FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Vet Med Sci / Seroepidemiology of non-primate hepacivirus (NPHV) in Japane...
The Journal of veterinary medical science2017; 80(1); 186-189; doi: 10.1292/jvms.17-0527

Seroepidemiology of non-primate hepacivirus (NPHV) in Japanese native horses.

Abstract: Non-primate hepacivirus (NPHV) is recently identified as a closely related homologue of hepatitis C virus. The previous studies showed a high prevalence of NPHV infection among Japanese domestic horses originated from abroad. The historical distribution of NPHV among horses in Japan, therefore, is still unknown. In this study, seroepidemiological study of NPHV was conducted using 335 sera from five breeds of Japanese native horses. These horses are maintained as the pedigree and are reared apart from other horse breeds. The detection of antibodies against NPHV were conducted by western blot analysis using the recombinant protein of the NPHV core protein. The antibodies against NPHV were detected in all five breeds, 83 out of 335 (23.4%) horses. These results suggested that NPHV was circulating among Japanese native horses.
Get Full Text
Publication Date: 2017-11-29 PubMed ID: 29187712PubMed Central: PMC5797880DOI: 10.1292/jvms.17-0527Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents a study on the seroepidemiology of non-primate hepacivirus (NPHV) in Japanese native horse breeds.

Objective of the Research

  • The study aimed to investigate the prevalence and historical distribution of NPHV among native horses in Japan.

Methodology of the Research

  • The researchers used 335 sera samples from five breeds of Japanese native horses, all of which are kept separate from other horse breeds and conserved as distinctive bloodlines.
  • They checked for antibodies against NPHV in these samples. This was accomplished using a diagnostic method known as Western Blot analysis. The process utilizes a recombinant protein of the NPHV core protein.

Findings of the Research

  • The research found that NPHV antibodies were present in all five horse breeds tested.
  • From the 335 horses tested, 83 of them (or approximately 23.4%) were found to be positive for antibodies against NPHV.
  • This result suggested that NPHV has been circulating among these native Japanese horses.

Implication of the Findings

  • The discovery of NPHV in native horse breeds differs from previous studies, which suggested a high prevalence of NPHV infection among Japanese domestic horses imported from abroad.
  • Hence, the study offers new insights into the distribution and prevalence of NPHV in Japanese horse populations, however the broader historical distribution of NPHV among horses in Japan remains unclear based on this study alone.

Cite This Article

APA
Hayashi S, Tanaka T, Moriishi K, Hirayama K, Yamada A, Hotta K. (2017). Seroepidemiology of non-primate hepacivirus (NPHV) in Japanese native horses. J Vet Med Sci, 80(1), 186-189. https://doi.org/10.1292/jvms.17-0527

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 80
Issue: 1
Pages: 186-189

Researcher Affiliations

Hayashi, Shizuka
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Tanaka, Tomohisa
  • Department of Microbiology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan.
Moriishi, Kohji
  • Department of Microbiology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, Yamanashi 409-3898, Japan.
Hirayama, Kazuhiro
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Yamada, Akio
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Hotta, Kozue
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

MeSH Terms

  • Animals
  • Female
  • Hepacivirus
  • Hepatitis C / epidemiology
  • Hepatitis C / veterinary
  • Hepatitis C / virology
  • Horse Diseases / epidemiology
  • Horse Diseases / virology
  • Horses / virology
  • Japan / epidemiology
  • Male
  • Prevalence
  • Seroepidemiologic Studies

