FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Braz J Microbiol / Seroepidemiology of Paracoccidioides brasiliensis infection ...
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2015; 46(2); 513-517; doi: 10.1590/S1517-838246246220140559

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil.

Abstract: Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.
Get Full Text
Publication Date: 2015-06-01 PubMed ID: 26273267PubMed Central: PMC4507544DOI: 10.1590/S1517-838246246220140559Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study aimed at understanding the rate of infection by Paracoccidioides brasiliensis, a harmful fungus, in horses located in the southwestern region of Rio Grande do Sul, Brazil. The horses, used as sentinels, revealed a 12% infection rate, implying the presence of the fungus in the examined region.

Research Purpose and Context

  • Paracoccidioides brasiliensis is a fungus and the cause of paracoccidioidomycosis, a major systemic mycosis (fungal infection) in Brazil. The infection is notably prevalent in the state of Rio Grande do Sul, which is considered endemic for the disease.
  • The main purpose of this study was to investigate the infection rate of P. brasiliensis in horses within the mesoregion of Southwest Riograndense. The horses were used as sentinel animals, enabling researchers to gain insights into the ecological presence of P. brasiliensis in the region.

Methodology

  • Two serological techniques, double immunodiffusion in agar gel (AGID) and indirect ELISA, were used to detect the presence of anti-gp43 P. brasiliensis antibody in horses.
  • The study focused on five distinct farms in the Bagé, RS, Brazil region. In total, 200 Pure Blood English horses aged up to two years were selected for the study. These animals were born and raised exclusively at the study farms, providing a controlled sample for the research.

Findings

  • The ELISA test results indicated a 12% infection rate, as the anti-gp43 antibodies were present in these horses.
  • The infection rates varied by farms, ranging from 0% to 30%. These variations in infection rates across different farms were statistically significant (p < 0.001).
  • However, the AGID method did not detect any positive cases among the serum samples collected from the horses.

Conclusion

  • The study concluded that there is a presence of P. brasiliensis infection amongst horses in the rural area of Rio Grande do Sul, Brazil. This indicates the overall presence and potential proliferation of this harmful fungus within the region.

Cite This Article

APA
Albano AP, Klafke GB, Brandolt TM, Da Hora VP, Nogueira CE, Xavier MO, Meireles MC. (2015). Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil. Braz J Microbiol, 46(2), 513-517. https://doi.org/10.1590/S1517-838246246220140559

Publication

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
NlmUniqueID: 101095924
Country: Brazil
Language: English
Volume: 46
Issue: 2
Pages: 513-517

Researcher Affiliations

Albano, Ana Paula Neuschrank
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Klafke, Gabriel Baracy
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Brandolt, Tchana Martinez
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Da Hora, Vanusa Pousada
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Nogueira, Carlos Eduardo Wayne
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Xavier, Melissa Orzechowski
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Meireles, Mário Carlos Araújo
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

MeSH Terms

  • Animals
  • Antibodies, Fungal / blood
  • Brazil / epidemiology
  • Enzyme-Linked Immunosorbent Assay
  • Horse Diseases / epidemiology
  • Horse Diseases / microbiology
  • Horses
  • Paracoccidioides / immunology
  • Paracoccidioidomycosis / epidemiology
  • Paracoccidioidomycosis / microbiology
  • Paracoccidioidomycosis / veterinary
  • Seroepidemiologic Studies

