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Antioxidants (Basel, Switzerland)2022; 11(7); 1279; doi: 10.3390/antiox11071279

Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse.

Abstract: The objective of this study was to determine the relationship of enzymatic (superoxide dismutase, SOD; glutathione peroxidase, GPX; catalase, CAT; and paraoxonase type 1, PON1) and non-enzymatic antioxidants (measured in terms of: Trolox equivalent antioxidant capacity, TEAC; cupric-reducing antioxidant capacity, CUPRAC; and ferric-reducing ability of plasma, FRAP), as well as the oxidative stress index (OSI) in seminal plasma (SP) with the resilience of horse sperm to freeze-thawing. Twenty-one ejaculates (one per individual) were collected and split into two aliquots: the first was used to harvest the SP and assess the activity levels of antioxidants and the OSI, and the second one was cryopreserved. The following post-thaw sperm quality parameters were evaluated: sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, intracellular levels of reactive oxygen species (ROS), and plasma membrane lipid disorder. Based on post-thaw total motility (TM) and plasma membrane integrity (SYBR14+/PI−), ejaculates were hierarchically (p < 0.001) clustered into two groups of good (GFE) and poor (PFE) freezability. The SP activity levels of PON1, SOD, and TEAC were higher (p < 0.05) in GFE than in PFE, showing a positive relationship (p < 0.05) with some sperm motility parameters and with plasma membrane (PON1 and TEAC) and acrosome (SOD and TEAC) integrity. In contrast, OSI was higher (p < 0.05) in the SP of PFE than in that of GFE, and was negatively correlated (p < 0.05) to some sperm motility parameters and to plasma membrane and acrosome integrity, and positively (p < 0.05) to the percentage of viable sperm with high plasma membrane lipid disorder. In conclusion, enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants of SP are related to horse sperm cryotolerance. In addition, our results suggest that PON1 could be one of the main antioxidant enzymes involved in the control of ROS in this species. Further investigation is needed to confirm the potential use of these SP-antioxidants and OSI to predict sperm cryotolerance in horses.
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Publication Date: 2022-06-28 PubMed ID: 35883774PubMed Central: PMC9311553DOI: 10.3390/antiox11071279Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The main objective of the research was to ascertain the connection between certain antioxidants and oxidative stress index present in the seminal plasma of horses and their influence on the sperm’s resilience to freeze-thawing – a process used in fertility treatments.

Initial Study Process

  • The researchers collected semen from 21 individual horses, which they divided into two aliquots. One aliquot was used to obtain the seminal plasma (SP) in which they measured the enzyme activity levels of particular antioxidants (superoxide dismutase, paraoxonase type 1, glutathione peroxidase, and catalase) and the oxidative stress index (OSI). The other aliquot of semen was cryopreserved or frozen.

Assessing Sperm Quality After Cryopreservation

  • After thawing the cryopreserved semen, the team then evaluated the following sperm quality parameters: overall sperm motility, plasma membrane and acrosome integrity, mitochondrial membrane potential, levels of reactive oxygen species within the cells, and the plasma membrane lipid disorder.

Comparison of Sperm Freezability

  • The scientists classified the thawed semen samples into two categories (poor “PFE” and good “GFE” freezability) based on their overall motility and plasma membrane integrity.
  • They discovered that the activity levels of some antioxidants (PON1, SOD, and TEAC) in the SP were significantly higher in the GFE group than in the PFE group.
  • Furthermore, these antioxidants also showed a positive correlation with certain sperm motility parameters and the plasma and acrosome membranes’ integrity.
  • On the contrary, the OSI found in the SP was greater in the PFE than in the GFE, implying a negative correlation with some sperm motility parameters and plasma and acrosome membrane’s integrity but a positive correlation with the percentage of viable sperm with high plasma membrane lipid disorder.

Conclusions and Future Research

  • The findings suggest that both enzymatic (PON1 and SOD) and non-enzymatic (TEAC) antioxidants present in the SP influence the sperm’s resilience to freezing and thawing in horses, with PON1 potentially playing a key role in managing reactive oxygen species in this species.
  • However, more research is necessary to validate these findings and determine if these specific antioxidants and the oxidative stress index could predict sperm resilience to the freezing-thawing process in horses, which could have significant implications for fertility treatments.

Cite This Article

APA
Catalán J, Yánez-Ortiz I, Tvarijonaviciute A, González-Aróstegui LG, Rubio CP, Barranco I, Yeste M, Miró J. (2022). Seminal Plasma Antioxidants Are Related to Sperm Cryotolerance in the Horse. Antioxidants (Basel), 11(7), 1279. https://doi.org/10.3390/antiox11071279

Publication

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
NlmUniqueID: 101668981
Country: Switzerland
Language: English
Volume: 11
Issue: 7
PII: 1279

Researcher Affiliations

Catalán, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, ES-17003 Girona, Spain.
  • Faculty of Veterinary Medicine, University of Teramo, Loc. Piano d'Accio, IT-64100 Teramo, Italy.
Yánez-Ortiz, Iván
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, ES-17003 Girona, Spain.
Tvarijonaviciute, Asta
  • Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, ES-30100 Murcia, Spain.
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
González-Aróstegui, Luis Guillermo
  • Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, ES-30100 Murcia, Spain.
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
Rubio, Camila P
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
  • Department of Animal and Food Science, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
Barranco, Isabel
  • Department of Veterinary Medical Sciences, University of Bologna, IT-40064 Ozzano dell'Emilia, Italy.
Yeste, Marc
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, ES-17003 Girona, Spain.
  • Catalan Institution for Research and Advanced Studies (ICREA), ES-08010 Barcelona, Spain.
Miró, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 10 times.
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