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Equine veterinary journal2021; 54(1); 11-23; doi: 10.1111/evj.13512

Recent advancements in our understanding of equid gammaherpesvirus infections.

Abstract: Equid gammaherpesviruses are ubiquitous and widespread in the equine population. Despite their frequent detection, their contribution to immune system modulation and the pathogenesis of several diseases remains unclear. Genetic variability and the combination of equid gammaherpesvirus strains a horse is infected with might be clinically significant. Initial gammaherpesvirus infection occurs in foals peripartum with latency then established in peripheral blood mononuclear cells. A novel EHV-5 study suggests that following inhalation equid gammaherpesviruses might obtain direct access to T and B lymphocytes via the tonsillar crypts to establish latency. EHV-5 is associated with equine multinodular pulmonary fibrosis, however, unlike with EHV-2 there is currently minimal evidence for its role in milder cases of respiratory disease and poor performance. Transmission is presumed to be via the upper respiratory tract with periodic reactivation of the latent virus in adult horses. Stress of transport has been identified as a risk factor for reactivation and shedding of equine gammaherpesviruses. There is currently a lack of evidence for the effectiveness of antiviral drugs in the treatment of equine gammaherpesvirus infections.
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Publication Date: 2021-10-01 PubMed ID: 34519074DOI: 10.1111/evj.13512Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Equid gammaherpesviruses prevalent in horses across the world are now understood to play a role in modulating their immune system and contributing to diseases. Yet, more research is needed on the impact of genetic variability and combination of virus strains infecting a horse. A study suggests the transmission route could be through the respiratory tract, directly to T and B lymphocytes via tonsillar crypts. This research opens discussion on potential antiviral treatments and the influence of stress on virus reactivation and shedding.

Gammaherpesvirus Infections in Horses

  • Equid gammaherpesviruses are pervasive in the worldwide horse population.
  • While these viruses are frequently detected, their specific roles in modulating the immune system and the development of various equine diseases are not yet fully understood.

Genetic Variability and Strain Combination

  • The study highlights that the genetic variability of gammaherpesviruses, and the combination of different strains a horse is infected with, can have potentially significant clinical impacts.

Transmission and Latency

  • The initial infection of gammaherpesviruses happens peripartum in foals, after which latency is established in their peripheral blood mononuclear cells.
  • A pioneering study on EHV-5 indicates that, upon inhalation, equid gammaherpesviruses might directly access T and B lymphocyte cells through tonsillar crypts to establish latency.
  • EHV-5 is linked with equine multinodular pulmonary fibrosis (EMPF), but as it stands, there’s limited evidence suggesting its role in less severe respiratory diseases or poor performance.
  • The assumed mode of virus transmission is through the upper respiratory tract, with occasional reactivation of the latent virus in adult horses.

Stress-induced Reactivation and Virus Shedding

  • The research identified the stress of transportation as a risk factor for the reactivation and shedding of equine gammaherpesviruses.

Therapeutic Possibilities and Limitations

  • One significant finding includes the lack of evidence supporting the efficacy of antiviral drugs in the treatment of equine gammaherpesvirus infections.

Cite This Article

APA
Easton-Jones C. (2021). Recent advancements in our understanding of equid gammaherpesvirus infections. Equine Vet J, 54(1), 11-23. https://doi.org/10.1111/evj.13512

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 1
Pages: 11-23

Researcher Affiliations

Easton-Jones, Charlotte
  • Rossdales LLP, Newmarket, UK.

MeSH Terms

  • Animals
  • Gammaherpesvirinae / genetics
  • Herpesviridae Infections / veterinary
  • Herpesvirus 1, Equid
  • Horse Diseases
  • Horses
  • Leukocytes, Mononuclear
  • Nose

