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Home / Equine Research Bank / J Orthop Surg Res / Rationale and pre-clinical evidences for the use of autologo...
Journal of orthopaedic surgery and research2018; 13(1); 279; doi: 10.1186/s13018-018-0983-y

Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair.

Abstract: The management of cartilage lesions is an open issue in clinical practice, and regenerative medicine represents a promising approach, including the use of autologous micrografts whose efficacy was already tested in different clinical settings. The aim of this study was to characterize in vitro the effect of autologous cartilage micrografts on chondrocyte viability and differentiation and perform an evaluation of their application in racehorses affected by joint diseases. Methods: Matched human chondrocytes and micrografts were obtained from articular cartilage using Rigenera® procedure. Chondrocytes were cultured in the presence or absence of micrografts and chondrogenic medium to assess cell viability and cell differentiation. For the pre-clinical evaluation, three racehorses affected by joint diseases were treated with a suspension of autologous micrografts and PRP in arthroscopy interventions. Clinical and radiographic follow-ups were performed up to 4 months after the procedure. Results: Autologous micrografts support the formation of chondrogenic micromasses thanks to their content of matrix and growth factors, such as transforming growth factor β (TGFβ) and insulin-like growth factor 1 (IGF-1). On the other hand, no significant differences were observed on the gene expression of type II collagen, aggrecan, and SOX9. Preliminary data in the treatment of racehorses are suggestive of a potential in vivo use of micrografts to treat cartilage lesions. Conclusions: The results reported in this study showed the role of articular micrografts in the promoting chondrocyte differentiation suggesting their potential use in the clinical practice to treat articular lesions.
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Publication Date: 2018-11-06 PubMed ID: 30400946PubMed Central: PMC6218996DOI: 10.1186/s13018-018-0983-yGoogle Scholar: Lookup
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  • Clinical Trial
  • Veterinary
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the use of autologous cartilage micrografts in cartilage repair and their efficacy in treating joint diseases in racehorses. The study found that these micrografts promote chondrocyte differentiation, presenting a potential solution for the treatment of cartilage lesions.

Methods

  • The scientists obtained matched human chondrocytes and micrografts from articular cartilage using a process called the Rigenera® procedure.
  • The chondrocytes were then cultured in either presence or absence of the micrografts along with a chondrogenic medium to evaluate cell viability and differentiation.
  • For an in vivo examination, racehorses afflicted with joint diseases were treated with an autologous micrografts and PRP suspension in arthroscopy interventions.
  • Researchers conducted clinical and radiographic follow-ups up to four months after the procedure.

Results

  • The micrografts supported the formation of chondrogenic micromasses due to their content of matrix and growth factors such as transforming growth factor β (TGFβ) and insulin-like growth factor 1 (IGF-1). These growth factors play crucial roles in cell differentiation and proliferation.
  • No significant differences, however, were observed in the gene expression of primary cartilage components like type II collagen, aggrecan, and SOX9.
  • The pre-clinical data showed that treating the racehorses with micrografts had positive indications, implying the potential for in vivo use of micrografts in the treatment of cartilage lesions.

Conclusions

  • The results suggested that articular micrografts could play a significant role in promoting chondrocyte differentiation.
  • These findings insinuate the potential application of articular micrografts in the treatment of articular lesions in clinical practice.

Cite This Article

APA
Viganò M, Tessaro I, Trovato L, Colombini A, Scala M, Magi A, Toto A, Peretti G, de Girolamo L. (2018). Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair. J Orthop Surg Res, 13(1), 279. https://doi.org/10.1186/s13018-018-0983-y

Publication

Journal of orthopaedic surgery and research
ISSN: 1749-799X
NlmUniqueID: 101265112
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 279
PII: 279

