Season does not influence embryo recovery rate and conceptus size until day 14 after ovulation in the horse.
Abstract: Although the horse is a seasonal breeding species, a considerable number of mares continue to cycle throughout autumn and winter. Slower equine embryo growth during the non-breeding season has been hypothesized, and because smaller embryo size is beneficial for cryopreservation, embryo collection outside the breeding season could be an interesting approach for the production of frozen horse embryos. In the present retrospective study, we have therefore analysed embryo recovery rates and conceptus size in mares (n = 30) throughout the year. Conceptus diameter was either size determined after collection with a microscopic scale (day 7-10 after ovulation) or determined by transrectal ultrasound immediately before collection (day 11-14 after ovulation). In 19 of the 30 mares (63%), ovulatory cycles were detected throughout the year. A total of 352 embryo collections with a mean recovery rate of 64.2% were performed and not affected by season. The size was analysed in a total of 165 conceptuses. Conceptus diameter significantly increased (p < 0.001) with day of pregnancy (e.g. day 7: 0.3 ± 0.04, day 10: 4.1 ± 0.2, day 12: 10.1 ± 0.5, day 14: 17.4 ± 0.9 mm), but was not influenced by season. In conclusion, successful embryo collection is possible throughout the year in spontaneously cyclic mares. Under these conditions, neither collection rates nor embryo growth appeared to be affected by season.
© 2015 Blackwell Verlag GmbH.
Publication Date: 2015-01-27 PubMed ID: 25626461DOI: 10.1111/rda.12490Google Scholar: Lookup
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- Journal Article
Summary
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The researchers conducted a retrospective study that showed embryo recovery rates and the size of the conceptus in horses didn’t vary with seasons.
Research Background
- The paper addresses the seasonal breeding nature of horses and the hypothesis that horse embryo growth could be slower during the non-breeding season.
- Since smaller embryo sizes are beneficial for cryopreservation, collecting embryos outside the breeding season could be productive for freezing horse embryos.
Methodology
- The research was conducted on 30 mares over different seasons of the year.
- The size of the conceptus diameter was determined after collection using a microscopic scale (day 7-10 after ovulation) or through transrectal ultrasound before collection (day 11-14 after ovulation).
- In total, 352 embryo collections were performed and analysed.
Findings
- The study found that a total of 63% of the mares cycled throughout the year, regardless of the season.
- The mean recovery rate of embryos was 64.2%, and it was not influenced by the season.
- The size of the conceptus also didn’t change with seasons, however, it significantly increased with each day of pregnancy.
Conclusion
- Overall, the study concludes that it’s possible to successfully collect horse embryos all year round in spontaneously cyclic mares.
- Additionally, the study dispels the hypothesis of slower embryo growth in the non-breeding season, finding that neither collection rates nor embryo growth appeared to be affected by the season.
Cite This Article
APA
Aurich C, Budik S.
(2015).
Season does not influence embryo recovery rate and conceptus size until day 14 after ovulation in the horse.
Reprod Domest Anim, 50(2), 299-303.
https://doi.org/10.1111/rda.12490 Publication
Researcher Affiliations
- Centre for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Austria.
- Centre for Artificial Insemination and Embryo Transfer, Vetmeduni Vienna, Austria.
MeSH Terms
- Animals
- Embryo, Mammalian / physiology
- Embryonic Development / physiology
- Female
- Horses / embryology
- Horses / physiology
- Ovulation / physiology
- Pregnancy
- Seasons
- Tissue and Organ Harvesting / veterinary
Citations
This article has been cited 1 times.- Aurich C, Budik S. Early pregnancy in the horse revisited - does exception prove the rule?. J Anim Sci Biotechnol 2015;6:50.
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