type of steroid used and its effective dosage.
- The research also highlights the utility of radioimmunoassay as a sensitive and effective method for tracing even low concentrations of steroids and their metabolites.
- The study involved cross-bred and thoroughbred geldings, who were injected with varying doses of different synthetic anabolic steroids.
- The urine of the treated animals was collected over a period of time and analysed post solvent extraction using a radiation technique called radioimmunoassay. The radioimmunoassay used was a 19-[3H]nortestosterone and an antibody raised against a 19-nortestosterone immunogen.
Findings
- The research found varying detectable periods of excretion for different steroids: 19-nortestosterone and its cross-reacting metabolites were detectable for more than 14 days, more than 10 days, and more than 50 days when phenylpropionate (400 mg), cyclohexylpropionate (100 mg), and laurate (200 mg) were administered respectively. With the injection of the parent steroid (150 mg), cross-reacting compounds were detectable for around 3 days.
- The presence of other anabolic steroid esters that cross-react with the 19-nortestosterone antibody could also be traced by using the radioimmunoassay. In this case, after the administration of 1-dehydrotestosterone undecylenate (250 mg) and trienbolone acetate (75 mg), cross-reacting compounds were detectable for more than 35 days and more than 5 days, respectively.
Implications
- The results of this research demonstrate that the use of synthetic anabolic steroids can be detected in horse urine using the radioimmunoassay method, aiding in monitoring illegal substance use in horse racing.
- The variable detection periods provide valuable insights for the formulation of effective monitoring protocols, considering the type of steroid used and its effective dosage.
- The research also highlights the utility of radioimmunoassay as a sensitive and effective method for tracing even low concentrations of steroids and their metabolites.
- The research found varying detectable periods of excretion for different steroids: 19-nortestosterone and its cross-reacting metabolites were detectable for more than 14 days, more than 10 days, and more than 50 days when phenylpropionate (400 mg), cyclohexylpropionate (100 mg), and laurate (200 mg) were administered respectively. With the injection of the parent steroid (150 mg), cross-reacting compounds were detectable for around 3 days.
- The presence of other anabolic steroid esters that cross-react with the 19-nortestosterone antibody could also be traced by using the radioimmunoassay. In this case, after the administration of 1-dehydrotestosterone undecylenate (250 mg) and trienbolone acetate (75 mg), cross-reacting compounds were detectable for more than 35 days and more than 5 days, respectively.
Implications
- The results of this research demonstrate that the use of synthetic anabolic steroids can be detected in horse urine using the radioimmunoassay method, aiding in monitoring illegal substance use in horse racing.
- The variable detection periods provide valuable insights for the formulation of effective monitoring protocols, considering the type of steroid used and its effective dosage.
- The research also highlights the utility of radioimmunoassay as a sensitive and effective method for tracing even low concentrations of steroids and their metabolites.
- The results of this research demonstrate that the use of synthetic anabolic steroids can be detected in horse urine using the radioimmunoassay method, aiding in monitoring illegal substance use in horse racing.
- The variable detection periods provide valuable insights for the formulation of effective monitoring protocols, considering the type of steroid used and its effective dosage.
- The research also highlights the utility of radioimmunoassay as a sensitive and effective method for tracing even low concentrations of steroids and their metabolites.
Cite This Article
APA
Jondorf WR, Moss MS.
(1978).
Radioimmunoassay technique for detecting urinary excretion products after administration of synthetic anabolic steroids to the horse.
Xenobiotica, 8(4), 197-206.
https://doi.org/10.3109/00498257809056141 Publication
Researcher Affiliations
MeSH Terms
- Anabolic Agents / urine
- Animals
- Cross Reactions
- Estrenes / urine
- Horses / urine
- Male
- Methods
- Nandrolone / analogs & derivatives
- Nandrolone / urine
- Radioimmunoassay
- Testosterone / analogs & derivatives
- Testosterone / immunology
- Testosterone / urine
- Time Factors
Citations
This article has been cited 1 times.- Jondorf WR. On the raising of antibody to the synthetic anabolic steroid trienbolone, its partial characterization and preliminary application for radioimmunoassay.. Experientia 1980 Apr 15;36(4):394-5.
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