Researcher Affiliations

Viganò, Marco
  • IRCCS Istituto Ortopedico Galeazzi, via Riccardo Galeazzi 4, 20161, Milan, Italy.
Tessaro, Irene
  • IRCCS Istituto Ortopedico Galeazzi, via Riccardo Galeazzi 4, 20161, Milan, Italy.
Trovato, Letizia
  • Human Brain Wave, corso Galileo Ferraris 63, 10128, Turin, Italy. info@hbwsrl.com.
Colombini, Alessandra
  • IRCCS Istituto Ortopedico Galeazzi, via Riccardo Galeazzi 4, 20161, Milan, Italy.
Scala, Marco
  • Primus Gel srl, Via Casaregis, 30, 16129, Genoa, Italy.
Magi, Alberto
  • Clinica Veterinaria San Rossore, via delle cascine 149, 56100, Pisa, Italy.
Toto, Andrea
  • Clinica Veterinaria San Rossore, via delle cascine 149, 56100, Pisa, Italy.
Peretti, Giuseppe
  • IRCCS Istituto Ortopedico Galeazzi, via Riccardo Galeazzi 4, 20161, Milan, Italy.
  • Department of Biomedical Sciences for Health, Università degli Studi di Milano, via Mangiagalli 31, 20133, Milan, Italy.
de Girolamo, Laura
  • IRCCS Istituto Ortopedico Galeazzi, via Riccardo Galeazzi 4, 20161, Milan, Italy.

MeSH Terms

  • Animals
  • Arthroscopy
  • Autografts
  • Cartilage / transplantation
  • Cell Differentiation
  • Cell Survival
  • Chondrocytes / physiology
  • Female
  • Horse Diseases / surgery
  • Horses
  • Humans
  • Joint Diseases / surgery
  • Joint Diseases / veterinary
  • Male
  • Platelet-Rich Plasma
  • Primary Cell Culture

