Scanning electron microscopy and microbiological evaluation of equine burn wound repair after platelet-rich plasma gel treatment.
Abstract: Deep second-degree burns (DSDb) involve all epidermis layers, including the basal laminae. In general, DSDb heals with extensive areas of scarring. Platelet-rich plasma (PRP) is a source of growth factors, obtained by centrifugation and addition of thrombin and calcium, resulting in a gel. The objective of this study was to monitor the healing of DSDb by sequential biopsies after PRP treatment and evaluating by a scanning electron microscopy. Also, characterize bacterial profile and PRP antibacterial effect in horses' burns. Four horses were placed into two groups, G1 and G2, with the groups receiving 1 and 2 treatments of PRP, respectively. Control groups (Gc1 and Gc2) were treated with saline solution. DSDb were made by infliction of hot iron after epidermis dissection. Biopsies were performed 5, 15, 25 and 40 days after PRP treatment, and swab collections were performed 30 days after PRP treatment. At d25, G1 showed a higher amount of fibrils compared with Gc1, and the organization of G2 was similar to that of uninjured tissue. At d40, G1 resembled intact tissue and G2 showed dense tissue. All groups had bacterial contamination but no infection. PRP accelerated repair, induced fibroses and probably provided antibacterial activity in horses with DSDb.
Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.
Publication Date: 2012-06-07 PubMed ID: 22683140DOI: 10.1016/j.burns.2012.02.029Google Scholar: Lookup
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- Journal Article
Summary
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The research article investigates the effect of platelet-rich plasma (PRP) gel treatment on the healing of deep second-degree burns in horses, and its potential antibacterial properties. The study used scanning electron microscopy to monitor the healing process and investigated the bacterial profile of the burns.
Objective
The main aim of the research was to examine the healing progress and bacterial profile of deep second-degree burns (DSDb) in horses, after they were treated with platelet-rich plasma (PRP) gel. PRP gel is known to be a source of growth factors beneficial for healing and was applied to the studied burn wounds to track its impact.
Methodology
- The researchers used four horses as their test subjects which were divided into two main groups, G1 and G2, and two control groups, Gc1 and Gc2. Each of the main groups received either one or two treatments of PRP gel respectively, while the control groups were treated with a saline solution.
- The deep second-degree burns were inflicted on the horses by applying a hot iron after dissecting the epidermis.
- The researchers monitored the healing process by taking biopsies after 5, 15, 25, and 40 days post-PRP treatment. In addition, swab collections were performed 30 days after PRP treatment to assess bacterial colonization.
Results
- At day 25 post-treatment, group G1 showed more fibrils compared to its control group, Gc1, while the tissue of G2 resembled that of uninjured tissue.
- On day 40, G1’s burn tissue was similar to intact skin and G2 showcased dense tissue fibrosis.
- All groups experienced bacterial contamination, but this did not result in any infection, suggesting that the PRP treatment may also have antibacterial properties.
Conclusion
The study concluded that PRP treatment facilitated expedited healing and the formation of new fibrous tissue in the burns, which is a key step in reducing scarring. It also potentially provided antibacterial activity, as all treated burns had bacterial colonization but did not develop infections.
Cite This Article
APA
Maciel FB, DeRossi R, Módolo TJ, Pagliosa RC, Leal CR, Delben AA.
(2012).
Scanning electron microscopy and microbiological evaluation of equine burn wound repair after platelet-rich plasma gel treatment.
Burns, 38(7), 1058-1065.
https://doi.org/10.1016/j.burns.2012.02.029 Publication
Researcher Affiliations
- Department of Veterinary Medicine-Surgery and Anesthesiology, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil.
MeSH Terms
- Animals
- Anti-Bacterial Agents / therapeutic use
- Burns / microbiology
- Burns / pathology
- Burns / therapy
- Enterobacter / isolation & purification
- Gels / therapeutic use
- Horses
- Intercellular Signaling Peptides and Proteins / therapeutic use
- Male
- Microscopy, Electron, Scanning
- Platelet-Rich Plasma
- Serratia / isolation & purification
- Staphylococcus / isolation & purification
- Wound Healing / drug effects
Citations
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- Berni P, Leonardi F, Conti V, Ramoni R, Grolli S, Mattioli G. Case Report: A Novel Ventilated Thermoplastic Mesh Bandage for Post-operative Management of Large Soft Tissue Defects: A Case Series of Three Dogs Treated With Autologous Platelet Concentrates. Front Vet Sci 2021;8:704567.
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