References

This article includes 21 references
  1. Baechlein C, Fischer N, Grundhoff A, Alawi M, Indenbirken D, Postel A, Baron AL, Offinger J, Becker K, Beineke A, Rehage J, Becher P. Identification of a Novel Hepacivirus in Domestic Cattle from Germany.. J Virol 2015 Jul;89(14):7007-15.
    doi: 10.1128/JVI.00534-15pmc: PMC4473572pubmed: 25926652google scholar: lookup
  2. Bukh J, Apgar CL, Govindarajan S, Purcell RH. Host range studies of GB virus-B hepatitis agent, the closest relative of hepatitis C virus, in New World monkeys and chimpanzees.. J Med Virol 2001 Dec;65(4):694-7.
    doi: 10.1002/jmv.2092pubmed: 11745933google scholar: lookup
  3. Corman VM, Grundhoff A, Baechlein C, Fischer N, Gmyl A, Wollny R, Dei D, Ritz D, Binger T, Adankwah E, Marfo KS, Annison L, Annan A, Adu-Sarkodie Y, Oppong S, Becher P, Drosten C, Drexler JF. Highly divergent hepaciviruses from African cattle.. J Virol 2015 Jun;89(11):5876-82.
    doi: 10.1128/JVI.00393-15pmc: PMC4442428pubmed: 25787289google scholar: lookup
  4. Drexler JF, Corman VM, Müller MA, Lukashev AN, Gmyl A, Coutard B, Adam A, Ritz D, Leijten LM, van Riel D, Kallies R, Klose SM, Gloza-Rausch F, Binger T, Annan A, Adu-Sarkodie Y, Oppong S, Bourgarel M, Rupp D, Hoffmann B, Schlegel M, Kümmerer BM, Krüger DH, Schmidt-Chanasit J, Setién AA, Cottontail VM, Hemachudha T, Wacharapluesadee S, Osterrieder K, Bartenschlager R, Matthee S, Beer M, Kuiken T, Reusken C, Leroy EM, Ulrich RG, Drosten C. Evidence for novel hepaciviruses in rodents.. PLoS Pathog 2013;9(6):e1003438.
    doi: 10.1371/journal.ppat.1003438pmc: PMC3688547pubmed: 23818848google scholar: lookup
  5. Firth C, Bhat M, Firth MA, Williams SH, Frye MJ, Simmonds P, Conte JM, Ng J, Garcia J, Bhuva NP, Lee B, Che X, Quan PL, Lipkin WI. Detection of zoonotic pathogens and characterization of novel viruses carried by commensal Rattus norvegicus in New York City.. mBio 2014 Oct 14;5(5):e01933-14.
    doi: 10.1128/mBio.01933-14pmc: PMC4205793pubmed: 25316698google scholar: lookup
  6. Gather T, Walter S, Todt D, Pfaender S, Brown RJP, Postel A, Becher P, Moritz A, Hansmann F, Baumgaertner W, Feige K, Steinmann E, Cavalleri JV. Vertical transmission of hepatitis C virus-like non-primate hepacivirus in horses.. J Gen Virol 2016 Oct;97(10):2540-2551.
    doi: 10.1099/jgv.0.000561pubmed: 27461949google scholar: lookup
  7. Gemaque BS, Junior Souza de Souza A, do Carmo Pereira Soares M, Malheiros AP, Silva AL, Alves MM, Gomes-Gouvêa MS, Pinho JR, Ferreira de Figueiredo H, Ribeiro DB, Souza da Silva J, Moraes LA, Ribeiro AS, Pereira WL. Hepacivirus infection in domestic horses, Brazil, 2011-2013.. Emerg Infect Dis 2014 Dec;20(12):2180-2.
    doi: 10.3201/eid2012.140603pmc: PMC4257787pubmed: 25420101google scholar: lookup
  8. Kapoor A, Simmonds P, Gerold G, Qaisar N, Jain K, Henriquez JA, Firth C, Hirschberg DL, Rice CM, Shields S, Lipkin WI. Characterization of a canine homolog of hepatitis C virus.. Proc Natl Acad Sci U S A 2011 Jul 12;108(28):11608-13.
    doi: 10.1073/pnas.1101794108pmc: PMC3136326pubmed: 21610165google scholar: lookup
  9. Kapoor A, Simmonds P, Scheel TK, Hjelle B, Cullen JM, Burbelo PD, Chauhan LV, Duraisamy R, Sanchez Leon M, Jain K, Vandegrift KJ, Calisher CH, Rice CM, Lipkin WI. Identification of rodent homologs of hepatitis C virus and pegiviruses.. mBio 2013 Apr 9;4(2):e00216-13.
    doi: 10.1128/mBio.00216-13pmc: PMC3622934pubmed: 23572554google scholar: lookup
  10. Karayiannis P, McGarvey MJ. The GB hepatitis viruses.. J Viral Hepat 1995;2(5):221-6.
    doi: 10.1111/j.1365-2893.1995.tb00033.xpubmed: 8745313google scholar: lookup
  11. Lauck M, Sibley SD, Lara J, Purdy MA, Khudyakov Y, Hyeroba D, Tumukunde A, Weny G, Switzer WM, Chapman CA, Hughes AL, Friedrich TC, O'Connor DH, Goldberg TL. A novel hepacivirus with an unusually long and intrinsically disordered NS5A protein in a wild Old World primate.. J Virol 2013 Aug;87(16):8971-81.
