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Home / Equine Research Bank / Infect Immun / Rhodococcus equi-specific cytotoxic T lymphocytes in immune ...
Infection and immunity2005; 73(4); 2083-2093; doi: 10.1128/IAI.73.4.2083-2093.2005

Rhodococcus equi-specific cytotoxic T lymphocytes in immune horses and development in asymptomatic foals.

Abstract: Rhodococcus equi is an important cause of pneumonia in young horses; however, adult horses are immune due to their ability to mount protective recall responses. In this study, the hypothesis that R. equi-specific cytotoxic T lymphocytes (CTL) are present in the lung of immune horses was tested. Bronchoalveolar lavage (BAL)-derived pulmonary T lymphocytes stimulated with R. equi lysed infected alveolar macrophages and peripheral blood adherent cells (PBAC). As with CTL obtained from the blood, killing of R. equi-infected targets by pulmonary effectors was not restricted by equine lymphocyte alloantigen-A (ELA-A; classical major histocompatibility complex class I), suggesting a novel or nonclassical method of antigen presentation. To determine whether or not CTL activity coincided with the age-associated susceptibility to rhodococcal pneumonia, CTL were evaluated in foals. R. equi-stimulated peripheral blood mononuclear cells (PBMC) from 3-week-old foals were unable to lyse either autologous perinatal or mismatched adult PBAC targets. The defect was not with the perinatal targets, as adult CTL effectors efficiently killed infected targets from 3-week-old foals. In contrast, significant CTL activity was present in three of five foals at 6 weeks of age, and significant specific lysis was induced by PBMC from all foals at 8 weeks of age. As with adults, lysis was ELA-A unrestricted. Two previously described monoclonal antibodies, BCD1b3 and CD1F2/1B12.1, were used to examine the expression of CD1, a nonclassical antigen-presenting molecule, on CTL targets. These antibodies cross-reacted with both foal and adult PBAC. However, neither antibody bound alveolar macrophages, suggesting that the R. equi-specific, major histocompatibility complex-unrestricted lysis is not restricted by a surface molecule identified by these antibodies.
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Publication Date: 2005-03-24 PubMed ID: 15784549PubMed Central: PMC1087435DOI: 10.1128/IAI.73.4.2083-2093.2005Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the presence of Rhodococcus equi-specific cytotoxic T lymphocytes in adult horses’ lungs and explores its development in young horses (foals) in regards to their vulnerability to pneumonia caused by R. equi.

Understanding Rhodococcus equi and Immune Responses in Horses

  • Rhodococcus equi is a significant cause of pneumonia in foals. Nevertheless, adult horses are immune due to their capability to generate protective responses against R. equi.
  • The research puts forward the hypothesis that R. equi-specific cytotoxic T lymphocytes (CTLs) are present in the lung of immune horses. These T lymphocytes were stimulated using R. equi, which destroyed infected alveolar macrophages and peripheral blood adherent cells (PBACs).

Nonclassic Method of Antigen Presentation

  • The killing of R. equi-infected targets by these T cells does not abide by the equine lymphocyte alloantigen-A (ELA-A), an element of the classical major histocompatibility complex class I. This suggests a nontraditional or new method of antigen presentation.

Research into Pneumonia Susceptibility in Foals

  • To explore whether CTL activity aligns with the age-related susceptibility to the pneumonia caused by R. equi, the researchers investigated CTL in foals.
  • The research found that peripheral blood mononuclear cells (PBMCs) from 3-week-old foals stimulated with R. equi were impotent to destruct either self-originating perinatal or mismatched adult PBAC targets.

Subsequent CTL Activity in Foals

  • A significant CTL activity was recorded in three out of five foals at 6 weeks of age.
  • By 8 weeks of age, significant lysis was induced by PBMCs from all foals with ELA- A unrestricted lysis, similar to adult horses.

Expression of CD1 on CTL targets

  • Two antibodies, BCD1b3 and CD1F2/1B12.1, were used to assess CD1 expression, a non-classical antigen-presenting molecule, on CTL targets.
  • Though both antibodies bound to PBACs from foals and adults, they did not bind to alveolar macrophages, implying R. equi-specific lysis is not confined to a surface molecule recognized by these antibodies.

Cite This Article

APA
Patton KM, McGuire TC, Hines MT, Mealey RH, Hines SA. (2005). Rhodococcus equi-specific cytotoxic T lymphocytes in immune horses and development in asymptomatic foals. Infect Immun, 73(4), 2083-2093. https://doi.org/10.1128/IAI.73.4.2083-2093.2005

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 73
Issue: 4
Pages: 2083-2093

Researcher Affiliations

Patton, Kristin M
  • Department of Veterinary Microbiology and Pathology, Washington State University, Bustad Hall, Room 402, Pullman, Washington 99164-7040, USA.
McGuire, Travis C
    Hines, Melissa T
      Mealey, Robert H
        Hines, Stephen A

          MeSH Terms

          • Actinomycetales Infections / immunology
          • Actinomycetales Infections / veterinary
          • Animals
          • Antigens, CD1 / analysis
          • Horse Diseases / immunology
          • Horses
          • Lung / immunology
          • Pneumonia, Bacterial / immunology
          • Pneumonia, Bacterial / veterinary
          • Rhodococcus equi / immunology
          • T-Lymphocytes, Cytotoxic / immunology

          Grant Funding

          • 5 K08 A1049391-03 / PHS HHS

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          This article has been cited 13 times.
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