References

This article includes 178 references
  1. Bell SA, Balasuriya UBR, Gardner IA, Barry PA, Wilson WD, Ferraro GL. Temporal detection of equine herpesvirus infections of a cohort of mares and their foals.. Vet Microbiol 2006;116:249-57.
  2. Torfason EG, Thorsteinsdottir L, Torsteinsdottir S, Svansson V. Study of equid herpesviruses 2 and 5 in Iceland with a type-specific polymerase chain reaction.. Res Vet Sci 2008;85:605-11.
  3. Borchers K, Wolfinger U, Goltz M, Broll H, Ludwig H. Distribution and relevance of equine herpesvirus type 2 (EHV-2) infections.. Arch Virol 1997;142:917-28.
  4. Ruszczyk A, Cywinska A, Banbura MW. Equine herpes virus 2 infection in horse populations in Poland.. Acta Virol 2004;48:189-92.
  5. Franchini M, Akens N, Bracher V, von Fellenberg R. Characterisation of gamma herpesviruses in the horse by PCR.. Virol 1997;238:8-13.
  6. McGuire TC, Crawford TB, Henson JB. Prevalence of antibodies to herpesvirus types 1 and 2, arteritis and infectious anemia viral antigens in equine serum.. Am J Vet Res 1974;35:181-5.
  7. Nordengrahn A, Merza M, Ros C, Lindholmc A, Palfl V, Hannant D. Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays.. Vet Res 2002;33:251-9.
  8. Hartley CA, Dynon KJ, Mekuria ZH, El-Hage CM, Holloway SA, Gilkerson JR. Equine gammaherpesviruses: perfect parasites?. Vet Microbiol 2013;113:2111-222.
  9. Wang L, Raidal SL, Pizzirani A, Wilcox GE. Detection of respiratory herpesviruses in foals and adult horses determined by nested multiplex PCR.. Vet Microbiol 2007;121:18-28.
  10. Borchers K, Frolich K, Ludwig H. Detection of equine herpesvirus types 2 and 5 (EHV-2 and EHV-5) in Przewalski's wild horses.. Arch Virol 1999;144:771-80.
  11. Borchers K, Ebert M, Fetsch A, Hammond T, Sterner-Kock A. Prevalence of equine herpesvirus type 2 (EHV-2) DNA in ocular swabs and its cells tropism in equine conjunctiva.. Vet Microbiol 2006;118:260-6.
  12. Dunowska M, Wilks CR, Syuddert MJ, Meers J. Equine respiratory viruses in foals in New Zealand.. NZ Vet J 2002;50:140-7.
  13. Davison AJ, Eberle R, Ehlers B, Hayward GS, McGeoch DJ, Minson AC. The order herpesvirales.. Adv Virol 2009;154:171-7.
  14. Kleiboeker SB, Schommer SK, Johnson PJ, Ehlers B, Turnquist SE, Boucher M. Association of two newly recognized herpesviruses with interstitial pneumonia in donkeys (Equus asinus).. J Vet Diagn Invest 2002;14(4):273-80.
  15. Schrenzel MD, Tucker TA, Donovan TA, Busch MDM, Wise AG, Maes RK. New hosts for equine herpesvirus 9.. Emerg Infect Dis 2008;14(10):1616-9.
  16. Plummer G, Waterson AP. Equine herpes viruses.. Virol 1963;19:412-6.
  17. Roizman B, Carmichael LE, Deinhardt F, DeThe G, Nahmias AJ, Plowright W. Definition, provisional nomenclature, and taxonomy.. Intervirol 1981;16:201-17.
  18. Browning GF, Studdert MJ. Genomic heterogeneity of equine betaherpesviruses.. J Gen Virol 1987;68:1441-7.
  19. Roizman B, Baines J. The diversity and unity of Herpesviridae.. Comp Immunol Microbiol Infect Dis 1991;14:63-79.
  20. Telford EAR, Studdert MJ, Agius CT. Equine herpesviruses 2 and 5 are gamma-herpesviruses.. Virol 1993;195:492-9.
  21. Telford EA, Watson MS, Aird HC, Perry J, Davison AJ. The DNA sequence of equine herpesvirus 2.. J Mol Biol 1995;249:520-8.
  22. Allen GP, Murray MJ. Equid herpesvirus 2 and equid herpesvirus 5 infections.. 2004. In: Coetzer JAW, Tustin RC, editors. Infectious diseases of livestock. Cape Town: Oxford Press; p. 860-7.
  23. Gulati BR, Sharma H, Riyesh T, Khurana SK, Kapoor S. Viral and host strategies for regulation of latency and reactivation in equid herpesviruses.. Asian J Anim Vet Adv 2015;10:669-89.
  24. Brault SA, Bird BH, Balasuriya UB, MacLachlan NJ. Genetic heterogeneity and variation in viral load during equid herpesvirus-2 infection of foals.. Vet Microbiol 2011;147:253-61.
  25. Thorsteinsdóttir L, Torfason EG, Torsteinsdottir S, Svansson V. Genetic diversity of equine gammaherpesviruses (gamma-EHV) and isolation of a syncytium forming EHV-2 strain from a horse in Iceland.. Res Vet Sci 2013;94:170-7.
  26. Holloway SA, Lindquester GJ, Studdert MJ, Drummer HE. Analysis of equine herpesvirus 2 strain variation using monoclonal antibodies to glycoprotein B.. Arch Virol 2000;145:1699-713.
  27. Back H, Ullman K, Mikael L, Söderlund R, Penell J, Ståhl K. Genetic variation and dynamics of infections of equid herpesvirus 5 individual horses.. J Gen Virol 2016;97:169-78.
  28. Stasiak K, Dunowska M, Trewick S, Rola J. Genetic variation in the glycoprotein B sequence of equid herpesvirus 5 among horses of various breeds at Polish national studs.. Pathogens 2021;10:322.
  29. Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
  30. Fortier G, van Erck E, Pronost S, Lekeux P, Thiry E. Equine gammaherpesviruses: pathogenesis, epidemiology and diagnosis.. Vet J 2010;186:148-56.
  31. Kwok H, Wu CW, Palser AL, Kellam P, Sham PC, Kwong DLW. Genomic diversity of Epstein-Barr virus genomes isolated from primary nasopharyngeal carcinoma biopsy samples.. J Virol 2014;88:10662-72.
  32. Williams KJ. Gammaherpesviruses and pulmonary fibrosis: Evidence from humans, horses and rodents.. Vet Pathol 2014;51(2):372-84.
  33. Grinde B. Herpesviruses: latency and reactivation-viral strategies and host response.. J Oral Microbiol 2013;5.
    doi: 10.3402/jom.v5i0.22766google scholar: lookup
  34. Banks TA, Rouse BT. Herpesviruses-immune escape artists?. Clin Infect Dis 1992;14:933-41.
