Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus.
Abstract: Equine influenza is a cause of epizootic respiratory disease of the equine. The detection of equine influenza virus using real-time Light Cycler reverse transcription (RT)-PCR technology was evaluated over two influenza seasons with the analysis of 171 samples submitted for viral respiratory disease. Increased sensitivity was found in overall viral detection with this system compared to Directigen Flu A and virus isolation, which were 40% and 23%, respectively, that of the RT-PCR. The assay was also evaluated as a viable replacement for the more traditional methods of quantifying equine influenza virus, 50% egg infectious dose and 50% tissue culture infectious dose. There was a significant positive correlation (P<0.05) between the quantitative RT-PCR and both of these assays.
Publication Date: 2005-10-07 PubMed ID: 16207961PubMed Central: PMC1248463DOI: 10.1128/JCM.43.10.5055-5057.2005Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Management
- Disease Outbreaks
- Disease Surveillance
- Epidemiology
- Equine Diseases
- Equine Health
- Infectious Disease
- Influenza
- Laboratory Methods
- Molecular biology
- Polymerase Chain Reaction
- Real-Time PCR
- Respiratory Disease
- Veterinary Medicine
- Veterinary Research
- Virology
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research focuses on evaluating the utilization of real-time Light Cycler reverse transcription-PCR for the detection and quantitative analysis of the equine influenza virus. The study found that this method results in higher sensitivity in viral detection compared to Directigen Flu A and virus isolation methods.
Research Methodology
- The study was conducted over two influenza seasons.
- A total of 171 samples that were submitted for respiratory viral disease were analyzed.
- Real-time Light Cycler reverse transcription (RT)-PCR technology was used for equine influenza virus detection.
- The sensitivity of this technology was compared with those of Directigen Flu A and virus isolation, which were both traditional virus detection methods.
Study Findings
- Results showed that the use of real-time RT-PCR provided increased sensitivity in overall viral detection.
- In terms of sensitivity, the RT-PCR detection rate was more than double that of Directigen Flu A and nearly five times that of virus isolation method.
- This implies that the RT-PCR method is able to detect the presence of the virus more accurately and efficiently than the other two methods.
Quantitative Analysis
- The study also evaluated the RT-PCR assay as a potential alternative to the traditional methods of quantifying equine influenza virus: the 50% egg infectious dose and the 50% tissue culture infectious dose.
- A significant positive correlation was found between the quantitative RT-PCR and both of these traditional assays.
- This indicates that RT-PCR could also serve as a viable method for quantifying the amount of equine influenza virus present in a sample.
Study Implications
- According to the study, real-time RT-PCR technology implies a promising possibility of improving the detection and quantification of equine influenza virus.
- This method can potentially improve diagnostic accuracy and provide a more effective approach to manage and control the disease in equine populations.
- However, the study suggests further research to confirm these preliminary findings and to explore other potential benefits of this technology in other aspects of equine influenza virus diagnosis and control.
Cite This Article
APA
Quinlivan M, Dempsey E, Ryan F, Arkins S, Cullinane A.
(2005).
Real-time reverse transcription PCR for detection and quantitative analysis of equine influenza virus.
J Clin Microbiol, 43(10), 5055-5057.
https://doi.org/10.1128/JCM.43.10.5055-5057.2005 Publication
Researcher Affiliations
- Virology Unit, The Irish Equine Centre, Johnstown, Naas, Co. Kildare, Ireland.
MeSH Terms
- Animals
- Horse Diseases / diagnosis
- Horse Diseases / virology
- Horses / virology
- Influenza A Virus, H3N8 Subtype / classification
- Influenza A Virus, H3N8 Subtype / genetics
- Influenza A Virus, H3N8 Subtype / isolation & purification
- Nasopharynx / virology
- Orthomyxoviridae Infections / diagnosis
- Orthomyxoviridae Infections / veterinary
- Orthomyxoviridae Infections / virology
- Reverse Transcriptase Polymerase Chain Reaction / methods
- Sensitivity and Specificity
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Citations
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