Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection.
Abstract: Oocytes may be collected from live mares from either the stimulated preovulatory follicle or from all visible immature follicles. We evaluated the yield of mature oocytes, and of blastocysts after intracytoplasmic sperm injection (ICSI), for both follicle types. In Experiment 1, mares were assigned to Progesterone (1.2g biorelease progesterone weekly) or Control treatments. Transvaginal aspiration of all follicles was performed every 14 d. Overall, 596 follicles were aspirated, with a 54% oocyte recovery rate. There was no difference between treatments in number of follicles punctured (9.0 to 9.1) or oocytes recovered (4.8 to 5.0) per mare per aspiration session. Of 314 oocytes recovered, 180 (57%) matured in culture. Thirty-six mature oocytes were subjected to ICSI; 33% formed blastocysts (63% per mare per aspiration session). In Experiment 2, the preovulatory follicle was aspirated every 14 d for three to four cycles. Prostaglandin F(2 alpha) was given on Days 6 and 7 after aspiration. A follicle >or=25 mm in diameter was present on Day 13, the day of deslorelin administration, in 23 of 24 cycles, and ovulatory response (granulosa expansion) was seen in 24 of 25 follicles aspirated. Blastocyst development after ICSI was 41% per injected oocyte, or an estimated 33% per mare per aspiration session. We concluded that both aspiration of immature follicles and aspiration of the preovulatory follicle can be performed effectively every 14 d without monitoring ovarian follicular growth. As performed in these separate experiments, aspiration of immature follicles provided more blastocysts per aspiration session.
(c) 2010 Elsevier Inc. All rights reserved.
Publication Date: 2010-03-04 PubMed ID: 20202674DOI: 10.1016/j.theriogenology.2010.01.013Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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The research article involves two experiments investigating the efficiency of harvesting oocytes from mares on a regular two-week schedule, and the subsequent development of these oocytes into blastocysts after intracytoplasmic sperm injection (ICSI). They found that using this method, both mature and preovulatory follicles could be effectively aspirated, providing more blastocysts per session.
Objective of the Research
- The primary aim of this study was to evaluate the effectiveness of a regular oocyte retrieval schedule in mares, and to ascertain the resulted blastocyst formation post intracytoplasmic sperm injection (ICSI) on the matured oocytes.
Research Methodology
- Two experiments were carried out as part of the study. In the first experiment, mares were subjected to one of two treatments: a control treatment or a weekly progesterone treatment. Every 14 days, the mares underwent transvaginal follicle aspiration and the oocytes were assessed. There was a total aspiration of 596 follicles with a recovery rate of 54%.
- The result was observed by the number of follicles punctured and the oocytes that were recovered per mare for each session of aspiration.
- Of the recovered oocytes, 57% matured in culture and around 33% of these matured oocytes developed into blastocysts after ICSI was executed. The blastocyst formation rate per mare per aspiration session was approximately 63%.
- In the second experiment, the preovulatory follicle was aspirated every 14 days for three to four cycles. A follicle larger than or equal to 25 mm in diameter was always found to be present on the 13th day, which was the day of deslorelin administration. This led to an ovulatory response.
- The rate of blastocyst development after ICSI was around 41% per injected oocyte, or an estimated 33% per mare per aspiration session.
Conclusions of the Research
- The study concluded that the aspiration process of both immature follicles and the preovulatory follicle can be effectively carried out every 14 days without the need for monitoring the growth of ovarian follicles.
- The research demonstrated that this method resulted in more blastocysts each time the aspiration session was performed, when compared to current methods.
Cite This Article
APA
Jacobson CC, Choi YH, Hayden SS, Hinrichs K.
(2010).
Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection.
Theriogenology, 73(8), 1116-1126.
https://doi.org/10.1016/j.theriogenology.2010.01.013 Publication
Researcher Affiliations
- Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843-4466, USA.
