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Home / Equine Research Bank / Vet Anim Sci / Rapid detection of alveolar echinococcosis in hepatic nodule...
Veterinary and animal science2023; 20; 100291; doi: 10.1016/j.vas.2023.100291

Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay.

Abstract: Alveolar echinococcosis in slaughtered horses remains a public health issue. This study aimed to develop a Recombinase polymerase amplification (RPA) assay targeting the mitochondrial NADH dehydrogenase subunit 5 () gene of for the rapid detection of equine alveolar echinococcosis. Thirty-six hepatic solid nodules obtained from each horse ( = 36) were evaluated based on histopathological examination and -targeted PCR and then submitted to the RPA assay. The results of the developed RPA assay were 94.4% consistent with those of PCR and Cohen's kappa coefficient value was 0.89 statistically, indicating high agreement. In addition, the RPA assay using the plasmid samples was one hundredfold more sensitive than PCR testing. Consequently, these results suggest that the performance of the RPA assay developed in this study is equal to that of conventional PCR testing.
© 2023 The Author(s).
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Publication Date: 2023-03-02 PubMed ID: 36936549PubMed Central: PMC10015249DOI: 10.1016/j.vas.2023.100291Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article is about developing a rapid detection method for alveolar echinococcosis in horses using a Recombinase Polymerase Amplification (RPA) assay, which showed high accuracy and greater sensitivity than traditional PCR testing.

Objective of the Study

  • The aim of this research is to establish an RPA assay that targets the mitochondrial NADH dehydrogenase subunit 5 gene of Echinococcus multilocularis, the parasite that causes alveolar echinococcosis in horses. The study is rooted in the continuing public health issue of alveolar echinococcosis found in slaughtered horses.

Methodology

  • 36 hepatic nodules from different horses were collected for the study.
  • All the samples underwent histopathological examination and gene-targeted PCR testing.
  • After these preliminary examinations, the samples were then submitted to the newly developed RPA assay.

Results

  • The results found from the RPA assay matched 94.4% of the time with the PCR test results, indicating high concordance between these two methods.
  • The statistical agreement between the two tests was high with a Cohen’s kappa coefficient value of 0.89.
  • The RPA assay also demonstrated a sensitivity that was 100 times greater than the sensitivity of the PCR testing when the plasmid samples were used.

Conclusion

  • The study concludes that this newly developed RPA assay has a performance equivalent to that of the conventional PCR tests. However, its sensitivity appears to be significantly higher.
  • Suggesting that this new RPA-based method could be an effective diagnostic tool for rapidly detecting alveolar echinococcosis in horses.

Cite This Article

APA
Hifumi T, Tanaka T, Sato M, Akioka K, Fujimata C, Miyoshi N. (2023). Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay. Vet Anim Sci, 20, 100291. https://doi.org/10.1016/j.vas.2023.100291

Publication

Veterinary and animal science
ISSN: 2451-943X
NlmUniqueID: 101694897
Country: Netherlands
Language: English
Volume: 20
Pages: 100291
PII: 100291

Researcher Affiliations

Hifumi, Tatsuro
  • Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Tanaka, Tetsuya
  • Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Sato, Miho
  • Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Akioka, Kohei
  • Kumamoto Prefectural Meat Inspection Center, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto 861-1344, Japan.
Fujimata, Chiaki
  • Kumamoto Prefectural Meat Inspection Center, 1314 Sosaki, Shichijo-machi, Kikuchi, Kumamoto 861-1344, Japan.
Miyoshi, Noriaki
  • Laboratory of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Conflict of Interest Statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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