Relationship of cysteine-rich secretory protein-3 gene and protein with semen quality in stallions.
- Journal Article
Summary
The research article investigates the effect of the Cysteine-rich secretory protein-3 (CRISP-3) gene and protein on the quality of semen in stallions. The study uncovers a positive relationship between certain genotypes of the CRISP-3 gene and protein and better semen quality in these animals.
Article Overview
The research study aimed to understand the link between the CRISP-3 gene and protein and the quality of raw semen in stallions. Prior research has shown a connection between CRISP-3 and the fertility and freezability of stallion semen; however, the specific role of the gene and protein has not been fully explored.
Methodology
- The researchers collected DNA from blood samples of 100 stallions.
- 30 stallions were randomly selected from the group to provide 60 semen samples for the study.
- The team examined variations in four nonsynonymous SNPs from CRISP-3 using PCR amplification and sequencing methods.
- The quality of the semen was gauged against measures like total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI), and a seminal quality index (SQi).
- The ELISA technique was applied to determine the CRISP-3 protein content in the seminal plasma (SP).
Findings
- It was found that homozygous genotypes for SNP1, SNP2, and SNP3 and the heterozygous genotype for SNP4 displayed a positive impact on semen quality.
- Various haplotypes had a positive effect on MOT, SV, NM, MI, and SQi.
- The allelic substitution analysis indicated positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3).
- A higher concentration of CRISP-3 protein resulted in an elevated MOT and SQi.
Conclusion
The study concluded that the quality of stallion semen is affected by both the genotype of the CRISP-3 gene and the concentration of the respective protein in the seminal plasma. This research suggests that CRISP-3 could be a potential marker for predicting semen quality in stallions.
Cite This Article
Publication
Researcher Affiliations
- Department of Animal Science, Faculty of Agricultural Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
- School of Chemistry, Faculty of Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
- Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia.
MeSH Terms
- Animals
- Fertility
- Haplotypes
- Horses / genetics
- Male
- Polymorphism, Single Nucleotide
- Semen / chemistry
- Semen Analysis / veterinary
- Seminal Plasma Proteins / analysis
- Seminal Plasma Proteins / genetics
- Sperm Motility
- Spermatozoa / physiology
Citations
This article has been cited 7 times.- Ďuračka M, Benko F, Tvrdá E. Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.. Int J Mol Sci 2023 Feb 8;24(4).
- Zhang M, Bromfield EG, Helms JB, Gadella BM. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†.. Biol Reprod 2022 Nov 14;107(5):1242-1253.
- Nikitkina EV, Dementieva NV, Shcherbakov YS, Atroshchenko MM, Kudinov AA, Samoylov OI, Pozovnikova MV, Dysin AP, Krutikova AA, Musidray AA, Mitrofanova OV, Plemyashov KV, Griffin DK, Romanov MN. Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds.. Anim Biosci 2022 Dec;35(12):1827-1838.
- Harris EA, Stephens KK, Winuthayanon W. Extracellular Vesicles and the Oviduct Function.. Int J Mol Sci 2020 Nov 5;21(21).
- Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation.. Int J Mol Sci 2020 Sep 3;21(17).
- de Almeida Monteiro Melo Ferraz M, Nagashima JB, Noonan MJ, Crosier AE, Songsasen N. Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs.. Int J Mol Sci 2020 May 25;21(10).
- Johannisson A, Al-Essawe EM, Al-Saffar AK, Karkehabadi S, Lima-Verde I, Wulf M, Aurich C, Morrell JM. Season does not have a deleterious effect on proportions of stallion seminal plasma proteins.. J Reprod Dev 2020 Jun 12;66(3):215-221.