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Journal of comparative pathology2019; 174; 1-7; doi: 10.1016/j.jcpa.2019.10.002

Retrospective Analysis of Aetiological Agents Associated with Pulmonary Mycosis Secondary to Enteric Salmonellosis in Six Horses by Panfungal Polymerase Chain Reaction.

Abstract: Pulmonary mycosis secondary to enterocolitis is an uncommon diagnosis in equine medicine, but is thought to result from mucosal compromise and translocation of enteric fungi. The aetiological agent associated with translocation is often identified based on fungal culture or hyphal features in histological sections. In order to understand better the aetiological agents involved, six horses diagnosed with Salmonella enteritis and concurrent pulmonary mycosis were identified retrospectively through a database search of veterinary teaching hospital records. Samples from these cases were subjected to polymerase chain reaction and sequencing of the internal transcribed spacer 2 (ITS-2) located between the 5.8S and 28S rRNA genes to identify the aetiological agent involved. Sequencing identified Aspergillus fumigatus, Aspergillus flavus, Fusarium spp., Cladosporium spp. and Curvularia spp. A single case had a dual infection with Fusarium spp. and A. fumigatus.
Copyright © 2019 Elsevier Ltd. All rights reserved.
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Publication Date: 2019-11-26 PubMed ID: 31955794PubMed Central: PMC8212182DOI: 10.1016/j.jcpa.2019.10.002Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the causes behind pulmonary mycosis, a rare fungal disease in horses that often follows gut infections by Salmonella bacteria. The study made use of modern genetic testing techniques and found that a variety of different fungal species were responsible for the disease in a sample group of six horses.

Background of the Study

  • The study acknowledges pulmonary mycosis – a fungal infection of the lungs – to be a rare occurrence in horses. However, when it does occur, it is usually secondary to an infection by Salmonella bacteria.
  • The belief is that this cascade effect results from the initial bacterial attack compromising the gut lining, allowing fungal cells from the gut microbiota to cross into the bloodstream and migrate to the lungs.
  • The primary aim of this research was to identify the species of fungi responsible for causing pulmonary mycosis in horses. It used samples from six horses previously diagnosed with both Salmonella enteritis (a gut infection) and pulmonary mycosis.

Methodology

  • The study relied on a veterinary hospital database to identify relevant cases retrospectively.
  • In order to find out the causative fungal species, the research team used genetic testing methods. They extracted DNA from the samples and amplified a specific region (the ITS-2 region) located between two other significant sequences (the 5.8S and 28S rRNA genes) for further analysis.
  • The amplified ITS-2 regions were then sequenced, allowing the researchers to match them with known fungal sequences and thus identify the species.

Results and Findings

  • The sequencing results revealed that different horses had mycosis caused by different fungal species. These included Aspergillus fumigatus, Aspergillus flavus, Fusarium spp., Cladosporium spp. and Curvularia spp.
  • In one particular case, two different fungal species — Fusarium spp. and A. fumigatus — were found, indicating a dual infection.
  • The variety of fungal species identified in this study suggest a diverse range of fungal species within the gut microbiota of horses that may translocate and cause disease following an initial Salmonella infection.

Cite This Article

APA
Hensel M, Meason-Smith C, Plumlee QD, Myers AN, Coleman MC, Lawhon S, Rodrigues Hoffmann A, Rech RR. (2019). Retrospective Analysis of Aetiological Agents Associated with Pulmonary Mycosis Secondary to Enteric Salmonellosis in Six Horses by Panfungal Polymerase Chain Reaction. J Comp Pathol, 174, 1-7. https://doi.org/10.1016/j.jcpa.2019.10.002

Publication

Journal of comparative pathology
ISSN: 1532-3129
NlmUniqueID: 0102444
Country: England
Language: English
Volume: 174
Pages: 1-7

