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Equine veterinary journal2021; 54(4); 662-669; doi: 10.1111/evj.13501

Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids.

Abstract: Bovine papillomavirus types 1 and 2 (BPV1 and BPV2) are accepted aetiological agents of equine sarcoids. Recently, genetically similar BPV13 has been identified from equine sarcoids in Brazil. Objective: To determine whether BPV13 DNA can be also found in sarcoid-affected horses in Austria, and donkeys in Northern Italy and the UK, and should hence be considered in the context of vaccine-mediated sarcoid prevention. Methods: Cross sectional study. Methods: A total of 194 archival, equine and asinine DNA isolates derived from confirmedly delta-BPV-positive tumours were subjected to quality control by photometric analysis and equine beta-actin PCR. Isolates with DNA concentrations >0.9 ng/µl and confirmed PCR-compatibility (n = 135) were subsequently screened for the presence of BPV13 DNA using BPV13-specific PCR primers for amplification of a 771 bp region comprising the BPV13 E5 gene. Results: BPV13 E5 PCR scored negative for all 135 samples. Included positive, negative and no-template controls yielded anticipated results, thus confirming reliability of obtained data. Conclusions: Moderate number of tested tumour DNA extracts (n = 135; equivalent to 127 tumour-affected equids). Conclusions: Despite its moderate size, the sample was considered representative enough to suggest a low occurrence of BPV13 in Austria, as it randomly comprised equine patients of different breed, age, gender, and European provenience. BPV13 was not associated with tested sarcoids in rescued donkeys originating from several other European countries. Large-scale BPV13 screenings are necessary to allow for a more precise estimation of the prevalence and distribution of BPV13 infections in European equids suffering from sarcoid disease.
© 2021 The Authors. Equine Veterinary Journal published by John Wiley & Sons Ltd on behalf of EVJ Ltd.
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Publication Date: 2021-08-30 PubMed ID: 34459020PubMed Central: PMC9292424DOI: 10.1111/evj.13501Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper investigates the presence of Bovine papillomavirus type 13 (BPV13) in a European population of horse and donkey sarcoids. The study concluded that BPV13 was not present in any of the tested samples, suggesting a low occurrence in Austria, and among rescued donkeys from several other European countries.

Research Objectives and Methods

The research focused on determining whether DNA belonging to BPV13 was present in horse and donkey sarcoid patients in Austria, Northern Italy, and the UK. This would be important in understanding whether BPV13 should be considered a factor in the development of vaccines to prevent sarcoids.

  • Researchers conducted cross-sectional study, using a total of 194 DNA isolates extracted from tumors definitively positive for delta-BPV.
  • These isolates were first subjected to quality control using a method called photometric analysis and equine beta-actin PCR.
  • Out of these, 135 isolates that had DNA concentration greater than 0.9 ng/µl and had confirmed PCR-compatibility were selected for the main component of the research.
  • A specific technique was used to detect the presence of BPV13 DNA in these 135 samples.

Findings of the Study

The research yielded negative results for the presence of BPV13 in all 135 tested samples. This conclusion was based on:

  • The use of a specific PCR primers for amplification of a region of the BPV13 E5 gene which was used to determine the presence of BPV13.
  • Positive, negative, and no-template controls used in the experiment gave the expected outcomes, supporting the reliability of the data.

Conclusions and Recommendations

Despite the moderate sample size, the research concluded that:

  • There could be a low occurrence of BPV13 in Austria among different horse breeds, ages, genders, and provenience, based on the random selection of samples.
  • BPV13 was not associated with the tested sarcoids in rescued donkeys originating from a number of European countries.
  • The researchers, however, recommended larger scale BPV13 screenings to provide a more accurate estimation of its prevalence and distribution among European equids suffering from sarcoid disease.

Cite This Article

APA
Jindra C, Kamjunke AK, Jones S, Brandt S. (2021). Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids. Equine Vet J, 54(4), 662-669. https://doi.org/10.1111/evj.13501

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 54
Issue: 4
Pages: 662-669

Researcher Affiliations

Jindra, Christoph
  • Research Group Oncology (RGO), Clinical Unit of Equine Surgery, University Clinic for Horses, University of Veterinary Medicine, Vienna, Austria.
Kamjunke, Ann-Kristin
  • Research Group Oncology (RGO), Clinical Unit of Equine Surgery, University Clinic for Horses, University of Veterinary Medicine, Vienna, Austria.
Jones, Sarah
  • School of Veterinary Medicine, University of Glasgow, Scotland, UK.
Brandt, Sabine
  • Research Group Oncology (RGO), Clinical Unit of Equine Surgery, University Clinic for Horses, University of Veterinary Medicine, Vienna, Austria.

Conflict of Interest Statement

No competing interests have been declared.

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Citations

This article has been cited 5 times.
  1. Jindra C, Hainisch EK, Brandt S. Immunotherapy of Equine Sarcoids-From Early Approaches to Innovative Vaccines. Vaccines (Basel) 2023 Mar 30;11(4).
    doi: 10.3390/vaccines11040769pubmed: 37112681google scholar: lookup
  2. Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids. Animals (Basel) 2021 Oct 29;11(11).
    doi: 10.3390/ani11113093pubmed: 34827825google scholar: lookup
  3. Cutarelli A, Buonavoglia A, Fusco G, Pellicanò R, Napoletano M, Brandt S, Roperto S. Accurate identification of bovine deltapapillomavirus in equine sarcoids by ddPCR. Sci Rep 2025 Aug 11;15(1):29414.
    doi: 10.1038/s41598-025-15353-6pubmed: 40790360google scholar: lookup
  4. De Falco F, Cutarelli A, Pellicanò R, Brandt S, Roperto S. Molecular Detection and Quantification of Ovine Papillomavirus DNA in Equine Sarcoid. Transbound Emerg Dis 2024;2024:6453158.
    doi: 10.1155/2024/6453158pubmed: 40303025google scholar: lookup
  5. Cutarelli A, De Falco F, Serpe F, Izzo S, Fusco G, Catoi C, Roperto S. Ultrasensitive detection and quantification of bovine Deltapapillomavirus in the semen of healthy horses. Sci Rep 2025 Jan 4;15(1):769.
    doi: 10.1038/s41598-024-81682-7pubmed: 39755719google scholar: lookup
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