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Experimental research on horses comply with institutional, national, or international guidelines. For primary chondrocytes isolation, all individuals provided informed consent as per the Institutional Review Board approved procedure (M-SPER-014.ver7). CONSENT FOR PUBLICATION: Not applicable COMPETING INTERESTS: The author Letizia Trovato is a consultant of the Scientific Division of Human Brain Wave, the company owner of Rigenera™ technology. The other authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 32 references
  1. Ambra LF, de Girolamo L, Mosier B, Gomoll AH. Review: Interventions for Cartilage Disease: Current State-of-the-Art and Emerging Technologies.. Arthritis Rheumatol 2017 Jul;69(7):1363-1373.
    doi: 10.1002/art.40094pubmed: 28294573google scholar: lookup
  2. Badri A, Burkhardt J. Arthroscopic debridement of unicompartmental arthritis: fact or fiction?. Clin Sports Med 2014 Jan;33(1):23-41.
    doi: 10.1016/j.csm.2013.08.008pubmed: 24274843google scholar: lookup
  3. Steinwachs MR, Guggi T, Kreuz PC. Marrow stimulation techniques.. Injury 2008 Apr;39 Suppl 1:S26-31.
    doi: 10.1016/j.injury.2008.01.042pubmed: 18313469google scholar: lookup
  4. Brittberg M. Cell carriers as the next generation of cell therapy for cartilage repair: a review of the matrix-induced autologous chondrocyte implantation procedure.. Am J Sports Med 2010 Jun;38(6):1259-71.
    doi: 10.1177/0363546509346395pubmed: 19966108google scholar: lookup
  5. Devitt BM, Bell SW, Webster KE, Feller JA, Whitehead TS. Surgical treatments of cartilage defects of the knee: Systematic review of randomised controlled trials.. Knee 2017 Jun;24(3):508-517.
    doi: 10.1016/j.knee.2016.12.002pubmed: 28189406google scholar: lookup
  6. Correa D, Lietman SA. Articular cartilage repair: Current needs, methods and research directions.. Semin Cell Dev Biol 2017 Feb;62:67-77.
    doi: 10.1016/j.semcdb.2016.07.013pubmed: 27422331google scholar: lookup
  7. Trovato L, Monti M, Del Fante C, Cervio M, Lampinen M, Ambrosio L, Redi CA, Perotti C, Kankuri E, Ambrosio G, Rodriguez Y Baena R, Pirozzi G, Graziano A. A New Medical Device Rigeneracons Allows to Obtain Viable Micro-Grafts From Mechanical Disaggregation of Human Tissues.. J Cell Physiol 2015 Oct;230(10):2299-303.
    doi: 10.1002/jcp.24973pubmed: 25728337google scholar: lookup
  8. Monti M, Graziano A, Rizzo S, Perotti C, Del Fante C, d'Aquino R, Redi CA, Rodriguez Y Baena R. In Vitro and In Vivo Differentiation of Progenitor Stem Cells Obtained After Mechanical Digestion of Human Dental Pulp.. J Cell Physiol 2017 Mar;232(3):548-555.
    doi: 10.1002/jcp.25452pubmed: 27277190google scholar: lookup
  9. Brunelli G, Motroni A, Graziano A, D'Aquino R, Zollino I, Carinci F. Sinus lift tissue engineering using autologous pulp micro-grafts: A case report of bone density evaluation.. J Indian Soc Periodontol 2013 Sep;17(5):644-7.
    doi: 10.4103/0972-124X.119284pmc: PMC3808021pubmed: 24174760google scholar: lookup
  10. D’Aquino R, Trovato L, Graziano A, Ceccarelli G, Cusella de Angelis G, Marangini A, Nisio A, Galli M, Pasi M, Finotti M, Lupi SM, Rizzo S, Rodriguez Y, Baena R. Periosteum-derived micro-grafts for tissue regeneration of human maxillary bone.. J Transl Sci 2016;2(2):125–129.
    doi: 10.15761/JTS.1000128google scholar: lookup
  11. Baglioni E, Trovato L, Marcarelli M, Frenello A, Bocchiotti MA. Treatment of Oncological Post-surgical Wound Dehiscence with Autologous Skin Micrografts.. Anticancer Res 2016 Mar;36(3):975-9.
    pubmed: 26976986
  12. Marcarelli M, Trovato L, Novarese E, Riccio M, Graziano A. Rigenera protocol in the treatment of surgical wound dehiscence.. Int Wound J 2017 Feb;14(1):277-281.
    doi: 10.1111/iwj.12601pubmed: 27126653google scholar: lookup
  13. Trovato L, Failla G, Serantoni S, Palumbo FP. Regenerative surgery in the management of the leg ulcers.. J Cell Sci Ther 2016;7:238.
    doi: 10.4172/2157-7013.1000238google scholar: lookup
  14. De Francesco F, Graziano A, Trovato L, Ceccarelli G, Romano M, Marcarelli M, Cusella De Angelis GM, Cillo U, Riccio M, Ferraro GA. Erratum to: A Regenerative Approach with Dermal Micrografts in the Treatment of Chronic Ulcers.. Stem Cell Rev Rep 2017 Feb;13(1):149.
    doi: 10.1007/s12015-016-9698-9pubmed: 27826795google scholar: lookup
  15. Svolacchia F, De Francesco F, Trovato L, Graziano A, Ferraro GA. An innovative regenerative treatment of scars with dermal micrografts.. J Cosmet Dermatol 2016 Sep;15(3):245-53.
    doi: 10.1111/jocd.12212pubmed: 26825041google scholar: lookup
  16. Gentile P, Scioli MG, Bielli A, Orlandi A, Cervelli V. Reconstruction of Alar Nasal Cartilage Defects Using a Tissue Engineering Technique Based on a Combined Use of Autologous Chondrocyte Micrografts and Platelet-rich Plasma: Preliminary Clinical and Instrumental Evaluation.. Plast Reconstr Surg Glob Open 2016 Oct;4(10):e1027.
