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Recovery of Arcobacter spp. from nonlivestock species.

Abstract: The genus Arcobacter encompasses campylobacter-like organisms that grow in air at 25 degrees C. Arcobacter has been detected or isolated from clinically healthy livestock as well as aborted fetuses and has been presumptively identified as either Campylobacter or Leptospira, based on its growth in selective semisolid media. Because reports from nonlivestock species are limited, this study examined nine presumptive isolates of Arcobacter spp. from an alpaca (Vicugna pacos), black rhinoceros (Diceros bicornis), white rhinoceros (Ceratotherium simum), gorilla (Troglodytes gorilla), gazelle (Eudorcas thomsoni), rhea (Rhea americana), and aborted equine fetuses. Seven of these nine phenotypically identified isolates of Arcobacter were confirmed by a multiplex polymerase chain reaction assay. The remaining two isolates were subsequently identified as Arcobacter skirrowii (Case 5) and Campylobacter jejuni (Case 6) by sequence analysis of a 527-base pair fragment of the 16S rRNA gene. Together, these cases underscore the challenges to a clinical laboratory of identifying Arcobacter in cases which mimic vibrionic abortion or leptospirosis.
Publication Date: 2012-09-07 PubMed ID: 22950328DOI: 10.1638/2010-0194.1Google Scholar: Lookup
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  • Journal Article

Summary

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This research explores the presence of the organism Arcobacter in non-livestock species. The study confirmed that seven out of nine species had Arcobacter, and indicated the struggles in identifying it in cases resembling certain types of abortion or leptospirosis.

About Arcobacter

Arcobacter is a genus of bacteria resembling Campylobacter, with the ability to grow in air at 25 degrees Celsius. These organisms have been detected and isolated from healthy livestock as well as aborted fetuses. They are often misidentified as either Campylobacter or Leptospira because of their growth patterns in selective semisolid media.

Purpose of the Study

  • The research was conducted due to the limited reports on Arcobacter from non-livestock species.
  • The goal of the study was to effectively identify Arcobacter spp. in non-livestock species.

Study Method

Nine different non-livestock species were examined for the presence of Arcobacter. These included Alpaca, Black Rhinoceros, White Rhinoceros, Gorilla, Gazelle, Rhea, and aborted equine fetuses.

Study Findings

A multiplex polymerase chain reaction assay was used to confirm the presence of Arcobacter in seven out of the nine species. The remaining two samples were identified as Arcobacter skirrowii and Campylobacter jejuni, through sequence analysis of a 527-base pair segment of the 16S rRNA gene.

Conclusion

The findings from this research highlight the challenge of identifying Arcobacter in cases which mimic certain types of abortion or leptospirosis. This suggests a need for further studies and enhanced techniques to correctly identify and distinguish Arcobacter from other similar organisms, to prevent possible diagnostic mistakes.

Cite This Article

APA
Wesley IV, Schroeder-Tucker L. (2012). Recovery of Arcobacter spp. from nonlivestock species. J Zoo Wildl Med, 42(3), 508-512. https://doi.org/10.1638/2010-0194.1

Publication

ISSN: 1042-7260
NlmUniqueID: 8915208
Country: United States
Language: English
Volume: 42
Issue: 3
Pages: 508-512

Researcher Affiliations

Wesley, Irene V
  • Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, U.S. Department of Agriculture, Agricultural Research Service, Ames, Iowa 50010, USA. irene.wesley@ars.usda.gov
Schroeder-Tucker, Linda

    MeSH Terms

    • Aborted Fetus / microbiology
    • Abortion, Veterinary
    • Animals
    • Animals, Zoo
    • Antelopes
    • Arcobacter / isolation & purification
    • Camelids, New World
    • Female
    • Gorilla gorilla
    • Gram-Negative Bacterial Infections / microbiology
    • Gram-Negative Bacterial Infections / veterinary
    • Horse Diseases / microbiology
    • Horses
    • Perissodactyla
    • Pregnancy
    • Rheiformes

    Citations

    This article has been cited 1 times.
    1. Di Blasio A, Traversa A, Giacometti F, Chiesa F, Piva S, Decastelli L, Dondo A, Gallina S, Zoppi S. Isolation of Arcobacter species and other neglected opportunistic agents from aborted bovine and caprine fetuses. BMC Vet Res 2019 Jul 24;15(1):257.
      doi: 10.1186/s12917-019-2009-3pubmed: 31340816google scholar: lookup