  35. Ma G, Azab W, Osterrieder N. Equine Herpesviruses type 1 (EHV-1) and 4 (EHV-4) masters of co-evolution and a constant threat to equids and beyond.. Vet Microbiol 2013;167:123-34.
  36. Pusterla N, Mapes S, Wilson WD. Prevalence of latent alpha-herpesviruses in thoroughbred racing horses.. Vet J 2012;193:579-82.
  37. Rushton JO, Kolodziejek J, Tichy A, Nell B, Nowotny N. Detection of equid herpesviruses 2 and 5 in a herd of 266 Lipizzaners in association with ocular findings.. Vet Microbiol 2013;164:139-44.
  38. Drummer HE, Reubel GH, Studdert MJ. Equine gammaherpesvirus 2 (EHV2) is latent in B lymphocytes.. Arch Virol 1996;141:495-504.
  39. Dunowska M, Meers J, Johnson RD, Wilks CR. Influence of equine herpesvirus type 2 infection on monocyte chemoattractant protein 1 gene transcription in equine blood mononuclear cells.. Res Vet Sci 2001;71:111-3.
  40. Brackmann J. The equine herpesvirus type 2 (EHV-2) interleukin-10 homologue: activity during acute and latent infections with special consideration on gene expression in vivo and in vitro.. Das Interleukin 2005;10.
  41. Camarda G, Spinetti G, Bernardini G, Mair C, Davis-Poynter N, Capogrossi MC. The equine herpesvirus 2 E1 open reading frame encodes a functional chemokine receptor.. J Virol 1999;73:9843-8.
  42. Rosenkilde MM, Kledal TN, Schwartz TW. High constitutive activity of a virus-encoded seven transmembrane receptor in the absence of the conserved DRY motif (Asp-Arg-Tyr) in transmembrane helix 3.. Mol. Pharmacol. 2005;68:11-9.
  43. Sharp EL, Farrell HE, Borchers K, Homes EC, Davis-Poynter NJ. Sequence analysis of the equid herpesvirus 2 chemokine receptor homologues E1, ORF74 and E6 demonstrates high sequence divergence between field isolates.. J Gen Virol 2007;88:2450-62.
  44. Bertin J, Armstrong RC, Ottilie S, Martin DA, Wang Y, Banks S. Death effector domain-containing herpesvirus and poxvirus proteins inhibit both fas- and TNFR1- induced apoptosis.. Proc Natl Acad Sci USA 1997;94:1172-6.
  45. Hardwick JM, Bellow DS. Viral versus cellular BCL-2 proteins.. Cell Death Differ 2003;10(Suppl 1):S68-76.
  46. Nakagiri S, Murakami A, Takada S, Akiyama T, Yonehara S. Viral FLIP enhances Wnt signalling downstream of stabilized beta-catenin, leading to control of cell growth.. Mol Cell Biol 2005;25:9249-58.
  47. de Waal Malefyt R, Haanen J, Spits H, Roncarolo MG, te Velde A, Figdor C. Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression.. J Exp Med 1991;174:915-24.
  48. Hughes AL. Origin and evolution of viral interleukin-10 and other DNA virus genes with vertebrate homologues.. J Mol Evol 2002;54:90-101.
  49. Richter N, Ebert M, Borchers K. Prevalence of EHV-2 and EHV-5 DNA in ocular and nasal swabs as well as peripheral blood mononuclear cells.. Pferdeheilkunde 2009;25:38-44.
  50. Mekuria ZH, El-Hage C, Ficorilli NP, Washington EA, Gilkerson JR, Hartley CA. Mapping B lymphocytes as major reservoirs of naturally occurring latent equine herpesvirus 5 infection.. J Gen Virol 2017;98:461-70.
  51. Williams KJ, Maes R, Del Piero F, Lim A, Wise A, Bolin DC. Equine multinodular pulmonary fibrosis: a newly recognized herpesvirus-associated fibrotic lung disease.. Vet Pathol 2007;44:849-62.
  52. Wong DM, Belgrave RL, Williams KJ, Del Piero F, Alcott CJ, Bolin SR. Multinodular pulmonary fibrosis in five horses.. J Am Vet Med Assoc 2008;232:898-905.
  53. Williams KJ, Robinson NE, Lim A, Brandenberger C, Maes R, Behan A. Experimental induction of pulmonary fibrosis in horses with the gammaherpesvirus equine herpesvirus 5.. PLoS One 2013;8(10):e777754.
  54. Poth T, Niedermaier G, Hermanns W. Equine multinodular pulmonary fibrosis in association with an EHV-5 infection in 5 horses.. Wiener Tierärztliche Monatsschrift 2009;96:203-8.
  55. Edington N, Welch HM, Griffiths L. The prevalence of latent Equid herpesviruses in the tissues of 40 abattoir horses.. Equine Vet J 1994;26:140-2.
  56. Rizvi SM, Slater JD, Wolfinger U, Borchers K, Field HJ, Slade AJ. Detection and distribution of equine herpesvirus 2 DNA in the central and peripheral nervous systems of ponies.. J Gen Virol 1997;78:1115-8.
  57. Browning GF, Studdert MJ. Epidemiology of equine herpesvirus 2 (equine cytomegalovirus).. J Clin Microbiol 1987;25:13-6.
  58. Dayaram A, Seeber PA, Greenwood AD. Environmental detection and potential transmission of Equine herpesviruses.. Pathogens 2021;10:423.
  59. Seeber PA, Dayaram A, Sicks F, Osterrieder N, Franz M, Greenwood AD. Noninvasive detection of Equid herpesviruses in fecal samples.. Appl Environ Microbiol 2019;85:e02234-18.
  60. Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response.. PLoS One 2019;14(6):e0218576.
    doi: 10.1371/journal.pone.0218576google scholar: lookup
  61. Back H, Ullman K, Treiberg Berndtsson L, Riihimäki M, Penell J, Ståhl K. Viral load of equine herpesviruses 2 and 5 in nasal swabs of actively racing Standardbred trotters: temporal relationship of shedding to clinical findings and poor performance.. Vet Microbiol 2015;179:142-8.
  62. Marenzoni ML, Coppola G, Maranesi M, Passamonti F, Cappelli K, Capomaccio S. Age-dependent prevalence of equid herpesvirus 5 infection.. Vet Res Commun 2010;34:703-8.
    doi: 10.1007/s11259-010-9443-9google scholar: lookup
  63. Hue ES, Fortier GD, Fortier CI, Leon AM, Richard EA, Legrand LJ. Detection and quantitation of equid gammaherpesviruses (EHV-2 EHV-5) in nasal swabs using an accredited standardised quantitative PCR method.. J Virol Methods 2014;198:18-25.
    doi: 10.1016/j.jviromet.2013.12.008google scholar: lookup
