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Home / Equine Research Bank / Vaccine / Response of ELA-A1 horses immunized with lipopeptide contain...
Vaccine2003; 21(5-6); 491-506; doi: 10.1016/s0264-410x(02)00474-7

Response of ELA-A1 horses immunized with lipopeptide containing an equine infectious anemia virus ELA-A1-restricted CTL epitope to virus challenge.

Abstract: Lipopeptide containing an ELA-A1-restricted cytotoxic T lymphocyte (CTL) epitope from the envelope surface unit (SU) protein of the EIAV(WSU5) strain was used to immunize three horses having the ELA-A1 haplotype. Peptide-specific ELA-A1-restricted CTL were induced in all three horses, although these were present transiently in PBMC. These horses were further immunized with lipopeptide containing the corresponding CTL epitope from the EIAV(PV) strain. Then, the three immunized horses and three non-immunized horses were challenged by intravenous inoculation with 300 TCID(50) EIAV(PV). All horses developed cell free viremia, fever and thrombocytopenia. However, there was a statistically lower fever and thrombocytopenia severity score in the immunized group. Shorter duration of plasma viral load in two of the three immunized horses likely explains the less severe clinical disease in this group. Results indicate that lipopeptide immunization had a protective effect against development of clinical disease following virus challenge.
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Publication Date: 2003-01-18 PubMed ID: 12531649DOI: 10.1016/s0264-410x(02)00474-7Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examined the impact of lipopeptide immunization in ELA-A1 horses challenged with the equine infectious anemia virus, showing the immunization had a protective effect against the development of clinical disease.

Study Design and Methodology

  • The researchers used a lipopeptide containing an ELA-A1-restricted cytotoxic T lymphocyte (CTL) epitope, derived from the surface unit (SU) protein of the EIAV(WSU5) strain of the equine infectious anemia virus.
  • Three horses possessing the ELA-A1 haplotype were immunized with this lipopeptide.
  • After the initial immunization, these horses were further immunized with a lipopeptide containing a similar CTL epitope from the EIAV(PV) strain.
  • The study then involved the intravenous inoculation of the three immunized horses, alongside three non-immunized horses, with EIAV(PV).

Study Findings

  • All horses developed symptoms of the virus, including cell-free viremia, fever, and thrombocytopenia.
  • However, the immunized group displayed statistically lower severity scores for fever and thrombocytopenia compared to the non-immunized group.
  • The researchers observed a shorter duration in plasma viral load in two out of the three immunized horses, which they hypothesize as the reason for less severe clinical disease in this group.
  • The researchers concluded that lipopeptide immunization had a protective effect against the development of clinical disease following virus challenge.

Significance of the Study

  • The research underscores the potential of lipopeptide immunization to protect against the development of the equine infectious ania virus.
  • This insight could guide future studies and treatments for the equine infectious anemia virus by using immunization as a preventive measure.

Cite This Article

APA
Ridgely SL, Zhang B, McGuire TC. (2003). Response of ELA-A1 horses immunized with lipopeptide containing an equine infectious anemia virus ELA-A1-restricted CTL epitope to virus challenge. Vaccine, 21(5-6), 491-506. https://doi.org/10.1016/s0264-410x(02)00474-7

Publication

Vaccine
ISSN: 0264-410X
NlmUniqueID: 8406899
Country: Netherlands
Language: English
Volume: 21
Issue: 5-6
Pages: 491-506

Researcher Affiliations

Ridgely, Sherritta L
  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA.
Zhang, Baoshan
    McGuire, Travis C

      MeSH Terms

      • Amino Acid Substitution
      • Animals
      • Cells, Cultured
      • Epitopes / immunology
      • Equine Infectious Anemia / blood
      • Equine Infectious Anemia / immunology
      • Equine Infectious Anemia / prevention & control
      • Haplotypes
      • Horses / immunology
      • Immunization
      • Infectious Anemia Virus, Equine / immunology
      • Lipoproteins / immunology
      • Platelet Count
      • RNA, Viral / analysis
      • RNA, Viral / biosynthesis
      • T-Lymphocytes, Cytotoxic / immunology
      • Thrombocytopenia / blood
      • Thrombocytopenia / immunology

      Grant Funding

      • AI01548 / NIAID NIH HHS
      • AI07025 / NIAID NIH HHS
      • AI24291 / NIAID NIH HHS

      Citations

      This article has been cited 6 times.
      1. Basto AP, Leitão A. Targeting TLR2 for vaccine development.. J Immunol Res 2014;2014:619410.
        doi: 10.1155/2014/619410pubmed: 25057505google scholar: lookup
      2. Baszler TV, Shkap V, Mwangi W, Davies CJ, Mathison BA, Mazuz M, Resnikov D, Fish L, Leibovitch B, Staska LM, Savitsky I. Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites.. Clin Vaccine Immunol 2008 Apr;15(4):659-67.
        doi: 10.1128/CVI.00436-07pubmed: 18305105google scholar: lookup
      3. Tagmyer TL, Craigo JK, Cook SJ, Even DL, Issel CJ, Montelaro RC. Envelope determinants of equine infectious anemia virus vaccine protection and the effects of sequence variation on immune recognition.. J Virol 2008 Apr;82(8):4052-63.
        doi: 10.1128/JVI.02028-07pubmed: 18234792google scholar: lookup
      4. Mealey RH, Lee JH, Leib SR, Littke MH, McGuire TC. A single amino acid difference within the alpha-2 domain of two naturally occurring equine MHC class I molecules alters the recognition of Gag and Rev epitopes by equine infectious anemia virus-specific CTL.. J Immunol 2006 Nov 15;177(10):7377-90.
        doi: 10.4049/jimmunol.177.10.7377pubmed: 17082657google scholar: lookup
      5. Fraser DG, Leib SR, Zhang BS, Mealey RH, Brown WC, McGuire TC. Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge.. Clin Diagn Lab Immunol 2005 Aug;12(8):983-93.
        doi: 10.1128/CDLI.12.8.983-993.2005pubmed: 16085917google scholar: lookup
      6. Fraser DG, Mealey RH, McGuire TC. Selecting peptides to optimize Th1 responses to an equine lentivirus using HLA-DR binding motifs and defined HIV-1 Th peptides.. Immunogenetics 2003 Oct;55(7):508-14.
        doi: 10.1007/s00251-003-0600-ypubmed: 12942208google scholar: lookup
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