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Home / Equine Research Bank / Theriogenology / Seminal plasma affects membrane integrity and motility of eq...
Theriogenology1996; 46(5); 791-797; doi: 10.1016/s0093-691x(96)00237-3

Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation.

Abstract: Effects of seminal plasma on post-thaw motility and membrane integrity of cryopreserved horse spermatozoa were investigated. Carboxyfluorescein diacetate staining was used for the assessment of sperm membrane integrity. Adding 30% of seminal plasma from stallions with high post-thaw sperm motility to ejaculates from stallions with low post-thaw sperm motility increased progressive motility from 24.0 +/- 1.6 to 34.5 +/- 1.9% (P < 0.05) and membrane integrity from 27.0 +/- 2.1 to 34.3 +/- 2.3% membrane-intact spermatozoa (P < 0.05). Conversely, the addition of seminal plasma from stallions with low post-thaw sperm motility to ejaculates from stallions with high post-thaw motility decreased progressive motility from 36.0 +/- 1.6 to 30.0 +/- 2.7% (P < 0.05) but did not induce changes in membrane integrity. Seminal plasma from stallions with opposite post-thaw motility therefore clearly influenced the resistance of spermatozoa to the freezing and thawing process. We conclude that the individual composition of seminal plasma affects the suitability of stallions for semen cryopreservation.
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Publication Date: 1996-10-01 PubMed ID: 16727943DOI: 10.1016/s0093-691x(96)00237-3Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates the effects of seminal plasma on the movement and membrane integrity of horse spermatozoa after it has been frozen and thawed. The research finds that the individual makeup of the seminal plasma can influence how stallion semen withstands the process of freezing and thawing.

Objective of the Study and Methodology

  • The aim of the research was to examine how seminal plasma impacts the motility (movement) and membrane integrity of cryopreserved horse spermatozoa (frozen and subsequently thawed sperm).
  • The researchers adopted carboxyfluorescein diacetate staining as a tool to evaluate the membrane integrity of the sperm.

Key Findings

  • The study discovered that when 30% of seminal plasma taken from stallions with superior post-thaw sperm motility was added to ejaculates from stallions with inferior post-thaw sperm motility, there was a significant improvement in the progressive motility and membrane integrity of the sperm.
  • Conversely, the introduction of seminal plasma from stallions with low post-thaw sperm motility to ejaculates from stallions with high post-thaw sperm motility showed a significant reduction in progressive motility. Nevertheless, no change was observed in the membrane integrity.
  • From these observations, the researchers concluded that seminal plasma from stallions with contrasting post-thaw motility evidently influences the resistance of the sperm to the freezing and thawing process.

Conclusion

  • The research concluded that the individual composition of seminal plasma impacts the ability of stallion semen for cryopreservation.
  • In other words, the specific mixture of seminal plasma in the sperm plays a crucial role in determining how well horse sperm can withstand the process of being frozen and thawed.

Cite This Article

APA
Aurich JE, Kühne A, Hoppe H, Aurich C. (1996). Seminal plasma affects membrane integrity and motility of equine spermatozoa after cryopreservation. Theriogenology, 46(5), 791-797. https://doi.org/10.1016/s0093-691x(96)00237-3

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 46
Issue: 5
Pages: 791-797

Researcher Affiliations

Aurich, J E
  • Clinic for Bovine Obstetrics and Gynecology, College of Veterinary Medicine, 30173 Hannover, Germany.
Kühne, A
    Hoppe, H
      Aurich, C

        Citations

        This article has been cited 13 times.
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          doi: 10.3389/fvets.2023.1132373pubmed: 36968471google scholar: lookup
        2. Rickard JP, Pool K, de Graaf SP, Portas T, Rourke N, Wiesner M, Hildebrandt TB, Göritz F, Hermes R. Increasing the Yield and Cryosurvival of Spermatozoa from Rhinoceros Ejaculates Using the Enzyme Papain.. Biology (Basel) 2022 Jan 18;11(2).
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        3. Catalán J, Yánez-Ortiz I, Tvarijonaviciute A, González-Arostegui LG, Rubio CP, Yeste M, Miró J, Barranco I. Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance.. Antioxidants (Basel) 2022 Feb 18;11(2).
          doi: 10.3390/antiox11020417pubmed: 35204299google scholar: lookup
        4. Cheng Y, Franěk R, Rodina M, Xin M, Cosson J, Zhang S, Linhart O. Optimization of Sperm Management and Fertilization in Zebrafish (Danio rerio (Hamilton)).. Animals (Basel) 2021 May 27;11(6).
          doi: 10.3390/ani11061558pubmed: 34071823google scholar: lookup
        5. Toledano-Díaz A, Castaño C, Velázquez R, Bóveda P, López-Sebastián A, Martínez-Nevado E, Villaverde-Morcillo S, Esteso MC, Santiago-Moreno J. Cryopreservation of ferret (Mustela putorius furo) sperm collected by rectal massage and electroejaculation: Comparison of a decelerating and an accelerating freezing rate protocol.. Vet Med Sci 2021 Jan;7(1):256-263.
          doi: 10.1002/vms3.362pubmed: 33040487google scholar: lookup
        6. Bubenickova F, Postlerova P, Simonik O, Sirohi J, Sichtar J. Effect of Seminal Plasma Protein Fractions on Stallion Sperm Cryopreservation.. Int J Mol Sci 2020 Sep 3;21(17).
          doi: 10.3390/ijms21176415pubmed: 32899253google scholar: lookup
        7. Aurich J, Kuhl J, Tichy A, Aurich C. Efficiency of Semen Cryopreservation in Stallions.. Animals (Basel) 2020 Jun 13;10(6).
          doi: 10.3390/ani10061033pubmed: 32545785google scholar: lookup
        8. Papas M, Catalán J, Fernandez-Fuertes B, Arroyo L, Bassols A, Miró J, Yeste M. Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance.. Antioxidants (Basel) 2019 Nov 9;8(11).
          doi: 10.3390/antiox8110539pubmed: 31717586google scholar: lookup
        9. Šichtař J, Bubeníčková F, Sirohi J, Šimoník O. How to Increase Post-Thaw Semen Quality in Poor Freezing Stallions: Preliminary Results of the Promising Role of Seminal Plasma Added after Thawing.. Animals (Basel) 2019 Jul 3;9(7).
          doi: 10.3390/ani9070414pubmed: 31277261google scholar: lookup
        10. Domingo P, Olaciregui M, González N, De Blas I, Gil L. Effects of seminal plasma and different cryoprotectants on rabbit sperm preservation at 16°C.. Exp Anim 2018 Nov 1;67(4):413-420.
          doi: 10.1538/expanim.17-0152pubmed: 29899185google scholar: lookup
        11. Morrell JM, Johannisson A. Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation.. J Vet Med 2014;2014:325451.
          doi: 10.1155/2014/325451pubmed: 26464926google scholar: lookup
        12. Kaeoket K, Chanapiwat P, Tummaruk P, Techakumphu M, Kunavongkrit A. A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility.. Trop Anim Health Prod 2011 Jun;43(5):1049-55.
          doi: 10.1007/s11250-011-9804-6pubmed: 21347830google scholar: lookup
        13. Cary JA, Madill S, Farnsworth K, Hayna JT, Duoos L, Fahning ML. A comparison of electroejaculation and epididymal sperm collection techniques in stallions.. Can Vet J 2004 Jan;45(1):35-41.
          pubmed: 14992252
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