  64. Zarski LM, High EA, Nelli RK, Bolin SR, Williams KJ, Hussey G. Development and application of a quantitative PCR assay to study equine herpesvirus 5 invasion and replication in equine tissues in vitro and in vivo.. J Virol Methods 2017;248:44-53.
  65. Nordengrahn A, Rusvai M, Merza M, Ekstrom J, Morein B, Belak S. Equine herpesvirus type 2 (EHV-2) as a predisposing factor for Rhodococcus equi pneumonia in foals: prevention of the bifactorial disease with EHV-2 immunostimulating complexes.. Vet Microbiol 1996;51:55-68.
  66. Dunowska M, Wilks CR, Studdert MJ, Meers J. Viruses associated with outbreaks of equine respiratory disease in New Zealand.. N Z Vet J 2002;50:132-9.
  67. Blakeslee JR, Olsen RG, McAllister ES, Fassbender J, Dennis R. Evidence of respiratory tract infection induced by equine herpesvirus type 2, in the horse.. Can J Microbiol 1975;21:1940-6.
  68. Thein P, Böhm D. Etiology and clinical aspects of a viral keratoconjunctivitis in foals.. Zentralblatt Veterinärmedizin B 1976;23:507-19.
  69. Collinson PN, O'Rielly JL, Ficorilli N, Studdert MJ. Isolation of equine herpesvirus type 2 (equine gammherpesvirus 2) from foals with keratoconjunctivitis.. J Am Vet Med Assoc 1994;205:329-31.
  70. Kershaw O, von Oppen T, Glitz F, Deegen E, Ludwig H, Borchers K. Detection of equine herpesvirus type 2 (EHV-2) in horses with keratoconjunctivitis.. Virus Res 2001;80:93-9.
  71. Krisova S, Tothova K, Molinkova D, Makra Z, Zisopoulou AM. Prevalence of equine herpesvirus 2 (EHV-2) in equine ocular disease.. Acta Vet Brno 2020;89:115-23.
  72. Studdert MJ. Comparative aspects of equine herpesviruses.. Cornell Vet 1974;64:94-122.
  73. Mumford JA, Rossdale PD. Virus and its relationship to the 'poor performance' syndrome.. Equine Vet J 1980;12:3-9.
  74. Fu ZF, Robinson AJ, Horner GW, Dickinson LG, Grimmett JB, Marshall RB. Respiratory disease in foals and the epizootiology of equine herpesvirus type 2 infection.. N Z Vet J 1986;34:152-5.
  75. Wang L. An investigation of the association between herpes viruses and respiratory disease in racehorses in Western Australia.. Murdoch University PhD Thesis 2003.
  76. Murray MJ, Eichorn ES, Dubovi EJ, Levy WB, Cavey DM. Equine herpesvirus type 2: prevalence and seroepidemiology in foals.. Equine Vet J 1996;28:432-6.
  77. Houtsma A, Bedenice D, Pusterla N, Pugliese B, Mapes S, Hoffman AM. Association between inflammatory airway disease of horses and exposure to respiratory viruses: a case control study.. Multidiscip Respir Med 2015;10:33.
  78. Doubli-Bounoua N, Richard EA, Léon A, Pitel PH, Pronost S, Fortier G. Multiple molecular detection of respiratory viruses and associated signs of airway inflammation in racehorses.. Virol J 2016;13:197.
  79. Fortier G, Richard E, Hue E, Fortier C, Pronost S, Pottier D. Long lasting airway inflammation associated with Equid herpesvirus-2 in experimentally challenged horses.. Vet J 2013;197(2):492-5.
  80. Pálfi V, Belák S, Molnár T. Isolation of equine herpesvirus type 2 from foals, showing respiratory symptoms.. Zentralbl Veterinarmed B 1978;25:165-7.
  81. Dynon K, Black WD, Ficorilli N, Hartley CA, Studdert MJ. Detection of viruses in nasal swab samples from horses with acute, febrile, respiratory disease using virus isolation, polymerase chain reaction and serology.. Aust Vet J 2007;85:46-50.
  82. Sugiura T, Fukuzawa Y, Kamada M, Ando Y, Hirasawa K. Isolation of equine herpesvirus type 2 from foals with pneumonitis.. Bulletin of the Equine Research Institute 1983;20:148-53.
  83. Bilge Dagalp S, Babaoglu AR, Ataseven VS, Karapinar Z, Timurkan MO. Determination of presence of equid alpha and gammaherpesvirus infections in foals with respiratory distress.. Ankara ÜNIV Vet Fak Derg 2018;65:63-8.
  84. Varga J, Fodor L, Rusvai M, Soos I, Makrai L. Prevention of Rhodococcus equi pneumonia of foals using two different inactivated vaccines.. Vet Microbiol 1997;56:205-12.
  85. Pusterla N, Mapes S, Wademan C, White A, Hodzic E. Investigation of the role of lesser characterised respiratory viruses associated with upper respiratory tract infections in horses.. Vet Record 2013;172:315.
  86. Fortier G, van Erck E, Fortier C, Richard E, Pottier D, Pronost S. Herpesviruses in respiratory liquids of horses: putative implication in airway inflammation and association with cytological features.. Vet Microbiol 2009;139:34-41.
  87. Smith FL, Watson JL, Spier SJ, Kilcoyne I, Mapes S, Sonder C. Frequency of shedding of respiratory pathogens in horses recently imported to the United States.. J Vet Intern Med 2018;32(4):1436-41.
  88. Azab W, Bedair S, Abdelgawad A, Eschke K, Farag GK, Abdel-Raheim A. Detection of equid herpesviruses among different Arabian horse populations in Egypt.. Vet Med Sci 2019;5(3):361-71.
  89. McBrearty K, Murray A, Dunowska M. A survey of respiratory viruses in New Zealand horses.. N Z Vet J 2013;2013(61):254-61.
  90. Seo M-G, Ouh I-O, Lee SK, Lee J-S, Kwon O-D, Kwak D. Molecular detection and genetic characteristics of equine herpesvirus in Korea.. Pathogens 2020;9:110.
  91. Bond SL, Workentine M, Hundt J, UCVM Class of 2019, Gilkerson JR, Léguillette R. Effects of nebulized dexamethasone on the respiratory microbiota and mycobiota and relative equine herpesvirus-1, 2, 4, 5 in an equine model of asthma.. J Vet Intern Med 2020;34:307-21.
  92. Stasiak K, Dunowska M, Rola J. Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses.. Virol J 2018;15:106.
