Report of the International Equine Gene Mapping Workshop: male linkage map.
Abstract: The goal of the First International Equine Gene Mapping Workshop, held in 1995, was the construction of a low density, male linkage map for the horse. For this purpose, the International Horse Reference Family Panel (IHRFP) was established, consisting of 12 paternal half-sib families with 448 half-sib offspring provided by 10 laboratories. Blood samples were collected and DNA extracted in each laboratory and sent to the Lexington laboratory (KY, USA) for dispatch in aliquots to 14 typing laboratories. In total, 161 markers (144 microsatellites, seven blood groups and 10 proteins) were tested for all families for which the sire was heterozygous. Genealogies and typing data were sent for analysis to the INRA laboratory (Jouy-en-Josas, France) according to a specific format and entered into a database with input verification and output processes. Linkage analysis was performed with the CRIMAP program. Significant linkage was detected for 124 loci, of which 95 were unambiguously ordered using a multipoint analysis with an average spacing of 14.2 CM. These loci were distributed among 29 linkage groups. A more comprehensive analysis including synteny group data and FISH data suggested that 26 autosomes out of 31 are covered. The complete map spans 936 CM.
Publication Date: 1999-12-03 PubMed ID: 10582279DOI: 10.1046/j.1365-2052.1999.00510.xGoogle Scholar: Lookup
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- Congress
- Research Support
- Non-U.S. Gov't
Summary
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This research documents the creation of a genetic map for horses by a team of international scientists during the First International Equine Gene Mapping Workshop, using blood samples from a large family-based sample. The aim was to construct a base level, male lineage genetic map that covers majority of the horse chromosomes.
Research Objectives and Methodology
- The primary objective of this research was to construct a low-density, male linkage map for horses, which aims at mapping genes based on their relative positions and genetic recombination frequency.
- The methodology involved creating an International Horse Reference Family Panel (IHRFP), composed of 12 paternal half-sibling families that included 448 half-sibling offspring.
- All of the blood samples required for the study were collected and processed for DNA extraction in 10 different laboratories.
- Once the DNA was isolated, it was sent to a central laboratory in Lexington, KY, USA, where it was portioned out and shipped to 14 different gene typing laboratories.
Data Analysis Process
- A total of 161 markers, including 144 microsatellites, seven blood groups, and 10 proteins, were analyzed by testing all families.
- The marker data and associated genealogies were sent to the INRA laboratory in Jouy-en-Josas, France, for further analysis and database entering.
- Through a software program called CRIMAP, researchers carried out linkage analysis, a method used to map genes on chromosomes and establish their relative distances.
Results and Conclusion
- The study found significant linkage for 124 genetic locations (loci), and of these, 95 were clearly ordered through multipoint analysis, which helps in determining the most likely order of genes.
- The average gap between the markers on the genetic map was 14.2 centimorgan (cM), a unit of measure of genetic linkage.
- The markers were distributed across 29 linkage groups, representing semi-independent chromosome segments.
- A more thorough analysis including synteny group data (genes located on the same chromosome) and Fluorescent In-Situ Hybridization (FISH) data suggested coverage of 26 out of the 31 horse autosomes, which are non-sex chromosomes.
- The comprehensive map created through this research spans a total of 936 cM.
Cite This Article
APA
Guérin G, Bailey E, Bernoco D, Anderson I, Antczak DF, Bell K, Binns MM, Bowling AT, Brandon R, Cholewinski G, Cothran EG, Ellegren H, Förster M, Godard S, Horin P, Ketchum M, Lindgren G, McPartlan H, Mériaux JC, Mickelson JR, Millon LV, Murray J, Neau A, Røed K, Ziegle J.
(1999).
Report of the International Equine Gene Mapping Workshop: male linkage map.
Anim Genet, 30(5), 341-354.
https://doi.org/10.1046/j.1365-2052.1999.00510.x Publication
Researcher Affiliations
- INRA Centre de Recherches de Jouy, Jouy-en-Josas, France.
MeSH Terms
- Animals
- Education
- Genetic Markers
- Genotype
- Horses / genetics
- Male
- Microsatellite Repeats
- Physical Chromosome Mapping
Citations
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