FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Microscopy research and technique2021; 85(5); 1643-1653; doi: 10.1002/jemt.24024

Scanning electron microscopy of the nasal skin in different animal species as a method for forensic identification.

Abstract: The current study aims to provide a new method for the identification of animal species by using the scanning electron microscopic study on the nasal skin around the nostril. The reference databases for animal identification using traditional ways (i.e., hair and bone analyses) were somewhat available. This study was conducted on the nasal skin of seven different animal species: large ruminants (Saidi buffalo and frozen Angus cattle); small ruminants (Egyptian goat and Rahmani sheep); camels (one-hump dromedary camel); equines (African domestic donkey and Arabian horse); carnivores (farm domestic cat, Egyptian Baladi dog, and red fox); swine (Danish Landrace pig); and rodents (albino rat and New Zealand white rabbit). The nasal skin was divided by grooves into different shaped raised areas in some examined animals, the mean diameter of these areas and width of the grooves of each animal species were recorded. The characteristic differential features of the nasal skin of each animal species have been documented. Few openings of the nasolabial glands were demonstrated in large ruminants and swine, which were H letter-shaped in buffaloes, oval-shaped in cattle, and whirlpool-shaped with several layers of folds on their margin in pig. Moreover, the openings of the lateral nasal glands were whirlpool, ovoid, and oval shaped in goats, sheep, and camels, respectively. In conclusion, the present study proved that the skin around the nostrils plays an important role as means of identification in forensic investigations and improves the field of veterinary forensic medicine in general, which is not well-developed in comparison with human forensics.
© 2021 Wiley Periodicals LLC.
Get Full Text
Publication Date: 2021-12-13 PubMed ID: 34904324DOI: 10.1002/jemt.24024Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on a novel approach to identify animal species by scanning the skin around the nostril using electron microscopy. The study involved the examination of seven different animal species, with the findings suggesting the potential for this method in forensic investigations and veterinary forensics.

Objective of the Study

  • The main objective of this research was to discover a new method for the identification of animal species. The researchers used scanning electron microscopic study on the nasal skin around the nostril, an area that has been less researched for species identification purposes.

Methodology

  • The study was conducted on a variety of animals including large ruminants like Saidi buffalo and Angus cattle, small ruminants like Egyptian goat and Rahmani sheep, a camel, equines like African domestic donkey and Arabian horse, carnivores like farm domestic cat, Egyptian Baladi dog, and red fox, swines, and rodents.
  • The nasal skin of these animals was examined, specifically focusing on the grooves and raised areas. The mean diameter of these raised areas and the width of the grooves for each of the animal species were recorded.

Findings

  • The researchers documented unique features of the nasal skin for each animal species, suggesting that these could be used for species identification. They also observed the openings of the nasolabial glands in large ruminants and swines, which presented unique shapes across different species.
  • For instance, the openings were H letter-shaped in buffaloes, oval-shaped in cattle, and whirlpool-shaped with folded margins in pigs. The openings of the lateral nasal glands in goats, sheep, and camels were also discriminated based on their special shapes.

Conclusion

  • The results from this study indicated that the skin around the nostrils could be a significant identifier for forensic investigations. By applying scanning electron microscopy, the field of veterinary forensic medicine could develop further, given that it has been relatively less explored compared to human forensics.

Cite This Article

APA
Madkour FA, Abdelsabour-Khalaf M. (2021). Scanning electron microscopy of the nasal skin in different animal species as a method for forensic identification. Microsc Res Tech, 85(5), 1643-1653. https://doi.org/10.1002/jemt.24024

Publication

Microscopy research and technique
ISSN: 1097-0029
NlmUniqueID: 9203012
Country: United States
Language: English
Volume: 85
Issue: 5
Pages: 1643-1653

Researcher Affiliations

Madkour, Fatma A
  • Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
Abdelsabour-Khalaf, Mohammed
  • Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.

MeSH Terms

  • Animals
  • Buffaloes
  • Camelus
  • Cats
  • Cattle
  • Dogs
  • Forensic Medicine
  • Goats
  • Horses
  • Microscopy, Electron, Scanning
  • Rabbits
  • Rats
  • Sheep
  • Swine

