Regional distribution and integrity of equine ovarian pre-antral follicles.
Abstract: The goal of this study was to determine the distribution of pre-antral follicles in the ovarian parenchyma of mares. For Experiment 1, each ovary was cut longitudinally at the greater curvature, performing two hemiovaries. After that, six fragments from each hemiovary were obtained, resulting in 12 fragments, which were divided into the innermost region of the parenchyma, the middle region and the outermost region. All the three obtained sections were cut transversally to obtain two fragments from each one. For Experiment 2, each ovary also submitted to a longitudinal cut on the greater curvature, forming two hemiovaries. Each hemiovary was sectioned into four symmetrical fragments, resulting in eight fragments per ovary. The fragments were related as being near to or far from the ovulatory fossa. The fragments of both experiments were immediately fixed in Carnoy for 12 hr and kept in 70% ethanol for 24 hr. Follicles were classified according to the stages of development and for morphological integrity according to oocyte morphology and granulosa cells. After the histological assessment, a total of 1,130 follicles were visualized from Experiment 1, being 1,054 (93.3%) primordial follicles and 76 (4.7%) follicles in development. The innermost region had the highest percentage of pre-antral follicles compared to the other regions (p < .05). The middle and outermost regions showed higher percentages of intact primordial and developing follicles than the innermost region (p < .05). Considering Experiment 2, 938 follicles were found, being 894 (95.3%) primordial and 44 (4.7%) follicles in development. The region near the ovulatory fossa presented higher (58.7%; 551 of 938) follicular concentration compared to the region far from the ovulatory fossa (41.3%; 387 of 938; p < .05). As a conclusion, distribution of pre-antral follicles in the equine ovary has a specific pattern through the parenchyma. Also, the follicular integrity differed in the studied ovarian areas.
© 2017 Blackwell Verlag GmbH.
Publication Date: 2017-06-04 PubMed ID: 28580754DOI: 10.1111/rda.12986Google Scholar: Lookup
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- Journal Article
Summary
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The research article explores the distribution and structural integrity of pre-antral follicles within the ovarian parenchyma in horses, making use of two experiments employing different fragmenting methods and assessing follicular concentration.
Objective and Methodology
- The main aim of this study was to ascertain the distribution of pre-antral follicles within the ovarian parenchyma of mares, with a specific focus on the spatial pattern of these follicles within different regions of the ovary.
- The study used two distinct approach for this investigation which involved sectioning each ovary longitudinally along the greater curvature to form two hemiovaries, followed by further subdivision into fragments.
- In the first experiment, six fragments were extracted from each hemiovary, equating to a total of 12 per ovary. These were divided into three categories based on their location: innermost region, middle region, and outermost region.
- The second experiment also divided each ovary into two hemiovaries, though these were further divided into four symmetrical fragments each, resulting in a total of eight fragments per ovary. Fragments were classified as either near or far from the ovulatory fossa.
- All resultant fragments from both experiments were preserved using Carnoy solution followed by 70% ethanol, and were then assessed for follicular development and morphological integrity.
Findings
- From the first experiment, a total of 1,130 follicles were observed, 93.3% of which were classified as primordial while the remaining 4.7% were classified as in development.
- It was found that the innermost region contained the highest percentage of pre-antral follicles compared to other regions, while higher percentages of intact primordial and developing follicles were found in the middle and outermost regions.
- The second experiment showed similar proportions of primordial and developing follicles (95.3% and 4.7% respectively).
- The region closer to the ovulatory fossa contained a higher follicular concentration (58.7%) compared to the region further away (41.3%).
Conclusion
- The researchers concluded by highlighting the existence of a specific pattern regarding the distribution of pre-antral follicles within equine ovarian parenchyma. Furthermore, follicular integrity varied among different regions of the ovary.
Cite This Article
APA
Gonzalez SM, da Silva CB, Lindquist AG, Bufalo I, Morotti F, Lisboa LA, Seneda M.
(2017).
Regional distribution and integrity of equine ovarian pre-antral follicles.
Reprod Domest Anim, 52(5), 836-841.
https://doi.org/10.1111/rda.12986 Publication
Researcher Affiliations
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
- Laboratory of Animal Reproduction, University of Londrina, Parana, Brazil.
MeSH Terms
- Animals
- Female
- Granulosa Cells
- Horses
- Oocytes
- Ovarian Follicle / anatomy & histology
- Ovarian Follicle / physiology
- Ovary / anatomy & histology
Citations
This article has been cited 3 times.- Hyde KA, Aguiar FLN, Alves BG, Alves KA, Gastal GDA, Gastal MO, Gastal EL. Preantral follicle population and distribution in the horse ovary.. Reprod Fertil 2022 Apr 1;3(2):90-102.
- Max MC, Bizarro-Silva C, Búfalo I, González SM, Lindquist AG, Gomes RG, Barreiros TRR, Lisboa LA, Morotti F, Seneda MM. In vitro culture supplementation of EGF for improving the survival of equine preantral follicles.. In Vitro Cell Dev Biol Anim 2018 Dec;54(10):687-691.
- Alves BG, Alves KA, Gastal GDA, Gastal MO, Figueiredo JR, Gastal EL. Spatial distribution of preantral follicles in the equine ovary.. PLoS One 2018;13(6):e0198108.
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