Scintigraphic tracking of mesenchymal stem cells after intravenous regional limb perfusion and subcutaneous administration in the standing horse.
Abstract: To assess distribution, uptake, and persistence of radiolabeled mesenchymal stem cells (MSC) using scintigraphy after intravenous regional limb perfusion (RLP) and subcutaneous injections in standing, sedated horses. Methods: Experimental study. Methods: Horses (n = 12). Methods: Six horses had RLP performed through the cephalic vein on 1 limb and subcutaneous injection in the metacarpal area in the opposite limb. The other 6 horses had RLP performed through the lateral palmar digital vein and subcutaneous injection in the coronary band. A pneumatic tourniquet was used for the RLP. MSC were labeled with technetium-HMPAO. Scintigraphic images were obtained at the time of injection, 1, 6, and 24 hours later. Results of RLP were compared with results from previous studies where similar injections were performed in anesthetized horses. Results: Both RLP techniques led to greater variability, lower uptake, lower persistence, and poorer distribution when compared to results previously reported for horses under general anesthesia. The subcutaneous injections in the metacarpal area and coronary band resulted in MSC loss to the general circulation but no evidence of local migration. Conclusions: Due to partial or complete failure of the tourniquet, RLP performed in the standing horse as described is less efficient than performed under general anesthesia. Further work is needed to optimize the use of tourniquets to perform RLP for MSC administration in standing patients. The subcutaneous injections did not result in local migration in these normal horses.
© Copyright 2014 by The American College of Veterinary Surgeons.
Publication Date: 2014-10-13 PubMed ID: 25307554DOI: 10.1111/j.1532-950X.2014.12289.xGoogle Scholar: Lookup
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- Clinical Trial
- Journal Article
Summary
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The research investigates the effectiveness and challenges of administering radiolabeled mesenchymal stem cells (MSCs) to horses. Techniques of injection included intravenous regional limb perfusion (RLP) and subcutaneous injections. The study found that the techniques used in standing, sedated horses were less effective compared to previously reported procedures performed on anesthetized horses.
Methods
The research involved an experiment with twelve horses. The horses were divided into two groups and each group was subjected to different sets of procedures:
- The first group of six horses underwent RLP through the cephalic vein of one limb and a subcutaneous injection in the metacarpal area of the other limb.
- The other group also had six horses. They underwent RLP through the lateral palmar digital vein of one limb and a subcutaneous injection in the coronary band area of the other limb.
MSCs were tagged with technetium-HMPAO for tracking via scintigraphic imaging. The images were acquired at the time of injection and one, six, and twenty-four hours later to analyze the trajectory, persistence, and distribution of the cells.
Results
The results were evaluated based on the uptake, persistence, variability, and distribution of the MSCs.
- The research found that both RLP techniques represented lower uptake, greater variability, poorer distribution, and lower persistence of MSCs.
- Results also indicated that the tourniquet used to perform RLP was either partially or wholly unsuccessful.
- The RLP performed on standing, sedated horses proved less efficient than procedures executed under general anesthesia.
Additionally, subcutaneous injections in the metacarpal area and coronary band resulted in a loss of MSCs to the general blood circulation, but showed no evidence of local migration of MSCs.
Conclusions
The research concluded that further study is needed to optimize the use of tourniquets in performing RLP for MSC administration in sedated, standing horses. Particularly, the issue of tourniquet failure needs to be addressed. Moreover, subcutaneous injections did not yield local migration of MSCs, a factor that needs to be looked into when considering potential applications of these methods.
Cite This Article
APA
Spriet M, Buerchler S, Trela JM, Hembrooke TA, Padgett KA, Rick MC, Vidal MA, Galuppo LD.
(2014).
Scintigraphic tracking of mesenchymal stem cells after intravenous regional limb perfusion and subcutaneous administration in the standing horse.
Vet Surg, 44(3), 273-280.
https://doi.org/10.1111/j.1532-950X.2014.12289.x Publication
Researcher Affiliations
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California Davis, Davis, California.
MeSH Terms
- Anesthesia, General / veterinary
- Animals
- Female
- Hoof and Claw / blood supply
- Horses
- Infusions, Intravenous / veterinary
- Injections, Subcutaneous / veterinary
- Male
- Mesenchymal Stem Cells
- Posture
- Radionuclide Imaging / veterinary
- Radiopharmaceuticals / pharmacology
- Technetium Tc 99m Exametazime / pharmacology
- Tourniquets / veterinary
Citations
This article has been cited 6 times.- Aldrich ED, Cui X, Murphy CA, Lim KS, Hooper GJ, McIlwraith CW, Woodfield TBF. Allogeneic mesenchymal stromal cells for cartilage regeneration: A review of in vitro evaluation, clinical experience, and translational opportunities. Stem Cells Transl Med 2021 Nov;10(11):1500-1515.
- Beerts C, Brondeel C, Pauwelyn G, Depuydt E, Tack L, Duchateau L, Xu Y, Saunders JH, Peremans K, Spaas JH. Scintigraphic tracking of (99m)Technetium-labelled equine peripheral blood-derived mesenchymal stem cells after intravenous, intramuscular, and subcutaneous injection in healthy dogs. Stem Cell Res Ther 2021 Jul 13;12(1):393.
- Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Front Bioeng Biotechnol 2020;8:972.
- Grady ST, Britton L, Hinrichs K, Nixon AJ, Watts AE. Persistence of fluorescent nanoparticle-labelled bone marrow mesenchymal stem cells in vitro and after intra-articular injection. J Tissue Eng Regen Med 2019 Feb;13(2):191-202.
- Kanazawa M, Atsuta I, Ayukawa Y, Yamaza T, Kondo R, Matsuura Y, Koyano K. The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model. Int J Implant Dent 2018 Jan 13;4(1):2.
- Berner D, Brehm W, Gerlach K, Gittel C, Offhaus J, Paebst F, Scharner D, Burk J. Longitudinal Cell Tracking and Simultaneous Monitoring of Tissue Regeneration after Cell Treatment of Natural Tendon Disease by Low-Field Magnetic Resonance Imaging. Stem Cells Int 2016;2016:1207190.
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