  93. Pusterla N, Rice M, Henry T, Barnum S, James K. Investigation of the shedding of selected respiratory pathogens in healthy horses presented for routine dental care.. J Vet Dent 2020;37:88-93.
  94. Agnol AMD, Beuttemmuller EA, Pilz D, Leme RA, Saporiti V, Headley SA. Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil.. Braz J Microbiol 2019;50:875-8.
  95. Couetil L, Ivester K, Barnum S, Pusterla N. Equine respiratory viruses, airway inflammation and performance in thoroughbred racehorses.. Vet Microbiol 2021;257:109070.
  96. Svansson V, Roelse M, Olafsdóttir G, Thorsteinsdóttir L, Torfason EG, Torsteinsdóttir S. Immune response against equine gammaherpesvirus in Icelandic horses.. Vet Microbiol 2009;137:363-8.
  97. Brault SA, Blanchard MT, Gardner IA, Stott JL, Pusterla N, Mapes SM. The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness.. Vet Immunol Immunopathol 2010;137:136-41.
  98. Fu ZF, Denby L, Lien DH, Robinson AJ. An enzyme-linked immunosorbent assay (ELISA) for measurement of antibodies against equine herpesvirus 2 in equine sera.. Acta Virol 1987;31:468-74.
  99. Nordengrahn A, Klingeborn B, Lindholm A, Merza M. The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2).. J Vet Diagn Invest 2001;13:389-93.
  100. Gleeson LJ, Coggins L. Equine herpesvirus type-2: cell-virus relationship during persistent cell-associated viraemia.. Am J Vet Res 1985;46:19-23.
  101. Vander Werf K, Davis E. Disease remission in a horse with EHV-5 associated lymphoma.. J Vet Intern Med 2013;27:387-9.
  102. Vander Werf KA, Davis EG, Janardhan K, Bawa B, Bolin S, Almes K. Identification of equine herpesvirus 5 in horses with lymphoma.. J Equine Vet Sci 2014;34:738-41.
  103. Bawa B, Vander Werf K, Beard L, Davis E, Andrews G, Almes K. Equine multinodular pulmonary fibrosis and lymphoma in a horse associated with equine herpesvirus-5.. J Equine Vet Sci 2014;34:694-700.
  104. Schwarz B, Gruber A, Benetka V, Rutgen B, Schwendenwein I, Leidinger E. Concurrent T cell leukaemia and equine multinodular pulmonary fibrosis in a Hanoverian Warmblood Mare.. Equine Vet Educ 2012;24:187-92.
  105. Herder V, Barsnick R, Walliser U, Teifke J-P, König P, Czerwinski G. Equid herpesvirus 5-associated dermatitis in a horse- Resembling herpes-associated erythema multiforme.. Vet Microbiol 2012;155:420-4.
  106. Kutasi O, Moravszki L, Sardi S, Bohak Z, Biksi I, Basaka F. Systemic granulomatous disease in a Hungarian Warmblood gelding.. J Equine Vet Sci 2014;34:810-5.
  107. Fortier G, Pronost S, Miszczak F, Fortier C, Léon A, Richard E. Identification of equid herpesvirus-5 in respiratory liquids: a retrospective study of 785 samples taken in 2006-2007.. Vet J 2009;182:346-8.
  108. El-Hage CM, Mekuria ZH, Hartley CA, Gilkerson JR. EHV-5 associated with respiratory disease in a survey of Alpha- and Gammaherpesviruses in 409 Australian horses.. Equine Vet J 2014;46(Suppl 47):13.
  109. Pusterla N, Magdesian KG, Mapes SM, Zavodovskaya R, Kass PH. Assessment of quantitative polymerase chain reaction for equine herpesvirus-5 in blood, nasal secretions and bronchoalveolar lavage fluid for the laboratory diagnosis of equine multinodular pulmonary fibrosis.. Equine Vet J 2017;49:34-8.
  110. Akkutay AZ, Osterrieder N, Damiani A, Tischer BK, Borchers K, Alkan F. Prevalence of equine gammaherpesviruses on breeding farms in Turkey and development of a TaqMan MGB real-time PCR to detect equine herpesvirus 5 (EHV-5).. Arch Virol 2014;159:2989-95.
    doi: 10.1007/s00705-014-2165-5google scholar: lookup
  111. Van Cleemput J, Poelaert CK, Laval K, Nauwynck HJ. Unravelling the first key steps in equine herpesvirus type 5 (EHV5) pathogenesis using ex vivo and in vitro equine models.. Vet Res 2019;50:13.
    doi: 10.1186/s13567-019-0630-6google scholar: lookup
  112. Coleman CB, Nealy MS, Tibbetts SA. Immature and transitional B cells are latency reservoirs for gammaherpesvirus.. J Virol 2010;84924:13045-52.
  113. Moser JM, Upton JW, Allen RD, Wilson CB, Speck SH. Role of B-cell proliferation in the establishment of gammaherpesvirus latency.. J Virol 2005;79(15):9480-91.
  114. Flaño E, Husain SM, Sample JT, Woodland DL, Blackman MA. Latent murine gammaherpesvirus infections established in activated B cells, dendritic cells and macrophages.. J Immunol 2000;165(2):1074-81.
  115. Imai S, Nishikawa J, Takada K. Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells.. J Virol 1998;72:4371-8.
  116. Sattentau Q. Avoiding the void: cell-to-cell spread of human viruses.. Nat Rev Microbiol 2008;6:815-26.
  117. Easton-Jones CA, Cissell DD, Mohr FC, Chigerwe M, Pusterla N. Prognostic indicators and long-term survival in 14 horses with equine multinodular pulmonary fibrosis.. Equine Vet Educ 2020;32(S11):41-6.
    doi: 10.1111/eve.13204google scholar: lookup
  118. Spelta CW, Axon JE, Begg A, Diallo ISI, Carrick JB, Russell CM. Equine multinodular pulmonary fibrosis in three horses in Australia.. Aust Vet J 2013;91(7):274-80.
  119. Xu J, Gonzalez ET, Iyer SS, Mac V, Mora AL, Sutliff RL. Use of senescence-accelerated mouse model in bleomycin-induced lung injury suggests that bone marrow-derived cells can alter the outcome of lung injury in aged mice.. J Gerontol A Biol Sci Med Sci 2009;64:731-9.
  120. Naik PN, Horowitz JC, Moore TA, Wilke CA, Toews GB, Moore BB. Pulmonary fibrosis induced by gamma-herpesvirus in aged mice is associated with increased fibroblast responsiveness to transforming growth factor-beta.. J Gerontol A Biol Sci Med Sci 2012;67:714-25.
  121. Accardi G, Caruso C. Immune-inflammatory responses in the elderly: an update.. Immun Ageing 2018;15:11.