MeSH Terms
- Animals
- Blastocyst / cytology
- Blastocyst / physiology
- Cell Count
- Estrous Cycle / blood
- Estrous Cycle / physiology
- Female
- Horses
- Male
- Oocyte Retrieval / methods
- Oocyte Retrieval / veterinary
- Oocytes / cytology
- Oocytes / physiology
- Ovarian Follicle / cytology
- Ovulation Induction / methods
- Pregnancy
- Progesterone / blood
- Sperm Injections, Intracytoplasmic
- Time Factors
- Treatment Outcome
Citations
This article has been cited 14 times.- Van den Branden E, Salamone M, Broothaers K, Peere S, Polfliet E, Dewulf M, Van Steenkiste G, van Loon G, Smits K, Govaere J. Physiological and behavioral parameters of pain and stress in mares during and after transvaginal ultrasound-guided follicular aspiration. Front Vet Sci 2025;12:1574351.
- Felix MR, Dobbie T, Woodward E, Linardi R, Okada C, Santos R, Hinrichs K. Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes. Biol Reprod 2025 May 13;112(5):867-879.
- Teng M, Zhao M, Mu B, Lei A. Allogenic Follicular Fosterage Technology: Problems, Progress and Potential. Vet Sci 2024 Jun 17;11(6).
- Luis-Calero M, Ortiz-Rodríguez JM, Fernández-Hernández P, Muñoz-García CC, Pericuesta E, Gutiérrez-Adán A, Marinaro F, Embade N, Conde R, Bizkarguenaga M, Millet Ó, González-Fernández L, Macías-García B. Preovulatory follicular fluid secretome added to in vitro maturation medium influences the metabolism of equine cumulus-oocyte complexes. BMC Vet Res 2024 Jun 25;20(1):272.
- de la Fuente A, Scoggin C, Bradecamp E, Martin-Pelaez S, van Heule M, Troedsson M, Daels P, Meyers S, Dini P. Transcriptome Signature of Immature and In Vitro-Matured Equine Cumulus-Oocytes Complex. Int J Mol Sci 2023 Sep 6;24(18).
- Huijsmans TERG, Hassan HA, Smits K, Van Soom A. Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art. Animals (Basel) 2023 Apr 15;13(8).
- Biasetti P, Hildebrandt TB, Göritz F, Hermes R, Holtze S, Galli C, Lazzari G, Colleoni S, Pollastri I, Spiriti MM, Stejskal J, Seet S, Zwilling J, Ngulu S, Mutisya S, Kariuki L, Lokolool I, Omondo P, Ndeereh D, de Mori B. Ethical Analysis of the Application of Assisted Reproduction Technologies in Biodiversity Conservation and the Case of White Rhinoceros (Ceratotherium simum) Ovum Pick-Up Procedures. Front Vet Sci 2022;9:831675.
- Benammar A, Derisoud E, Vialard F, Palmer E, Ayoubi JM, Poulain M, Chavatte-Palmer P. The Mare: A Pertinent Model for Human Assisted Reproductive Technologies?. Animals (Basel) 2021 Aug 4;11(8).
- Kaps M, Okada CTC, Gautier CM, Aurich J, Aurich C. Deslorelin Slow-Release Implants Delay Ovulation and Increase Plasma AMH Concentration and Small Antral Follicles in Haflinger Mares. Animals (Basel) 2021 May 28;11(6).
- Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet 2019 Jun;36(6):1237-1250.
- Grady ST, Watts AE, Thompson JA, Penedo MCT, Konganti K, Hinrichs K. Effect of intra-ovarian injection of mesenchymal stem cells in aged mares. J Assist Reprod Genet 2019 Mar;36(3):543-556.
- Salgado RM, Brom-de-Luna JG, Resende HL, Canesin HS, Hinrichs K. Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation. J Assist Reprod Genet 2018 May;35(5):825-840.
- Lee W, Song K, Lee I, Shin H, Lee BC, Yeon S, Jang G. Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system. J Vet Sci 2015;16(4):509-16.
- Martino NA, Dell'Aquila ME, Filioli Uranio M, Rutigliano L, Nicassio M, Lacalandra GM, Hinrichs K. Effect of holding equine oocytes in meiosis inhibitor-free medium before in vitro maturation and of holding temperature on meiotic suppression and mitochondrial energy/redox potential. Reprod Biol Endocrinol 2014 Oct 11;12:99.
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