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Relationship of cysteine-rich secretory protein-3 gene and protein with semen quality in stallions.
Abstract: Cysteine-rich secretory protein-3 (CRISP-3) and some of its nonsynonymous polymorphism have been related to the fertility and freezability of stallion semen; however, the role of the CRISP-3 gene and its seminal plasma protein in the raw semen quality is still unknown. The aim of this study was to evaluate the relationship of CRISP-3 with semen quality in stallions. DNA was obtained from blood samples of 100 stallions, from which 30 stallions were randomly selected to obtain 60 ejaculates. Through PCR amplification and sequencing, the variation of four nonsynonymous SNPs from CRISP-3 was identified and haplotypes were derived. Semen quality was assessed through the total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI) and a seminal quality index (SQi). CRISP-3 protein content of seminal plasma (SP) was determined by ELISA. The effect of the genotype, the haplotype and the concentration of the CRISP-3 protein on the seminal quality were evaluated through generalized linear models and linear regression analyses. Homozygous genotypes for SNP1, SNP2 and SNP3 and the heterozygous genotype for SNP4 showed a positive effect on seminal quality. Different haplotypes with positive effect on MOT, SV, NM, MI and SQi were identified. The allelic substitution analysis resulted in positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3). A high level of CRISP-3 resulted in a higher MOT and SQi. It is concluded that the quality of stallion semen is influenced by the genotype of CRISP-3 and the concentration of CRISP-3 protein in SP.
© 2018 Blackwell Verlag GmbH.
Publication Date: 2018-09-19 PubMed ID: 30099787DOI: 10.1111/rda.13309Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article investigates the effect of the Cysteine-rich secretory protein-3 (CRISP-3) gene and protein on the quality of semen in stallions. The study uncovers a positive relationship between certain genotypes of the CRISP-3 gene and protein and better semen quality in these animals.
Article Overview
The research study aimed to understand the link between the CRISP-3 gene and protein and the quality of raw semen in stallions. Prior research has shown a connection between CRISP-3 and the fertility and freezability of stallion semen; however, the specific role of the gene and protein has not been fully explored.
Methodology
- The researchers collected DNA from blood samples of 100 stallions.
- 30 stallions were randomly selected from the group to provide 60 semen samples for the study.
- The team examined variations in four nonsynonymous SNPs from CRISP-3 using PCR amplification and sequencing methods.
- The quality of the semen was gauged against measures like total motility (MOT), sperm vitality (SV), normal morphology (NM), functional integrity of membrane (MI), and a seminal quality index (SQi).
- The ELISA technique was applied to determine the CRISP-3 protein content in the seminal plasma (SP).
Findings
- It was found that homozygous genotypes for SNP1, SNP2, and SNP3 and the heterozygous genotype for SNP4 displayed a positive impact on semen quality.
- Various haplotypes had a positive effect on MOT, SV, NM, MI, and SQi.
- The allelic substitution analysis indicated positive regression coefficients for MOT (SNP2) and MI (SNP2 and SNP3).
- A higher concentration of CRISP-3 protein resulted in an elevated MOT and SQi.
Conclusion
The study concluded that the quality of stallion semen is affected by both the genotype of the CRISP-3 gene and the concentration of the respective protein in the seminal plasma. This research suggests that CRISP-3 could be a potential marker for predicting semen quality in stallions.
Cite This Article
APA
Restrepo G, Rojano B, Usuga A.
(2018).
Relationship of cysteine-rich secretory protein-3 gene and protein with semen quality in stallions.
Reprod Domest Anim, 54(1), 39-45.
https://doi.org/10.1111/rda.13309 Publication
Researcher Affiliations
- Department of Animal Science, Faculty of Agricultural Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
- School of Chemistry, Faculty of Sciences, Universidad Nacional de Colombia, Medellín, Colombia.
- Faculty of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia.
MeSH Terms
- Animals
- Fertility
- Haplotypes
- Horses / genetics
- Male
- Polymorphism, Single Nucleotide
- Semen / chemistry
- Semen Analysis / veterinary
- Seminal Plasma Proteins / analysis
- Seminal Plasma Proteins / genetics
- Sperm Motility
- Spermatozoa / physiology
Citations
This article has been cited 11 times.- Ďuračka M, Benko F, Tvrdá E. Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability. Int J Mol Sci 2023 Feb 8;24(4).
- Zhang M, Bromfield EG, Helms JB, Gadella BM. The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization†. Biol Reprod 2022 Nov 14;107(5):1242-1253.
- Nikitkina EV, Dementieva NV, Shcherbakov YS, Atroshchenko MM, Kudinov AA, Samoylov OI, Pozovnikova MV, Dysin AP, Krutikova AA, Musidray AA, Mitrofanova OV, Plemyashov KV, Griffin DK, Romanov MN. Genome-wide association study for frozen-thawed sperm motility in stallions across various horse breeds. Anim Biosci 2022 Dec;35(12):1827-1838.
- Harris EA, Stephens KK, Winuthayanon W. Extracellular Vesicles and the Oviduct Function. Int J Mol Sci 2020 Nov 5;21(21).
- Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation. Int J Mol Sci 2020 Sep 3;21(17).
- de Almeida Monteiro Melo Ferraz M, Nagashima JB, Noonan MJ, Crosier AE, Songsasen N. Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs. Int J Mol Sci 2020 May 25;21(10).
- Johannisson A, Al-Essawe EM, Al-Saffar AK, Karkehabadi S, Lima-Verde I, Wulf M, Aurich C, Morrell JM. Season does not have a deleterious effect on proportions of stallion seminal plasma proteins. J Reprod Dev 2020 Jun 12;66(3):215-221.
- Ullah A, Chen W, Shi L, Wang M, Geng M, Na J, Akhtar MF, Khan MZ, Wang C. Challenges and Enhancing Strategies of Equine Semen Preservation: Nutritional and Genetic Perspectives. Vet Sci 2025 Aug 25;12(9).
- Khan MZ, Chen W, Naz S, Liu X, Liang H, Chen Y, Kou X, Liu Y, Ashraf I, Han Y, Peng Y, Wang C, Zahoor M. Determinant genetic markers of semen quality in livestock. Front Endocrinol (Lausanne) 2024;15:1456305.
- Sutovsky P, Hamilton LE, Zigo M, Ortiz D'Avila Assumpção ME, Jones A, Tirpak F, Agca Y, Kerns K, Sutovsky M. Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†. Biol Reprod 2024 Jun 12;110(6):1135-1156.
- Bu Y, Wang P, Li S, Li L, Zhang S, Wei H. Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation. Int J Mol Sci 2024 Feb 14;25(4).
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