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Acta tropica2022; 233; 106543; doi: 10.1016/j.actatropica.2022.106543

Risk factors for equine trypanosomosis and hematological analysis of horses in Paraguay.

Abstract: Animal trypanosomosis, caused by Trypanozoon trypanosomes (Trypanosoma evansi and T. equiperdum), and Trypanosoma vivax, is endemic to South American countries and has a negative impact on the livestock industry. However, the risk factors for trypanosomosis in Paraguay remain unknown. This study aimed to determine the risk factors for equine trypanosomosis in Paraguay based on a PCR-based molecular survey and individual horse sampling data. In this study, 739 blood samples were collected from horses in 16 departments of Paraguay between August 2019 and November 2020. To elucidate the risk factors for trypanosome infection, the relationship between trypanosome infection status detected by PCR and the location, sex, age, breed of horses, and season of sample collection was analyzed. There were no significant differences in trypanosome prevalence in horses between the eastern and western regions, ages, or breeds of horses in Paraguay. Sex and season were identified as risk factors for trypanosome infection in horses in Paraguay in the current study. These results suggest that the rainy-summer season, when vectors increase in number and their blood-sucking activity, could be the most important risk factor for trypanosome infection in Paraguay horses. Preventive measures and treatments should be developed to address these factors.
Publication Date: 2022-05-25 PubMed ID: 35643185DOI: 10.1016/j.actatropica.2022.106543Google Scholar: Lookup
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  • Journal Article

Summary

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This research study aimed to identify the risk factors for equine trypanosomosis, a disease affecting horses in Paraguay. Findings indicated that while location, age, and breed of horses did not significantly affect infection rates, sex and seasonality did, with the infection rates being higher during the rainy-summer season.

Introduction

  • The research is focused on Trypanosoma, a breed of parasite causing the disease Trypanosomosis in horses.
  • Equine trypanosomosis has a significant negative impact on the South America’s livestock industry, and as of this study’s filming, the risk factors for this disease in Paraguay were unknown.
  • This research sought to identify the possible risk factors, harnessing data based on molecular survey results from PCR testing and individual horse sampling.

Methodology

  • 739 blood samples were collected from horses in 16 different regions of Paraguay over a period between August 2019 and November 2020.
  • The collected samples were analyzed using Polymerase Chain Reaction (PCR) technology to identify Trypanosome infection.
  • The researchers then evaluated the possible relationship between Trypanosome infection and different variables, namely, the location, sex, age, breed of horses, and the season of sample collection.

Results

  • Proof of Trypanosome infection was not found significant differences across the various horse breeds, their ages, or regions of Paraguay.
  • However, the research study did find horse’s sex and the season of sample collection as significant risk factors for Trypanosome infection in Paraguay.
  • Specifically, the rainy-summer season was identified as the most risky period for Trypanosome infection among horses in Paraguay.
  • This conclusion has been drawn upon the fact that the number and activity of vectors (an organism, typically a biting insect or tick, that transmits a disease or parasite from one animal or plant to another) increase during the rainy-summer season, thus increasing the chances of Trypanosome infection.

Implications

  • Understanding these risk factors could help inform programs aimed at controlling or eliminating this disease.
  • This study’s conclusion suggests that efforts to develop preventive measures or treatment should focus on protecting horses during the rainy-summer season and consider the different risks for male and female horses.

Cite This Article

APA
Yamazaki A, Suganuma K, Kayano M, Acosta TJ, Saitoh T, Valinotti MFR, Sanchez AR, Inoue N. (2022). Risk factors for equine trypanosomosis and hematological analysis of horses in Paraguay. Acta Trop, 233, 106543. https://doi.org/10.1016/j.actatropica.2022.106543

Publication

ISSN: 1873-6254
NlmUniqueID: 0370374
Country: Netherlands
Language: English
Volume: 233
Pages: 106543
PII: S0001-706X(22)00235-2

Researcher Affiliations

Yamazaki, Ai
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
Suganuma, Keisuke
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan; Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: k.suganuma@obihiro.ac.jp.
Kayano, Mitsunori
  • Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: kayano@obihiro.ac.jp.
Acosta, Tomás J
  • Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: tjacosta@obihiro.ac.jp.
Saitoh, Tomoko
  • Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: tsaitoh@obihiro.ac.jp.
Valinotti, Maria Fátima Rodríguez
  • Centro de Diagnostico Veterinario, San Lorenzo, Paraguay. Electronic address: m.rodriguez@cedivep.com.py.
Sanchez, Antonio Rodríguez
  • Centro de Diagnostico Veterinario, San Lorenzo, Paraguay. Electronic address: arodriguez@cedivep.com.py.
Inoue, Noboru
  • National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan. Electronic address: ircpmi@obihiro.ac.jp.

MeSH Terms

  • Animals
  • Blood / parasitology
  • Female
  • Horse Diseases / diagnosis
  • Horse Diseases / epidemiology
  • Horses
  • Male
  • Paraguay / epidemiology
  • Polymerase Chain Reaction / veterinary
  • Prevalence
  • Risk Factors
  • Trypanosoma / genetics
  • Trypanosoma / isolation & purification
  • Trypanosomiasis / diagnosis
  • Trypanosomiasis / epidemiology
  • Trypanosomiasis / veterinary

Citations

This article has been cited 4 times.
  1. Ramos CJR, de Souza Franco C, da Luz SP, Marques J, de Souza KM, do Nascimento LFN, das Neves GB, Moreira RS, Miletti LC. First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America. Parasit Vectors 2023 Jan 5;16(1):4.
    doi: 10.1186/s13071-022-05562-7pubmed: 36604766google scholar: lookup
  2. Raftery AG, Gummery L, Garcia K, Mohite D, Capewell P, Sutton DGM. Equine trypanosomiasis, a systematic review and meta-analyses: Prevalence, morbidity and mortality. Equine Vet J 2026 Mar;58(2):291-319.
    doi: 10.1111/evj.70101pubmed: 41131780google scholar: lookup
  3. Hacilarlioglu S, Bilgic HB, Karagenc T, Aydin HB, Toker H, Kanlioglu H, Pekagirbas M, Bakirci S. Molecular Detection and Prevalence of Equine Piroplasmosis and Other Blood Parasites in Equids of Western Aegean Türkiye. Vet Sci 2025 Aug 27;12(9).
    doi: 10.3390/vetsci12090826pubmed: 41012752google scholar: lookup
  4. Hébert L, Froger D, Madeline A, Lecouturier F, Lemans C, Zientara S. European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test. Vet Sci 2023 Sep 26;10(10).
    doi: 10.3390/vetsci10100592pubmed: 37888544google scholar: lookup