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Journal of clinical microbiology1992; 30(1); 59-62; doi: 10.1128/jcm.30.1.59-62.1992

Rotavirus serotype G3 predominates in horses.

Abstract: Foal fecal group A rotavirus strains were characterized by electropherotype, serotype, and subgroup and shown to be distinctly different from rotaviruses of other mammals. Of 86 strains that were electropherotyped, 98% had similar profiles, with gene segments 3 and 4 close together and segments 7, 8, and 9 widely spaced. Of 70 strains that had sufficient detectable VP7 antigen to be serotyped by enzyme-linked immunosorbent assays (ELISAs), 63% were serotype G3 (39% were subtype G3A and 24% were subtype G3B), 4% were serotype G13, and 33% were untypeable. Serotypes G1, G2, G4, G5, G6, G9, G10, and G14 were not detected, although G5 and G14 strains have been identified among cultivable equine strains. Of 50 strains that had sufficient detectable VP6 antigen to be subgrouped by ELISAs, only 12% were able to be assigned to either subgroup I or II, with the remaining 88% belonging to neither subgroup.
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Publication Date: 1992-01-01 PubMed ID: 1310333PubMed Central: PMC264996DOI: 10.1128/jcm.30.1.59-62.1992Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research finds that serotype G3 is most prevalent in equine rotavirus strains commonly found in foal feces. Although other serotypes and subgroups exist, G3, specifically subtypes G3A and G3B, dramatically dominate in horse populations.

Overview of Research Methods

The researchers collected and examined foal fecal rotavirus strains. By using electropherotype, serotype, and subgroup characterization methods, they were able to identify the specific types of rotaviruses found in horses. The report outlines the results from characterizing 86 electropherotyped strains and 70 serotyped strains, which were analyzed for VP7 antigen presence, and 50 strains tested for VP6 antigen to be subgrouped.

  • Electropherotyping: This method differentiates virus strains based on patterns of RNA migration in an electric field.
  • Serotyping and Subgrouping: These techniques identify the serological differences among viral strains. In this case, researchers sought the presence of VP7 and VP6 antigens.

Key Findings from the Research

The studies found that rotavirus strains from horse populations are distinct from those discovered in other mammals.

  • 98% of the electropherotyped strains shared similar profiles, which identifies them as a specific and distinctive rotavirus strain in the horse population.
  • Within the serotyped strains, 63% were identified as serotype G3. More specifically, 39% belonged to subtype G3A and 24% to subtype G3B.
  • Other serotypes such as G1, G2, G4, G5, G6, G9, G10, and G14 were not detected in the samples, although G5 and G14 have previously been found among cultivable equine strains.
  • In subgrouping based on presence of VP6 antigen, only 12% of the strains could be assigned to either subgroup I or II, and 88% did not belong to either of those subgroups.

Implications of the Research

Understanding the dominant rotavirus strains in horses is critical for effective disease management and control. It is fundamental for the development of accurate diagnostic tools and effective vaccines. Moreover, this research also provides insights into the specific characteristics of equine rotaviruses, which could be beneficial in further studies mapping rotavirus evolution and cross-species transmission.

Cite This Article

APA
Browning GF, Chalmers RM, Fitzgerald TA, Corley KT, Campbell I, Snodgrass DR. (1992). Rotavirus serotype G3 predominates in horses. J Clin Microbiol, 30(1), 59-62. https://doi.org/10.1128/jcm.30.1.59-62.1992

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 30
Issue: 1
Pages: 59-62

Researcher Affiliations

Browning, G F
  • Mordun Research Institute, Edinburgh, Scotland.
Chalmers, R M
    Fitzgerald, T A
      Corley, K T
        Campbell, I
          Snodgrass, D R

            MeSH Terms

            • Animals
            • Diarrhea / microbiology
            • Diarrhea / veterinary
            • Enzyme-Linked Immunosorbent Assay
            • Feces / microbiology
            • Horse Diseases / microbiology
            • Horses
            • Rotavirus / classification
            • Rotavirus / immunology
            • Rotavirus / isolation & purification
            • Rotavirus Infections / microbiology
            • Rotavirus Infections / veterinary
            • Serotyping

            Grant Funding

            • Wellcome Trust

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            Citations

            This article has been cited 18 times.
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