References

This article includes 23 references
  1. Albano AP, Klafke GB, Brandolt TM, Da Hora VP, Minello LF, Jorge S, Santos EO, Behling GM, Camargo ZP, Xavier MO, Meireles MC. Wild animals as sentinels of Paracoccidioides brasiliensis in the state of Rio Grande do Sul, Brazil.. Mycopathologia 2014 Apr;177(3-4):207-15.
    pubmed: 24570039doi: 10.1007/s11046-014-9731-ygoogle scholar: lookup
  2. Bagagli E, Bosco SMG. Armadillos and dimorphic pathogenic fungi: Ecological and evolutionary aspects. University Press of Florida; Gainesville: 2008; pp. 103–110.
  3. Bártholo RM, Florião RA, Oliveira LPA. Um caso fácil: um diagnóstico rápido. Bol Pneumol Sanit 2000;8:2.
  4. Brummer E, Castaneda E, Restrepo A. Paracoccidioidomycosis: an update.. Clin Microbiol Rev 1993 Apr;6(2):89-117.
    pmc: PMC358272pubmed: 8472249doi: 10.1128/cmr.6.2.89google scholar: lookup
  5. De Camargo Z, Unterkircher C, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests.. J Clin Microbiol 1988 Oct;26(10):2147-51.
    pmc: PMC266834pubmed: 3141460doi: 10.1128/jcm.26.10.2147-2151.1988google scholar: lookup
  6. Canteros CE, Madariaga MJ, Lee W, Rivas MC, Davel G, Iachini R. [Endemic fungal pathogens in a rural setting of Argentina: seroepidemiological study in dogs].. Rev Iberoam Micol 2010 Mar 31;27(1):14-9.
    pubmed: 20097588doi: 10.1016/j.riam.2009.11.002google scholar: lookup
  7. Conti-Díaz IA, Alvarez BJ, Gezuele E, González Marini H, Duarte J, Falcón J. [Intradermal reaction survey with paracoccidioidin and histoplasmin in horses].. Rev Inst Med Trop Sao Paulo 1972 Nov-Dec;14(6):372-6.
    pubmed: 4675643
  8. Conti Días IA. On the unknown ecological niche of Paracoccidioides brasiliensis: our hypothesis of 1989: present status and perspectives.. Rev Inst Med Trop Sao Paulo 2007 Mar-Apr;49(2):131-4.
    pubmed: 17505676doi: 10.1590/s0036-46652007000200014google scholar: lookup
  9. Corte AC, Gennari SM, Labruna MB. Paracoccidioides brasiliensis infection in dogs from Western Brazilian Amazon. Pesq Vet Bras 2012;32:649–652.
  10. Corte AC, Itano EM, Freire RL. Detection of antibodies to Paracoccidioides brasiliensis in horses from northern region of Parana State. Semina Ciênc Agr 2009;30:431–436.
  11. Corte AC, Svoboda WK, Navarro IT, Freire RL, Malanski LS, Shiozawa MM, Ludwig G, Aguiar LM, Passos FC, Maron A, Camargo ZP, Itano EN, Ono MA. Paracoccidioidomycosis in wild monkeys from Paraná State, Brazil.. Mycopathologia 2007 Nov;164(5):225-8.
    pubmed: 17914662doi: 10.1007/s11046-007-9059-ygoogle scholar: lookup
  12. Costa EO, Diniz LS, Netto CF. The prevalence of positive intradermal reactions to paracoccidioidin in domestic and wild animals in São Paulo, Brazil.. Vet Res Commun 1995;19(2):127-30.
    pubmed: 7645195doi: 10.1007/bf01839278google scholar: lookup
  13. FEE - Fundação de Economia e Estatística. Rio Grande do Sul,nBrasil 2013. [Acessed 16 Sep 2013]. Available at: http://www.fee.rs.gov.br/feedados/consulta/unidades_geo_mesos.asp?n=Mesorregi%E3o%20Sudeste%20Rio-Grandense.
  14. Marques AS. Paracoccidioidomicose: atualização epidemiológica, clínica e terapêutica. An Bras Dermatol 2003;78:2.
  15. Oliveira GG, Navarro IT, Freire RL, Belitardo DR, Silveira LH, Camargo ZP, Itano EN, Ono MA. Serological survey of Paracoccidioidomycosis in sheep.. Mycopathologia 2012 Jan;173(1):63-8.
    pubmed: 21850559doi: 10.1007/s11046-011-9463-1google scholar: lookup
  16. Ono MA, Bracarense AP, Morais HS, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: a seroepidemiologic study.. Med Mycol 2001 Jun;39(3):277-82.
    pubmed: 11446531doi: 10.1080/mmy.39.3.277.282google scholar: lookup
  17. Palmeiro M, Cherubini K, Yurgel LS. Paracoccidioidomicose – Revisão da Literatura. Sci Med 2005;15:4.
  18. Puccia R, Travassos LR. 43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease.. J Clin Microbiol 1991 Aug;29(8):1610-5.
    pmc: PMC270171pubmed: 1722220doi: 10.1128/jcm.29.8.1610-1615.1991google scholar: lookup
  19. Ramos-E-Silva M, Saraiva Ldo E. Paracoccidioidomycosis.. Dermatol Clin 2008 Apr;26(2):257-69, vii.
    pubmed: 18346557doi: 10.1016/j.det.2007.11.005google scholar: lookup
  20. Restrepo A, McEwen JG, Castañeda E. The habitat of Paracoccidioides brasiliensis: how far from solving the riddle?. Med Mycol 2001 Jun;39(3):233-41.
    pubmed: 11446526doi: 10.1080/mmy.39.3.233.241google scholar: lookup
  21. Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis.. Mycopathologia 2006 Nov;162(5):325-9.
    pmc: PMC2780595pubmed: 17123030doi: 10.1007/s11046-006-0046-5google scholar: lookup
  22. Silveira LH, Paes RC, Medeiros EV, Itano EN, Camargo ZP, Ono MA. Occurrence of Antibodies to Paracoccidioides brasiliensis in dairy cattle from Mato Grosso do Sul, Brazil.. Mycopathologia 2008 Jun;165(6):367-71.
    pubmed: 18320347doi: 10.1007/s11046-008-9095-2google scholar: lookup
  23. Terçarioli GR, Bagagli E, Reis GM, Theodoro RC, Bosco Sde M, Macoris SA, Richini-Pereira VB. Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection.. BMC Microbiol 2007 Oct 22;7:92.
    pmc: PMC2180180pubmed: 17953742doi: 10.1186/1471-2180-7-92google scholar: lookup