    doi: 10.1128/JVI.00888-13pmc: PMC3754081pubmed: 23740998google scholar: lookup
  12. Lyons S, Kapoor A, Sharp C, Schneider BS, Wolfe ND, Culshaw G, Corcoran B, McGorum BC, Simmonds P. Nonprimate hepaciviruses in domestic horses, United kingdom.. Emerg Infect Dis 2012 Dec;18(12):1976-82.
    doi: 10.3201/eid1812.120498pmc: PMC3557883pubmed: 23171728google scholar: lookup
  13. Masatani T, Takashima Y, Takasu M, Matsuu A, Amaya T. Prevalence of anti-Toxoplasma gondii antibody in domestic horses in Japan.. Parasitol Int 2016 Apr;65(2):146-50.
    doi: 10.1016/j.parint.2015.11.006pubmed: 26593178google scholar: lookup
  14. Matsuu A, Hobo S, Ando K, Sanekata T, Sato F, Endo Y, Amaya T, Osaki T, Horie M, Masatani T, Ozawa M, Tsukiyama-Kohara K. Genetic and serological surveillance for non-primate hepacivirus in horses in Japan.. Vet Microbiol 2015 Sep 30;179(3-4):219-27.
    doi: 10.1016/j.vetmic.2015.05.028pubmed: 26070772google scholar: lookup
  15. Pfaender S, Walter S, Grabski E, Todt D, Bruening J, Romero-Brey I, Gather T, Brown RJ, Hahn K, Puff C, Pfankuche VM, Hansmann F, Postel A, Becher P, Thiel V, Kalinke U, Wagner B, Bartenschlager R, Baumgärtner W, Feige K, Pietschmann T, Cavalleri JM, Steinmann E. Immune protection against reinfection with nonprimate hepacivirus.. Proc Natl Acad Sci U S A 2017 Mar 21;114(12):E2430-E2439.
    doi: 10.1073/pnas.1619380114pmc: PMC5373355pubmed: 28275093google scholar: lookup
  16. Quan PL, Firth C, Conte JM, Williams SH, Zambrana-Torrelio CM, Anthony SJ, Ellison JA, Gilbert AT, Kuzmin IV, Niezgoda M, Osinubi MO, Recuenco S, Markotter W, Breiman RF, Kalemba L, Malekani J, Lindblade KA, Rostal MK, Ojeda-Flores R, Suzan G, Davis LB, Blau DM, Ogunkoya AB, Alvarez Castillo DA, Moran D, Ngam S, Akaibe D, Agwanda B, Briese T, Epstein JH, Daszak P, Rupprecht CE, Holmes EC, Lipkin WI. Bats are a major natural reservoir for hepaciviruses and pegiviruses.. Proc Natl Acad Sci U S A 2013 May 14;110(20):8194-9.
    doi: 10.1073/pnas.1303037110pmc: PMC3657805pubmed: 23610427google scholar: lookup
  17. Reichert C, Campe A, Walter S, Pfaender S, Welsch K, Ruddat I, Sieme H, Feige K, Steinmann E, Cavalleri JMV. Frequent occurrence of nonprimate hepacivirus infections in Thoroughbred breeding horses - A cross-sectional study for the occurrence of infections and potential risk factors.. Vet Microbiol 2017 May;203:315-322.
    doi: 10.1016/j.vetmic.2017.03.030pubmed: 28619163google scholar: lookup
  18. Scheel TK, Simmonds P, Kapoor A. Surveying the global virome: identification and characterization of HCV-related animal hepaciviruses.. Antiviral Res 2015 Mar;115:83-93.
    doi: 10.1016/j.antiviral.2014.12.014pmc: PMC5081135pubmed: 25545071google scholar: lookup
  19. Simmonds P. The origin of hepatitis C virus.. Curr Top Microbiol Immunol 2013;369:1-15.
    pubmed: 23463195doi: 10.1007/978-3-642-27340-7_1google scholar: lookup
  20. Simons JN, Leary TP, Dawson GJ, Pilot-Matias TJ, Muerhoff AS, Schlauder GG, Desai SM, Mushahwar IK. Isolation of novel virus-like sequences associated with human hepatitis.. Nat Med 1995 Jun;1(6):564-9.
    doi: 10.1038/nm0695-564pubmed: 7585124google scholar: lookup
  21. Tanaka T, Kasai H, Yamashita A, Okuyama-Dobashi K, Yasumoto J, Maekawa S, Enomoto N, Okamoto T, Matsuura Y, Morimatsu M, Manabe N, Ochiai K, Yamashita K, Moriishi K. Hallmarks of hepatitis C virus in equine hepacivirus.. J Virol 2014 Nov;88(22):13352-66.
    doi: 10.1128/JVI.02280-14pmc: PMC4249100pubmed: 25210167google scholar: lookup

Citations

This article has been cited 1 times.
  1. Pacchiarotti G, Nardini R, Scicluna MT. Equine Hepacivirus: A Systematic Review and a Meta-Analysis of Serological and Biomolecular Prevalence and a Phylogenetic Update.. Animals (Basel) 2022 Sep 20;12(19).
    doi: 10.3390/ani12192486pubmed: 36230228google scholar: lookup
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.