    doi: 10.1097/GOX.0000000000001027pmc: PMC5096517pubmed: 27826462google scholar: lookup
  17. Lampinen M, Nummi A, Nieminen T, Harjula A, Kankuri E. Intraoperative processing and epicardial transplantation of autologous atrial tissue for cardiac repair.. J Heart Lung Transplant 2017 Sep;36(9):1020-1022.
    doi: 10.1016/j.healun.2017.06.002pubmed: 28668192google scholar: lookup
  18. Branly T, Bertoni L, Contentin R, Rakic R, Gomez-Leduc T, Desancé M, Hervieu M, Legendre F, Jacquet S, Audigié F, Denoix JM, Demoor M, Galéra P. Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1.. Stem Cell Rev Rep 2017 Oct;13(5):611-630.
    doi: 10.1007/s12015-017-9748-ypubmed: 28597211google scholar: lookup
  19. Cokelaere S, Malda J, van Weeren R. Cartilage defect repair in horses: Current strategies and recent developments in regenerative medicine of the equine joint with emphasis on the surgical approach.. Vet J 2016 Aug;214:61-71.
    doi: 10.1016/j.tvjl.2016.02.005pubmed: 27387728google scholar: lookup
  20. Barrett JG. A Set of Grand Challenges for Veterinary Regenerative Medicine.. Front Vet Sci 2016;3:20.
    doi: 10.3389/fvets.2016.00020pmc: PMC4777877pubmed: 26973846google scholar: lookup
  21. Lopa S, Ceriani C, Cecchinato R, Zagra L, Moretti M, Colombini A. Stability of housekeeping genes in human intervertebral disc, endplate and articular cartilage cells in multiple conditions for reliable transcriptional analysis.. Eur Cell Mater 2016 May 27;31:395-406.
    doi: 10.22203/eCM.v031a25pubmed: 27232666google scholar: lookup
  22. Scala M, Lenarduzzi S, Spagnolo F, Trapasso M, Ottonello C, Muraglia A, Barla A, Squillario M, Strada P. Regenerative medicine for the treatment of Teno-desmic injuries of the equine. A series of 150 horses treated with platelet-derived growth factors.. In Vivo 2014 Nov-Dec;28(6):1119-23.
    pubmed: 25398809
  23. Freyria AM, Mallein-Gerin F. Chondrocytes or adult stem cells for cartilage repair: the indisputable role of growth factors.. Injury 2012 Mar;43(3):259-65.
    doi: 10.1016/j.injury.2011.05.035pubmed: 21696723google scholar: lookup
  24. Bernhard JC, Vunjak-Novakovic G. Should we use cells, biomaterials, or tissue engineering for cartilage regeneration?. Stem Cell Res Ther 2016 Apr 18;7(1):56.
    doi: 10.1186/s13287-016-0314-3.pmc: PMC4836146pubmed: 27089917google scholar: lookup
  25. Ceccarelli G, Gentile P, Marcarelli M, Balli M, Ronzoni FL, Benedetti L, Cusella De Angelis MG. In Vitro and In Vivo Studies of Alar-Nasal Cartilage Using Autologous Micro-Grafts: The Use of the Rigenera(®) Protocol in the Treatment of an Osteochondral Lesion of the Nose.. Pharmaceuticals (Basel) 2017 Jun 13;10(2).
    doi: 10.3390/ph10020053pmc: PMC5490410pubmed: 28608799google scholar: lookup
  26. Rodriguez Y Baena R, D'Aquino R, Graziano A, Trovato L, Aloise AC, Ceccarelli G, Cusella G, Pelegrine AA, Lupi SM. Autologous Periosteum-Derived Micrografts and PLGA/HA Enhance the Bone Formation in Sinus Lift Augmentation.. Front Cell Dev Biol 2017;5:87.
    doi: 10.3389/fcell.2017.00087pmc: PMC5623661pubmed: 29021982google scholar: lookup
  27. Purpura V, Bondioli E, Graziano A, Trovato L, Melandri D, Ghetti M, Marchesini A, Cusella De Angelis MG, Benedetti L, Ceccarelli G, Riccio M. Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation.. J Vis Exp 2016 Mar 4;(109):e53579.
    pmc: PMC4828224pubmed: 26967938doi: 10.3791/53579google scholar: lookup
  28. Goldberg A, Mitchell K, Soans J, Kim L, Zaidi R. The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review.. J Orthop Surg Res 2017 Mar 9;12(1):39.
    doi: 10.1186/s13018-017-0534-ypmc: PMC5345159pubmed: 28279182google scholar: lookup
  29. Sermer C, Devitt B, Chahal J, Kandel R, Theodoropoulos J. The Addition of Platelet-Rich Plasma to Scaffolds Used for Cartilage Repair: A Review of Human and Animal Studies.. Arthroscopy 2015 Aug;31(8):1607-25.
    doi: 10.1016/j.arthro.2015.01.027pubmed: 25823672google scholar: lookup
  30. Serra CI, Soler C, Carrillo JM, Sopena JJ, Redondo JI, Cugat R. Effect of autologous platelet-rich plasma on the repair of full-thickness articular defects in rabbits.. Knee Surg Sports Traumatol Arthrosc 2013 Aug;21(8):1730-6.
    doi: 10.1007/s00167-012-2141-0pubmed: 22918398google scholar: lookup
  31. Filardo G, Kon E, Buda R, Timoncini A, Di Martino A, Cenacchi A, Fornasari PM, Giannini S, Marcacci M. Platelet-rich plasma intra-articular knee injections for the treatment of degenerative cartilage lesions and osteoarthritis.. Knee Surg Sports Traumatol Arthrosc 2011 Apr;19(4):528-35.
    doi: 10.1007/s00167-010-1238-6pubmed: 20740273google scholar: lookup
  32. Pallante AL, Chen AC, Ball ST, Amiel D, Masuda K, Sah RL, Bugbee WD. The in vivo performance of osteochondral allografts in the goat is diminished with extended storage and decreased cartilage cellularity.. Am J Sports Med 2012 Aug;40(8):1814-23.
    doi: 10.1177/0363546512449321pmc: PMC4041579pubmed: 22707746google scholar: lookup