Researcher Affiliations

Hensel, M
  • Texas A&M University, College Station, Texas, USA.
Meason-Smith, C
  • Texas A&M University, College Station, Texas, USA.
Plumlee, Q D
  • Texas A&M University, College Station, Texas, USA.
Myers, A N
  • Texas A&M University, College Station, Texas, USA.
Coleman, M C
  • Texas A&M University, College Station, Texas, USA.
Lawhon, S
  • Texas A&M University, College Station, Texas, USA.
Rodrigues Hoffmann, A
  • Texas A&M University, College Station, Texas, USA.
Rech, R R
  • Texas A&M University, College Station, Texas, USA. Electronic address: rrech@cvm.tamu.edu.

MeSH Terms

  • Animals
  • Enterocolitis / complications
  • Enterocolitis / veterinary
  • Horse Diseases / microbiology
  • Horses
  • Mycoses / microbiology
  • Mycoses / veterinary
  • Polymerase Chain Reaction
  • Respiratory Tract Infections / microbiology
  • Respiratory Tract Infections / veterinary
  • Retrospective Studies
  • Salmonella Infections, Animal / complications

Grant Funding

  • T32 OD011083 / NIH HHS

Conflict of Interest Statement

Conflict of Interest. The authors declare no conflicts of interest with respect to the publication of this manuscript.