Citations

This article has been cited 7 times.
  1. Hahn RC, Hagen F, Mendes RP, Burger E, Nery AF, Siqueira NP, Guevara A, Rodrigues AM, de Camargo ZP. Paracoccidioidomycosis: Current Status and Future Trends.. Clin Microbiol Rev 2022 Dec 21;35(4):e0023321.
    doi: 10.1128/cmr.00233-21pubmed: 36074014google scholar: lookup
  2. Brito TGN, Taborda M, Provenci B, Costa AN, Benard G. A Case-Control Study of Paracoccidioidomycosis in Women: The Hormonal Protection Revisited.. J Fungi (Basel) 2021 Aug 13;7(8).
    doi: 10.3390/jof7080655pubmed: 34436194google scholar: lookup
  3. Ashraf N, Kubat RC, Poplin V, Adenis AA, Denning DW, Wright L, McCotter O, Schwartz IS, Jackson BR, Chiller T, Bahr NC. Re-drawing the Maps for Endemic Mycoses.. Mycopathologia 2020 Oct;185(5):843-865.
    doi: 10.1007/s11046-020-00431-2pubmed: 32040709google scholar: lookup
  4. Mendes JF, Von Groll A, Poester VR, Brasil CL, Brandolt TM, Klafke GB, Nogueira CEW, Meireles MCA, Xavier MO. Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil.. Curr Microbiol 2019 Feb;76(2):258-262.
    doi: 10.1007/s00284-018-1621-ypubmed: 30600363google scholar: lookup
  5. Martinez R. New Trends in Paracoccidioidomycosis Epidemiology.. J Fungi (Basel) 2017 Jan 3;3(1).
    doi: 10.3390/jof3010001pubmed: 29371520google scholar: lookup
  6. Headley SA, Pretto-Giordano LG, Di Santis GW, Gomes LA, Macagnan R, da Nóbrega DF, Leite KM, de Alcântara BK, Itano EN, Alfieri AA, Ono MA. Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog.. Mycopathologia 2017 Apr;182(3-4):425-434.
    doi: 10.1007/s11046-016-0075-7pubmed: 27757778google scholar: lookup
  7. Teles AJ, Klafke GB, Cabana ÂL, Albano AP, Xavier MO, Meireles MC. Serological Investigation into Paracoccidioides brasiliensis Infection in Dogs from Southern Rio Grande do Sul, Brazil.. Mycopathologia 2016 Apr;181(3-4):323-8.
    doi: 10.1007/s11046-015-9972-4pubmed: 26608381google scholar: lookup
Subscribe to our newsletter
Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.