  122. Adams AA, Breathnach CC, Katepalli MP, Kohler K, Horohov DW. Advanced age in horses affects divisional history of T cells and inflammatory cytokine production.. Mech Ageing Dev 2008;129:656-64.
  123. Wilkins PA. Equine multinodular pulmonary fibrosis.. Equine Vet Educ 2013;25(8):393-7.
  124. Geraminejad MD, Memar O, Aronson I, Rady PL, Hengge U, Tyring SK. Kaposi’s sarcoma and other manifestations of human herpesvirus 8.. J Am Acad Dermatol 2002;47(5):641-55.
  125. Boshoff C, Chang Y. Kaposi’s sarcoma-associated herpesvirus: a new DNA tumor virus.. Annu Rev Med 2001;52:453-70.
  126. Burkitt DP. Etiology of Burkitt's lymphoma- an alternative hypothesis to a vectored virus.. J Natl Cancer Inst 1969;42:19-28.
  127. Luppi M, Trovato R, Barozzi P, Vallisa D, Rossi G, Re A. Treatment of herpesvirus associated primary effusion lymphoma with intracavitary cidofovir.. Leukemia 2005;19:473-6.
  128. Halfdanarson TR, Markovic SN, Kalokhe U, Luppi M. A non-chemotherapy treatment of a primary effusion lymphoma: durable remission after intracavitary cidofovir I HIV negative PEL refractory to chemotherapy.. Ann Oncol 2006;17(12):1849-50.
  129. Stingaciu S, Ticchioni M, Sudaka I, Haudebourg J, Mounier N. Intracavitary cidofovir for human herpes virus 8 associated primary effusion lymphoma in an HIV-negative patient.. Clin Adv Hematol Oncol 2010;8(5):367-74.
  130. Rushton JO, Kolodziejek J, Nell B, Nowotny N. Prevalence of asinine herpesvirus type 5 (AsHV-5) infection in clinically normal Lipizzaner horses.. Vet J 2014;200(1):200-3.
  131. Scheurer L, Bachofen C, Herteman N, Hilbe M, Wolfer N, Schoster A. A case series highlighting the role of different gamma-herpesviruses in Equine Multinodular Pulmonary Fibrosis.. Schweizer Archiv für Tierheilkunde 2020;162(4):245-56.
  132. Bell SA, Pusterla N, Udeni BUBR, Mapes SM, Nyberg NL, MacLachlan NJ. Isolation of a gammaherpesvirus similar to asinine herpesvirus-2 (AHV-2) from a mule and a survey of mules and donkeys for AHV-2 infection by real-time PCR.. Vet Microbiol 2008;130:176-83.
  133. Jerele S, Davis E, Mapes S, Pusterla N, Navas Gonzalez FJ, Iglesias Pastrana C. Survey of serum amyloid A and bacterial and viral frequency using qPCR levels in recently captured feral donkeys from Death Valley National Park (California).. Animals 2020;10(6):1086.
  134. Barrandeguy ME, Carossino M. Infectious diseases in donkeys and mules: an overview and update.. J Equine Vet Sci 2018;65:98-105.
  135. LeCuyer TE, Rink A, Bradway DS, Evermann JF, Nicola AV, Baszler T. Abortion in a Mediterranean miniature donkey (Equus asinus) associated with a gammaherpesvirus similar to Equid herpesvirus 7.. J Vet Diagn Invest 2015;27:749-53.
  136. Mendoza FJ, Toribio RE, Perez-Ejica A. Donkey internal medicine-part II: cardiovascular, respiratory, neurologic, urinary, ophthalmic, dermatology and musculoskeletal disorders.. J Equine Vet Sci 2018;65:86-97.
  137. Rickards KJ, Thiemann AK. Respiratory disorders of the donkey.. Vet Clin North Am Equine Pract 2019;35:561-73.
  138. Miele A, Dhaliwal K, Du Toit N, Murchison JT, Dhaliwal C, Brooks H. Chronic pleuropulmonary fibrosis and elastosis of aged donkeys: similarities to human pleuroparenchymal fibroelastosis.. Chest 2014;145(6):1325-32.
  139. Vengust M, Wen X, Bienzle D. Herpesvirus-associated neurological disease in a donkey.. J Vet Diagn Invest 2008;20:820-3.
  140. Du Toit N, Genovese LM, Dalziel RG, Smith SH. Pulmonary angiocentric lymphoma (Lymphomatoid granulomatosis) in a donkey.. J Comp Pathol 2012;146(1):24-9.
  141. Back H, Kendall A, Grandón R, Ullman K, Treiberg-Berndtsson L, Ståhl K. Equine multinodular pulmonary fibrosis in association with asinine herpesvirus type 5 and equine herpesvirus type 5: a case report.. Acta Vet Scand 2012;54:57.
  142. Gomez De Witte FG, Frank N, Wilkes RP, Novak JM. Association of asinine herpesvirus-5 with pyogranulomatous pneumonia in a mare.. J Vet Intern Med 2012;26(4):1064-8.
  143. Pennington MR, Cossic BGA, Perkins GA, Duffy C, Duhamel GE, Van de Walle GR. First demonstration of equid gammaherpesviruses within the gastric mucosal epithelium of horses.. Virus Res 2017;242:30-6.
  144. Marenzoni ML, Bietta A, Lepri E, Casagrande Proietti P, Cordioli P, Canelli E. Role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality.. Vet Res Commun 2013;37:311-7.
  145. Lee SK, Lee I. The molecular detection of equine herpesviruses 2 and 5 in genital swabs from clinically normal Thoroughbred Mares in South Korea.. J Equine Vet Sci 2019;79:68-72.
  146. Kemker BP, Docherty JJ, De Lucia A, Ruf W, Lewis RD. Herpes simplex virus: a possible etiologic agent in some gastroduodenal ulcer disease.. Am Surg 1992;58:775-8.
  147. Archimandritis A, Markoulatos P, Tjivras M, Alexiou A, Kordossi A, Kordossis T. Herpes simplex viru types 1 and 2 and cytomegalovirus in peptic ulcer disease and non-ulcer dyspepsia.. Hepatogastroenterol 1992;39:540-1.
  148. Buzás GM, Konderák J. Co-infection with Helicobacter and Epstein-Barr virus in benign upper digestive diseases: an endoscopic and serologic pilot study.. United Eur Gastroenterol J 2016;4:388-94.
  149. Cárdenas-Mondrágon MG, Torres J, Flores-Luna L, Carreón-Talavera R, Camorlinga-Ponce M, Fuentes-Pananá EM. Epstein Barr virus gastritis.. Am J Surg Pathol 2007;31:1446-51.
  150. Galosi CM, de la Paz VC, Fernández LC, Martinez JP, Craig MI, Barrandeguy M. Isolation of equine herpesvirus-2 from the lung of an aborted fetus.. J Vet Diagn Invest 2005;17:500-2.
