Sequence variants of bovine papillomavirus E5 detected in equine sarcoids.
Abstract: The equine sarcoid, one of the most common dermatological lesions in equids, is a benign, locally invasive dermal fibroblastic lesion. Previous studies have suggested an association with two bovine papilloma virus (BPV) types, BPV-1 and BPV-2. In the present study, we examined sarcoids from horses from two geographical areas, Switzerland and the UK, for the major transforming gene of BPV, E5. We detected BPV DNA for the E5 open reading frame and viral E5 RNA transcripts in most sarcoids. Sequence analysis of the E5 open reading frame of sarcoid-associated BPV detected several unique DNA sequence variants, three of which resulted in sarcoid specific amino acid sequence variations. It is unclear if these sequence variants contribute to the unique clinical presentation of the sarcoid. However, our work provides further evidence of the association between BPV and sarcoid development and the direct involvement of the virus in the pathogenesis of sarcoids.
Publication Date: 2003-09-03 PubMed ID: 12951274DOI: 10.1016/s0168-1702(03)00175-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research study delves into the relationship between certain sequence variants of bovine papillomavirus E5 and equine sarcoids, common skin lesions found in equids. The researchers found unique DNA sequence variants in sarcoid-associated bovine papillomavirus, providing further evidence of the virus’s involvement in sarcoid pathogenesis.
Objectives and Methodology of the Study
- This study aimed to explore the association between bovine papillomavirus (BPV), particularly the E5 gene, and equine sarcoids, which are benign yet invasive skin lesions common in equids.
- Prior research suggested a relationship between equine sarcoids and two types of BPV, BPV-1 and BPV-2. This current study seeks to expand on this understanding.
- Sarcoids from horses in the UK and Switzerland formed the basis of the study with focus on detecting the presence of the E5 gene of BPV.
- The researchers carried out a sequence analysis of the E5 open reading frame from sarcoid-associated BPV.
Findings of the Study
- Most sarcoids were found to contain both BPV DNA for the E5 open reading frame (the portion of a gene that is read by the ribosome during protein synthesis) and viral E5 RNA transcripts. This supports the inference of the significant involvement of the virus in the development of sarcoids.
- Several unique DNA sequence variants were detected from the E5 open reading frame of sarcoid-associated BPV.
- Three of these variants resulted in specific amino acid sequence variations in the sarcoids, pointing to potential contributions to the unique clinical presentation of the sarcoids. Nevertheless, the definitive role these sequence variants play remains unclear.
Significance of the Study
- This study validates the association between BPV and the development of equine sarcoids.
- The observation of unique DNA sequence variants in sarcoid-associated BPV suggests the direct involvement of the virus in the pathogenesis of sarcoids.
- The findings contribute to a clearer understanding of the nature of equine sarcoids and their etiology, which may open avenues for more effective therapeutic interventions.
Cite This Article
APA
Chambers G, Ellsmore VA, O'Brien PM, Reid SW, Love S, Campo MS, Nasir L.
(2003).
Sequence variants of bovine papillomavirus E5 detected in equine sarcoids.
Virus Res, 96(1-2), 141-145.
https://doi.org/10.1016/s0168-1702(03)00175-8 Publication
Researcher Affiliations
- Department of Veterinary Pathology, Institute of Comparative Medicine, University of Glasgow Veterinary School, Garscube Estate, Glasgow G611QH, UK. g.chambers@vet.gla.ac.uk
MeSH Terms
- Animals
- Bovine papillomavirus 1 / classification
- Bovine papillomavirus 1 / genetics
- DNA, Viral / analysis
- DNA, Viral / genetics
- Genetic Variation
- Horse Diseases / virology
- Horses
- Molecular Sequence Data
- Papillomavirus Infections / epidemiology
- Papillomavirus Infections / veterinary
- Papillomavirus Infections / virology
- Skin Diseases / epidemiology
- Skin Diseases / veterinary
- Skin Diseases / virology
Citations
This article has been cited 12 times.- Munday JS, Orbell G, Fairley RA, Hardcastle M, Vaatstra B. Evidence from a Series of 104 Equine Sarcoids Suggests That Most Sarcoids in New Zealand Are Caused by Bovine Papillomavirus Type 2, although Both BPV1 and BPV2 DNA Are Detectable in around 10% of Sarcoids.. Animals (Basel) 2021 Oct 29;11(11).
- Jindra C, Hainisch EK, Rümmele A, Wolschek M, Muster T, Brandt S. Influenza virus vector iNS1 expressing bovine papillomavirus 1 (BPV1) antigens efficiently induces tumour regression in equine sarcoid patients.. PLoS One 2021;16(11):e0260155.
- Jindra C, Kamjunke AK, Jones S, Brandt S. Screening for bovine papillomavirus type 13 (BPV13) in a European population of sarcoid-bearing equids.. Equine Vet J 2021 Aug 30;54(4):662-9.
- Willemsen A, Bravo IG. Origin and evolution of papillomavirus (onco)genes and genomes.. Philos Trans R Soc Lond B Biol Sci 2019 May 27;374(1773):20180303.
- Araldi RP, Assaf SMR, Carvalho RF, Carvalho MACR, Souza JM, Magnelli RF, Módolo DG, Roperto FP, Stocco RC, Beçak W. Papillomaviruses: a systematic review.. Genet Mol Biol 2017 Jan-Mar;40(1):1-21.
- Wilson AD, Hicks C. Both tumour cells and infiltrating T-cells in equine sarcoids express FOXP3 associated with an immune-supressed cytokine microenvironment.. Vet Res 2016 May 9;47(1):55.
- Martano M, Corteggio A, Restucci B, De Biase ME, Borzacchiello G, Maiolino P. Extracellular matrix remodeling in equine sarcoid: an immunohistochemical and molecular study.. BMC Vet Res 2016 Feb 2;12:24.
- Altamura G, Corteggio A, Nasir L, Yuan ZQ, Roperto F, Borzacchiello G. Analysis of activated platelet-derived growth factor β receptor and Ras-MAP kinase pathway in equine sarcoid fibroblasts.. Biomed Res Int 2013;2013:283985.
- Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids.. J Clin Microbiol 2013 Jul;51(7):2167-71.
- Corteggio A, Altamura G, Roperto F, Borzacchiello G. Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?. Infect Agent Cancer 2013 Jan 9;8(1):1.
- Wobeser BK, Davies JL, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL. Epidemiology of equine sarcoids in horses in western Canada.. Can Vet J 2010 Oct;51(10):1103-8.
- Yuan ZQ, Nicolson L, Marchetti B, Gault EA, Campo MS, Nasir L. Transcriptional changes induced by bovine papillomavirus type 1 in equine fibroblasts.. J Virol 2008 Jul;82(13):6481-91.
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