References

This article includes 33 references
  1. Ahmed Y, Abdelsabour-Khalaf M, Khalil F. Absence of typical Haversian system from the compact bone of some reptile and bird species. Asian Journal of Biological Sciences 10, 98-103.
  2. Ahmed YA, Ali S, Ghallab A. Hair histology as a tool for forensic identification of some domestic animal species. EXCLI Journal 17, 663.
  3. Andrews PM. Microplicae: Characteristic ridge-like folds of the plasmalemma. The Journal of Cell Biology 68(3), 420-429.
  4. Bhat MA, Shrivastav AB, Dar AA, Bari SW. Studies on hair of some wild animals for species identification as an aid to wildlife forensics. International Journal for Agro Veterinary and Medical Sciences 8(1), 2-5.
  5. Bhattacharya M, Sheikh I, Rajkhowa J. Epidermal thickness in the skin of Yak (Poephagus poephagus). Indian Journal of Veterinary Anatomy 15, 73-76.
  6. Budras K-D, Habel RE, Wünsche A, Buda S. Bovine anatomy: An illustrated text (1st ed.). Hannover, Germany: Schlütersche.
  7. Budras K-D, Sack WO, Rock S, Aaron H, Rolf B. Anatomy of the horse: An illustrated text (5th ed.). Hannover, Germany: Schlütersche.
  8. Clifford DH. Preanesthesia, anesthesia, analgesia and euthanasia (p. 556). Orlando, FL: Academic Press.
  9. Coldea N. Nose prints as a method of identification in dogs. Veterinary Quarterly 16(sup1), 60-60.
  10. Cortellini V, Carobbio A, Brescia G, Cerri N, Verzeletti A. A comparative study of human and animal hairs: Microscopic hair comparison and cytochrome c oxidase I species identification. Journal of Forensic Science and Medicine 5(1), 20-23.
  11. Dellmann HD. Textbook of veterinary histology (4th ed.). Philadelphia, PA: Lea and Febiger.
  12. Dyce KM, Sack WO, Wensing CJG. Text book of veterinary anatomy (5th ed.). St. Louis, MO: Saunders/Elsevier.
  13. El-Hagri MAA. Splanchnology of domestic animals. In Respiratory system (1st ed., pp. 1-31). Cairo, Egypt: Cairo University Press.
  14. Horning JG, McKee AJ, Keller HE, Smith KK. Nose printing your cat and dog patients. Veterinary Medicine 21, 432-453.
  15. Karczmarczyk S, Gryzińska M. Identification value of dog (Canis lupus familiaris) and cat (Felis catus) hair based on selected features of morphology in the context of forensic examinations. Journal of Forensic Practice 306(4), 59-70.
  16. Knecht L. The use of hair morphology in the identification of mammals. In J. E. Huffman & J. R. Wallace (Eds.), Wildlife forensics: Methods and applications (Vol. 8, pp. 129-143). Chichester, England: Wiley.
  17. Kshirsagar S, Singh B, Fulari S. Comparative study of human and animal hair in relation with diameter and medullary index. Indian Journal of Forensic Medicine and Pathology 2(3), 105-108.
  18. Lawton MPC, Cooper JE. Wildlife crime scene visits. Applied Herpetology 6, 29-45.
  19. Madkour F, Abdelsabour-Khalaf M. Gross anatomical and morphometrical study of the nasal cavity (cavum nasi) of Egyptian goat (Capra hircus) and Egyptian Baladi dog (Canis lupus). SVU-International Journal of Veterinary Sciences 4(3), 80-93.
  20. Madkour FA, Mohamed AA. Comparative anatomical studies on the glandular stomach of the rock pigeon (Columba livia targia) and the Egyptian laughing dove (Streptopelia senegalensis aegyptiaca). Anatomia, Histologia, Embryologia 48(1), 53-63.
  21. Madkour FA, Mohamed SA, Abdalla KEH, Ahmed YA. Developmental stages and growth of the proventriculus of post-hatching Muscovy duck: Light and electron microscopic study. Microscopy Research and Technique 1-15.
    doi: 10.1002/jemt.23884google scholar: lookup
  22. Madkour FA, Mohammed ES. Histomorphological investigations on the lips of Rahmani sheep (Ovis aries): A scanning electron and light microscopic study. Microscopy Research and Technique 84(5), 992-1002.
  23. Madkour FA, Mohammed ESI, Radey R, Abdelsabour-Khalaf M. Morphometrical, histological, and scanning electron microscopic investigations on the hard palate of Rahmani sheep (Ovis aries). Microscopy Research and Technique 1-14.
    doi: 10.1002/jemt.23887google scholar: lookup
  24. Matar A, Silverman H. Perspectives of Egyptian research ethics committees regarding their effective functioning. Journal of Empirical Research on Human Research Ethics 8(1), 32-44.
  25. Meyer W, Neurand K. A comparative scanning electron microscopic view of the integument of domestic mammals. Scanning Microscopy 1(1), 169-180.
  26. Meyer W, Schwarz R, Neurand K. The skin of domestic mammals as a model for the human skin, with special reference to the domestic pig. Current Problems in Dermatology 7, 39-52.
  27. Nickel R, Shummer A, Seiferle E. The viscera of the domestic animals (2nd ed., pp. 211-281). New York, NY: Springer.
  28. Nishant K, Vrijesh K, Ajay K. Wildlife forensic: Current techniques and their limitations. Forensic Science Criminal Journal 5(4), 402.
  29. Nomina Anatomica Veterinaria. Electronic edition. Published by the international committees on veterinary gross anatomical nomenclature under the financial responsibility of the world association of veterinary anatomists. New York: Zurich and Ithaca.
  30. Petersen W. The identification of the bovine by means of nose-prints. Journal of Dairy Science 5(3), 249-258.
  31. Soliman SA, Madkour FA. Developmental events and cellular changes occurred during esophageal development of quail embryos. Scientific Reports 11(1), 1-21.
  32. Tej P, Pawan K, Gurdial S. Gross anatomy, histology and scanning electron microscopy of the nasal commissures and alae in young pigs. Haryana Veterinarian 55(1), 37-40.
  33. Tridico S. Examination, analysis, and application of hair in forensic science-animal hair. Forensic Science Review 17(1), 17-28.

Citations

This article has been cited 2 times.
  1. Madkour FA, Abdelsabour-Khalaf M. Morphological and ultrastructural features of the laryngeal mound of Egyptian Cattle Egret (Bubulcus ibis, Linnaeus, 1758). BMC Zool 2022 Jul 29;7(1):44.
    doi: 10.1186/s40850-022-00147-4pubmed: 37170377google scholar: lookup
  2. Madkour FA, Abdellatif AM, Osman YA, Kandyel RM. Histological and ultrastructural characterization of the dorso-ventral skin of the juvenile and the adult starry puffer fish (Arothron stellatus, Anonymous 1798). BMC Vet Res 2023 Oct 24;19(1):221.
    doi: 10.1186/s12917-023-03784-0pubmed: 37875870google scholar: lookup
Subscribe to our newsletter
Join 99,911 horse owners like you who receive our email updates on horse health and nutrition.