  151. Léon A, Fortier G, Fortier C, Freymuth F, Tapprest J, Leclercq R. Detection of equine herpesviruses in aborted foetuses by consensus PCR.. Vet Microbiol 2008;126:20-9.
  152. Marenzoni ML, Sforna M, Stefanetti V, Casagrande Proietti P, Brignone L, Del Sero A. Detection of Equid herpesvirus type 2 and 5 DNA in uterine flushings of mares with reproductive disorders.. Vet Microbiol 2014;174:570-6.
  153. Patterson-Kane JC, Donahue JM, Harrison LR. Placentitis, fetal pneumonia, and abortion due to Rhodococcus equi infection in a Thoroughbred.. J Vet Diagn Invest 2002;14:157-9.
  154. Agius CT, Crabb BS, Telford EA, Davison AJ, Studdert MJ. Comparative studies of the structural proteins and glycoproteins of equine herpesviruses 2 and 5.. J Gen Virol 1994;75(10):2707-17.
  155. Dunowska M, Howe L, Hanlon D, Stevenson M. Kinetics of Equid herpesvirus type 2 infections in a group of Thoroughbred foals.. Vet Microbiol 2011;152(1-2):176-80.
  156. Marenzoni ML, Passamonti F, Lepri E, Cercone M, Capomaccio S, Cappelli K. Quantification of Equid herpesvirus 5 DNA in clinical and necropsy specimens collected from a horse with equine multinodular pulmonary fibrosis.. J Vet Diagn Invest 2011;23(4):802-6.
  157. Hue ES, Fortier CI, Laurent AM, Quesnelle YF, Fortier GD, Legrand LJ. Development and validation of a quantitative PCR method for equid herpesvirus-2 diagnostics in respiratory fluids.. J Vis Exp 2016;109:53672.
  158. Thorsteinsdóttir L, Torsteinsdóttir S, Svansson V. Establishment and characterization of fetal equine kidney and lung cells with extended lifespan. Susceptibility to equine gammaherpesvirus infection and transfection efficiency.. In Vitro Cell Dev Biol-Animal 2016;52:872-7.
  159. Maxwell LK, Bentz BG, Gilliam LL, Ritchey JW, Pusterla N. Efficacy of the early administration of valacyclovir for the therapy of neuropathogenic EHV-1 in horses.. Amer J Vet Res 2017;78:1126-39.
  160. Garré B, van der Meulen K, Nugent J. In vitro susceptibility of six isolates of equine herpesvirus 1 to acyclovir, ganciclovir, cidofovir, adefovir, PMEDAP and foscarnet.. Vet Microbiol 2007;122(1-2):43-51.
  161. Wilkins PA, Papich M, Sweeney RW. Pharmacokinetics of acyclovir in adult horses.. J Vet Emerg Crit Care 2005;15:174-8.
  162. Bentz BG, Maxwell LK, Erkert RS, Royer CM, Davis MS, MacAllister CG. Pharmacokinetics of acyclovir after single intravenous and oral administration to adult horses.. J Vet Intern Med 2006;20:589-94.
  163. Garré B, Shebany K, Gryspeerdt A, Baert K, van der Meulen K, Nauwynck H. Pharmacokinetics of acyclovir after intravenous infusion of acyclovir and after oral administration of acyclovir and its prodrug valacyclovir in healthy adult horses.. Antimicrob Agents Chemother 2007;51:4308-14.
  164. Schwarz B, Schwendenwein I, van den Hoven R. Successful outcome in a case of Equine Multinodular Pulmonary Fibrosis (EMPF) treated with valacyclovir.. Equine Vet Educ 2013;25(8):389-92.
  165. Wong DM, Maxwell LK, Wilkins PA. Use of antiviral medications against equine herpes virus associated disorders.. Equine Vet Educ 2010;22(5):244-52.
  166. Easton-Jones CA, Madigan JE, Barnum A, Maxwell LK, Taylor SD, Arnesen T. Effect of valacyclovir on EHV-5 viral kinetics in horses with equine multinodular pulmonary fibrosis.. J Vet Internal Med 2018;32(5):1763-7.
  167. Hoshino Y, Katano H, Zou P, Hohman P, Marques A, Tyring SK. Long-term administration of valacyclovir reduces the number of Epstein-Barr virus (EBV)-infected B cells but not the number of EBV DNA copies per B cell in healthy volunteers.. J Virol 2009;83(22):11857-61.
  168. Maxwell LK. Antiherpetic drugs in equine medicine.. Vet Clin Equine 2017;33:99-125.
  169. Slater J. Equine herpesviruses.. 2007. In: Sellon DC, Long MT, editors. Equine infectious diseases. St Louis, MO: Saunders Elsevier; p. 134-53.
  170. Browning GF, Ficorilli N, Studdert MJ. Asinine herpesvirus genomes: comparison with those of the equine herpesvirus.. Arch Virol 1988;101:183-90.
  171. Wilcox GE. Latent equine herpesvirus: role of latent equine herpesviruses in respiratory disease.. 2004. Barton, MI: Rural industries research and development corporation.
  172. Pusterla N, Hussey SB, Mapes S, Johnson C, Collier JR, Hill J. Molecular investigation of the viral kinetics of equine herpesvirus-1 in blood and nasal secretions of horses after corticosteroid-induced recrudescence of latent infection.. J Vet Intern Med 2010;24:1153-7.
  173. Edington N, Bridges CG, Huckle A. Experimental reactivation of equid herpesvirus 1 (EHV 1) following the administration of corticosteroids.. Equine Vet J 1985;17:369-72.
  174. Slater JD, Borchers K, Thackray AM, Field HJ. The trigeminal ganglion is a location for equine herpesvirus 1 latency and reactivation in the horse.. J Gen Virol 1994;75:2007-16.
  175. Smith DJ, Iqbal J, Purewal A, Hamblin AS, Edington N. In vitro reactivation of latent equid herpesvirus-1 from CD5+/CD8+ leukocytes indirectly by IL-2 or chorionic gonadotrophin.. J Gen Virol 1998;79:2997-3004.
  176. Murata T. Regulation of Epstein Barr Virus reactivation from latency.. Microbiol Immunol 2014;58:307-17.
  177. Muscat KE, Padalino B, Hartley CA, Ficorilli N, Cell P, Knight P. Equine transport and changes in equid herpesvirus' status.. Front Vet Sci 2018;5:224.
  178. Shannon-Lowe C, Neuhierl B, Baldwin G, Rickinson A, Delecluse HJ. Resting B cells as a transfer vehicle for Epstein-Barr virus infection of epithelial cells.. Proc Natl Acad Sci USA 2006;103:7065-70.