Citations

This article has been cited 4 times.
  1. Desando G, Grigolo B, Deangelles Pereira Florentino Á, Teixeira MW, Barbagallo F, Naro F, da Silva-Júnior VA, Soares AF. Preclinical Evidence of Intra-Articular Autologous Cartilage Micrograft for Osteochondral Repair: Evaluation in a Rat Model.. Cartilage 2021 Dec;13(2_suppl):1770S-1779S.
    doi: 10.1177/19476035211042408pubmed: 34474579google scholar: lookup
  2. Marcarelli M, Zappia M, Rissolio L, Baroni C, Astarita C, Trovato L, Graziano A. Cartilage Micrografts as a Novel Non-Invasive and Non-Arthroscopic Autograft Procedure for Knee Chondropathy: Three-Year Follow-Up Study.. J Clin Med 2021 Jan 17;10(2).
    doi: 10.3390/jcm10020322pubmed: 33477260google scholar: lookup
  3. Araújo CRG, Astarita C, D'Aquino R, Pelegrine AA. Evaluation of Bone Regeneration in Rat Calvaria Using Bone Autologous Micrografts and Xenografts: Histological and Histomorphometric Analysis.. Materials (Basel) 2020 Sep 25;13(19).
    doi: 10.3390/ma13194284pubmed: 32992850google scholar: lookup
  4. Astarita C, Arora CL, Trovato L. Tissue regeneration: an overview from stem cells to micrografts.. J Int Med Res 2020 Jun;48(6):300060520914794.
    doi: 10.1177/0300060520914794pubmed: 32536230google scholar: lookup
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