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Home / Equine Research Bank / Tissue Eng Part A / Regenerative medicine approach to reconstruction of the equi...
Tissue engineering. Part A2014; 20(7-8); 1213-1221; doi: 10.1089/ten.TEA.2013.0217

Regenerative medicine approach to reconstruction of the equine upper airway.

Abstract: Airway obstruction is a common cause of poor performance in horses. Structural abnormalities (insufficient length, rigidity) can be a cause for the obstruction. Currently, there are a few effective clinical options for reconstruction of the equine larynx. A regenerative medicine approach to reconstruction may provide the capability to stabilize laryngeal structures and to encourage restoration of site-appropriate, functional, and host-derived tissue. The purpose of this study was the histopathological evaluation of (1) decellularization of equine (horse) laryngeal cartilages (epiglottis and arytenoids); (2) the host response to decellularized laryngeal cartilages implanted subcutaneously in a donkey model as a test of biocompatibility; and (3) the use of decellularized laryngeal cartilages in a clinically relevant pilot study in the horse larynx. Equine laryngeal cartilages were found to be sufficiently decellularized and were subsequently implanted subcutaneously in donkeys to test biocompatibility. After 4 weeks, the implanted cartilage was harvested. In the subcutaneous model, the samples did not elicit a rejection or foreign body type reaction and were judged suitable for implantation in a clinically relevant equine model. Implants were placed in the upper airway (arytenoids and epiglottis) of one horse. At 4 weeks, the implants were observed to remodel rapidly and were replaced by dense connective tissue with signs of new hyaline cartilage formation in the arytenoids and by connective tissue containing glandular structures and an epithelial covering in the epiglottis. The results of the present study demonstrate the feasibility of a scaffold-based regenerative medicine approach to reconstruction of the equine upper airway; however, further studies investigating long-term integration, formation of new cartilage, and mechanical properties are needed.
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Publication Date: 2014-03-21 PubMed ID: 24160675PubMed Central: PMC3993024DOI: 10.1089/ten.TEA.2013.0217Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores a regenerative medicine technique for treating airway obstruction in horses. It involves the use of decellularized laryngeal cartilages implanted in horses that can potentially lead to stabilizing laryngeal structures and encourage reformation of functional tissues.

Research Objective and Methodology

  • The study aimed to examine a regenerative medicine approach to reconstructing the equine upper airway. This research was primarily focused on using decellularized horse laryngeal cartilages (from the epiglottis and arytenoids) as implants in a clinical setting.
  • The first step of this investigation involved the successful decellularization of the aforementioned equine laryngeal cartilages.
  • The decellularization is an essential procedure as it ensures the removal of all cellular constituents that could cause a potential immune response, leaving behind only the structural proteins which act as a scaffold for new tissue growth.
  • The decellularized tissues were then implanted subcutaneously in donkeys, serving as a model to test the biocompatibility of these tissues.

Observations and Findings

  • After four weeks of implanting the decellularized tissues in the donkey model, the researchers harvested the implanted cartilage for evaluation.
  • No instances of rejection or foreign body type reactions were observed in these implanted samples, thus indicating a successful biocompatibility test.
  • Following these positive results, the decellularized laryngeal cartilages were then implanted in actual clinical conditions in the upper airway (arytenoids and epiglottis) of a horse.
  • After four weeks, promising signs of tissue regeneration were seen. The implants were found to remodel rapidly, replaced by dense connective tissue which showed indications of new hyaline cartilage formation in the arytenoids and connective tissue containing glandular structures and an epithelial covering in the epiglottis.

Conclusion and Further Study

  • From these observations, the researchers concluded that the scaffold-based regenerative medicine approach shows promise for reconstructing the equine upper airway.
  • It was, however, also noted that further studies investigating long-term integration, formation of new cartilage, and mechanical properties are necessary before this technique can be widely used in clinical practice.

Cite This Article

APA
Grevemeyer B, Bogdanovic L, Canton S, St Jean G, Cercone M, Ducharme NG, Brown BN. (2014). Regenerative medicine approach to reconstruction of the equine upper airway. Tissue Eng Part A, 20(7-8), 1213-1221. https://doi.org/10.1089/ten.TEA.2013.0217

Publication

Tissue engineering. Part A
ISSN: 1937-335X
NlmUniqueID: 101466659
Country: United States
Language: English
Volume: 20
Issue: 7-8
Pages: 1213-1221

Researcher Affiliations

Grevemeyer, Bernard
  • 1 Department of Clinical Sciences, Ross University , School of Veterinary Medicine, Basseterre, St.Kitts.
Bogdanovic, Lewis
    Canton, Stephen
      St Jean, Guy
        Cercone, Marta
          Ducharme, Norm G
            Brown, Bryan N

              MeSH Terms

              • Animals
              • Arytenoid Cartilage / transplantation
              • Endoscopy
              • Epiglottis / cytology
              • Epiglottis / physiology
              • Equidae
              • Freeze Drying
              • Horses
              • Implants, Experimental
              • Larynx / physiology
              • Larynx / surgery
              • Prosthesis Implantation
              • Plastic Surgery Procedures
              • Regenerative Medicine / methods
              • Subcutaneous Tissue

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              Citations

              This article has been cited 7 times.
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