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Home / Equine Research Bank / Reprod Domest Anim / Relationship between DNA fragmentation of equine granulosa c...
Reproduction in domestic animals = Zuchthygiene2019; 54 Suppl 4; 78-81; doi: 10.1111/rda.13514

Relationship between DNA fragmentation of equine granulosa cells and oocyte meiotic competence after in vitro maturation.

Abstract: The acquisition of equine oocyte developmental capacity is ensured by the follicular environment, such as granulosa cells, which could reflect the meiotic development potential of immature oocytes. This study evaluated the relationship between DNA fragmentation of granulosa cells, using the chromatin dispersion test, and equine oocyte meiotic development after in vitro maturation. Granulosa cells and cumulus-oocytes complexes (n = 50) were recovered from slaughterhouse-derived ovaries. Oocytes were in vitro matured, stained and evaluated under fluorescence microscopy. Maturation rates were classified into outstanding, medium and poor levels of maturation using 25th and 75th percentiles as thresholds. For DNA assessment, each sample was processed with the Ovoselect kit (Halotech DNA). High, low and total DNA fragmentation percentages were compared among levels of maturation rates by ANOVA, followed by Duncan test. Results were expressed as mean ± SE. Total and high DNA fragmentation rates of granulosa cells were significantly higher (p < 0.05) in follicles whose oocytes had reached outstanding maturation level than those originating from follicles whose oocytes had reached poor maturation level. In conclusion, the DNA fragmentation analysis of equine granulosa cells can be a valuable test to identify equine oocytes showing the best meiotic competence after in vitro maturation.
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-10-19 PubMed ID: 31625232DOI: 10.1111/rda.13514Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores the relationship between the DNA fragmentation of granulosa cells in horses and the meiotic development of oocytes post in vitro maturation, indicating that the DNA fragmentation analysis could help identify oocytes with better meiotic competence after in vitro maturation.

Research Objective and Process

  • The study looks into the correlation between the DNA fragmentation in equine granulosa cells and the maturation level of the oocytes after in vitro procedures.
  • Granulosa cells and cumulus-oocyte complexes were obtained from ovaries derived from a slaughterhouse.
  • Following in vitro maturation, the oocytes were stained and assessed under fluorescence microscopy.

Maturation Rates and DNA Evaluation

  • Maturation rates were divided into outstanding, medium, and poor levels, using the 25th and 75th percentiles as thresholds.
  • For each sample, the Ovoselect kit was used for the DNA fragmentation analysis.
  • The study then compared high, low and total DNA fragmentation percentages amongst different maturation rates, using ANOVA and the Duncan test.

Findings and Conclusion

  • The study found that total and high DNA fragmentation rates of granulosa cells were significantly higher in follicles with oocytes that had achieved an outstanding maturation level than those from follicles where oocytes had shown poor maturation.
  • Therefore, the study concluded that analyzing DNA fragmentation of equine granulosa cells could serve as a beneficial test to identify equine oocytes with the best meiotic capabilities post-in vitro maturation.

Cite This Article

APA
Pereira B, Dorado J, Diaz-Jimenez M, Consuegra C, Ortiz I, Gosalvez J, Hidalgo M. (2019). Relationship between DNA fragmentation of equine granulosa cells and oocyte meiotic competence after in vitro maturation. Reprod Domest Anim, 54 Suppl 4, 78-81. https://doi.org/10.1111/rda.13514

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 54 Suppl 4
Pages: 78-81

Researcher Affiliations

Pereira, Blasa
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.
Dorado, Jesús
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.
Diaz-Jimenez, Maria
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.
Consuegra, Cesar
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.
Ortiz, Isabel
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.
Gosalvez, Jaime
  • Department of Biology, Autonomous University of Madrid, Madrid, Spain.
Hidalgo, Manuel
  • Veterinary Reproduction Group, Department of Animal Medicine and Surgery, Veterinary Faculty, University of Cordoba, Cordoba, Spain.

MeSH Terms

  • Animals
  • DNA Fragmentation
  • Female
  • Granulosa Cells / physiology
  • Horses
  • In Vitro Oocyte Maturation Techniques / veterinary
  • Meiosis / physiology
  • Oocytes / physiology

Grant Funding

  • EJI-17-AGR-275 / Conserjeru00eda de Conocimiento, Investigaciu00f3n y Universidad (Junta de Andalucu00eda, Spain)
  • ES-F_2014-2020 / European Social Fund
  • AGL2013-42726-R / MINECO

References

This article includes 13 references
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Citations

This article has been cited 1 times.
  1. Ortiz I, Dorado J, Pereira B, Diaz-Jimenez M, Consuegra C, Gosalvez J, Hidalgo M. DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte. Reprod Domest Anim 2022 Oct;57 Suppl 5(Suppl 5):64-67.
    doi: 10.1111/rda.14197pubmed: 35770452google scholar: lookup
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