Citations

This article has been cited 5 times.
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    doi: 10.3390/pathogens12020179pubmed: 36839451google scholar: lookup
  2. Acevedo HD, Hassebroek AM, Leventhal HR, Duhamel GE, Carvallo FR. Colonic T-cell-rich, large B-cell lymphoma associated with equid herpesvirus 5 infection and secondary trans-colonic fistula in a horse. J Vet Diagn Invest 2023 May;35(3):272-277.
    doi: 10.1177/10406387231155414pubmed: 36786313google scholar: lookup
  3. Badr C, Souiai O, Arbi M, El Behi I, Essaied MS, Khosrof I, Benkahla A, Chabchoub A, Ghram A. Epidemiological and Phylogeographic Study of Equid Herpesviruses in Tunisia. Pathogens 2022 Sep 5;11(9).
    doi: 10.3390/pathogens11091016pubmed: 36145448google scholar: lookup
  4. Charbonnel A, Lavoie JP, Juette T, St-Sauveur VG, Denis S, Gagnon CA, Leclère M. Comparison of nasal swabs and handmade foam cubes for detecting equine herpesvirus 5 (EHV-5) by quantitative polymerase chain reaction (qPCR). Can J Vet Res 2025 Jan;89(1):11-16.
    pubmed: 39744469
  5. Worku A, Molla W, Kenubih A, Negussie H, Admassu B, Ejo M, Dagnaw GG, Bitew AB, Fentahun T, Getnet K, Dejene H, Berrie K, Ibrahim SM, Gessese AT, Dessalegn B, Birhan M, Fenta MD, Kinde MZ. Molecular Detection of Equine Herpesviruses from Field Outbreaks in Donkeys in Northwest Amhara Region, Ethiopia. Vet Med Int 2024;2024:9928835.
    doi: 10.1155/2024/9928835pubmed: 39380749google scholar: lookup
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