References

This article includes 23 references
  1. Berenguer J, Allende MC, Lee JW, Garrett K, Lyman C, Ali NM, Bacher J, Pizzo PA, Walsh TJ. Pathogenesis of pulmonary aspergillosis. Granulocytopenia versus cyclosporine and methylprednisolone-induced immunosuppression.. Am J Respir Crit Care Med 152, 1079–1086.
    pubmed: 7663787
  2. Blomme EDP, La Perle KMD, Wilkins PA. Aspergillosis in horses: a review.. Equine Veterinary Education 10, 86–93.
  3. Brenchley JM, Douek DC. Microbial translocation across the GI tract.. Annu Rev Immunol 30, 149–173.
    pmc: PMC3513328pubmed: 22224779
  4. Breshears MA, Holbrook TC, Haak CE, York PA. Pulmonary aspergillosis and ischemic distal limb necrosis associated with enteric salmonellosis in a foal.. Vet Pathol 44, 215–217.
    pubmed: 17317800
  5. Carrasco L, Mendez A, Jensen HE. Chronic bronchopulmonary aspergillosis in a horse with Cushing’s syndrome.. Mycoses 39, 443–447.
    pubmed: 9145001
  6. Costa LR, Johnson JR, Baur ME, Beadle RE. Temporal clinical exacerbation of summer pasture-associated recurrent airway obstruction and relationship with climate and aeroallergens in horses.. Am J Vet Res 67, 1635–1642.
    pubmed: 16948614
  7. Fraser RS. Pulmonary aspergillosis: pathologic and pathogenetic features.. Pathol Annu 28 Pt 1, 231–277.
    pubmed: 8416139
  8. Hattel AL, Drake TR, Anderholm BJ, McAllister ES. Pulmonary aspergillosis associated with acute enteritis in a horse.. J Am Vet Med Assoc 199, 589–590.
    pubmed: 1787116
  9. Kaufman L. Laboratory methods for the diagnosis and confirmation of systemic mycosis.. Clin Infect Dis 14, S23–S29.
    pubmed: 1562691
  10. Koh AY, Kohler JR, Coggshall KT, Van Rooijen N, Pier GB. Mucosal damage and neutropenia are required for Candida albicans dissemination.. PLoS Pathog 4, e35.
    pmc: PMC2242836pubmed: 18282097
  11. Kurowski PB, Traub-Dargatz JL, Morley PS, Gentry-Weeks CR. Detection of Salmonella spp in fecal specimens by use of real-time polymerase chain reaction assay.. Am J Vet Res 63, 1265–1268.
    pubmed: 12224858
  12. Manship AJ, Blikslager AT, Elfenbein JR. Disease features of equine coronavirus and enteric salmonellosis are similar in horses.. J Vet Intern Med 33, 912–917.
    pmc: PMC6430874pubmed: 30632200
  13. Marchetti G, Tincati C, Silvestri G. Microbial Translocation in the Pathogenesis of HIV Infection and AIDS.. Clinical Microbiology Reviews 26, 2–18.
    pmc: PMC3553668pubmed: 23297256
  14. Meason-Smith C, Edwards EE, Older CE, Branco M, Bryan LK, Lawhon SD, Suchodolski JS, Gomez G, Mansell J, Hoffmann AR. Panfungal Polymerase Chain Reaction for Identification of Fungal Pathogens in Formalin-Fixed Animal Tissues.. Vet Pathol 54, 640–648.
    pmc: PMC6361388pubmed: 28346123
  15. Nucci M, Anaissie E. Fusarium infections in immunocompromised patients.. Clin Microbiol Rev 20, 695–704.
    pmc: PMC2176050pubmed: 17934079
  16. Pace LW, Wirth NR, Foss RR, Fales WH. Endocarditis and pulmonary aspergillosis in a horse.. J Vet Diagn Invest 6, 504–506.
    pubmed: 7858038
  17. Perusquia-Ortiz AM, Vazquez-Gonzalez D, Bonifaz A. Opportunistic filamentous mycoses: aspergillosis, mucormycosis, phaeohyphomycosis and hyalohyphomycosis.. J Dtsch Dermatol Ges 10, 611–621; quiz 621–612.
    pubmed: 22925358
  18. Rosenstein D, Mullaney T. Acute pulmonary aspergillosis and severe enterocolitis in a quarter horse mare.. Equine Veterinary Education 8, 200–203.
  19. Slocombe RF, Slauson DO. Invasive pulmonary aspergillosis of horses: an association with acute enteritis.. Vet Pathol 25, 277–281.
    pubmed: 3407097
  20. Stergiopoulou T, Meletiadis J, Roilides E, Kleiner DE, Schaufele R, Roden M, Harrington S, Dad L, Segal B, Walsh TJ. Host-dependent patterns of tissue injury in invasive pulmonary aspergillosis.. Am J Clin Pathol 127, 349–355.
    pubmed: 17276936
  21. Sweeney CR, Habecker PL. Pulmonary aspergillosis in horses: 29 cases (1974–1997).. J Am Vet Med Assoc 214, 808–811.
    pubmed: 10101412
  22. Uzal FA, Plattner BL and Hotstetter JM (2007). Alimentary System. Elsevier Saunders, Edinburgh ; New York.
  23. White T, Bruns T, Lee S and Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, Innis M, Gelfand D, Shinsky J and White T, Eds, Academic Press, pp. 315–322.

Citations

This article has been cited 3 times.
  1. Hensel ME, Rodrigues-Hoffmann A, Dray BK, Wilkerson GK, Baze WB, Sulkosky S, Hodo CL. Gastrointestinal tract pathology of the owl monkey (Aotus spp.). Vet Pathol 2024 Mar;61(2):316-323.
    doi: 10.1177/03009858231204260pubmed: 37830482google scholar: lookup
  2. Amory H, Cesarini C, De Maré L, Loublier C, Moula N, Detilleux J, Saulmont M, Garigliany MM, Lecoq L. Relationship between the Cycle Threshold Value (Ct) of a Salmonella spp. qPCR Performed on Feces and Clinical Signs and Outcome in Horses. Microorganisms 2023 Jul 30;11(8).
    doi: 10.3390/microorganisms11081950pubmed: 37630510google scholar: lookup
  3. Rahman A, Uzal FA, Hassebroek AM, Carvallo FR. Retrospective study of pneumonia in non-racing horses in California. J Vet Diagn Invest 2022 Jul;34(4):587-593.
    doi: 10.1177/10406387221094273pubmed: 35535386google scholar: lookup
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