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Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2015; 46(2); 513-517; doi: 10.1590/S1517-838246246220140559

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil.

Abstract: Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and raised exclusively at the farms. Of these horses, 12% had anti-gp43 antibodies according to the ELISA results, with rates ranging from 0 to 30% according to the farm of origin (p < 0.001). Based on the immunodiffusion results, all equine serum samples were negative. These results indicate the presence of the fungus P. brasiliensis in the middle region of the southwestern state of Rio Grande do Sul, Brazil.
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Publication Date: 2015-06-01 PubMed ID: 26273267PubMed Central: PMC4507544DOI: 10.1590/S1517-838246246220140559Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study aimed at understanding the rate of infection by Paracoccidioides brasiliensis, a harmful fungus, in horses located in the southwestern region of Rio Grande do Sul, Brazil. The horses, used as sentinels, revealed a 12% infection rate, implying the presence of the fungus in the examined region.

Research Purpose and Context

  • Paracoccidioides brasiliensis is a fungus and the cause of paracoccidioidomycosis, a major systemic mycosis (fungal infection) in Brazil. The infection is notably prevalent in the state of Rio Grande do Sul, which is considered endemic for the disease.
  • The main purpose of this study was to investigate the infection rate of P. brasiliensis in horses within the mesoregion of Southwest Riograndense. The horses were used as sentinel animals, enabling researchers to gain insights into the ecological presence of P. brasiliensis in the region.

Methodology

  • Two serological techniques, double immunodiffusion in agar gel (AGID) and indirect ELISA, were used to detect the presence of anti-gp43 P. brasiliensis antibody in horses.
  • The study focused on five distinct farms in the Bagé, RS, Brazil region. In total, 200 Pure Blood English horses aged up to two years were selected for the study. These animals were born and raised exclusively at the study farms, providing a controlled sample for the research.

Findings

  • The ELISA test results indicated a 12% infection rate, as the anti-gp43 antibodies were present in these horses.
  • The infection rates varied by farms, ranging from 0% to 30%. These variations in infection rates across different farms were statistically significant (p < 0.001).
  • However, the AGID method did not detect any positive cases among the serum samples collected from the horses.

Conclusion

  • The study concluded that there is a presence of P. brasiliensis infection amongst horses in the rural area of Rio Grande do Sul, Brazil. This indicates the overall presence and potential proliferation of this harmful fungus within the region.

Cite This Article

APA
Albano AP, Klafke GB, Brandolt TM, Da Hora VP, Nogueira CE, Xavier MO, Meireles MC. (2015). Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil. Braz J Microbiol, 46(2), 513-517. https://doi.org/10.1590/S1517-838246246220140559

Publication

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
NlmUniqueID: 101095924
Country: Brazil
Language: English
Volume: 46
Issue: 2
Pages: 513-517

Researcher Affiliations

Albano, Ana Paula Neuschrank
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Klafke, Gabriel Baracy
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Brandolt, Tchana Martinez
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Da Hora, Vanusa Pousada
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Nogueira, Carlos Eduardo Wayne
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
Xavier, Melissa Orzechowski
  • Universidade Federal do Rio Grande, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil, Laboratório de Micologia, Faculdade de Medicina, Universidade Federal do Rio Grande, Rio Grande, RS, Brazil.
Meireles, Mário Carlos Araújo
  • Universidade Federal de Pelotas, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil, Departamento de Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

MeSH Terms

  • Animals
  • Antibodies, Fungal / blood
  • Brazil / epidemiology
  • Enzyme-Linked Immunosorbent Assay
  • Horse Diseases / epidemiology
  • Horse Diseases / microbiology
  • Horses
  • Paracoccidioides / immunology
  • Paracoccidioidomycosis / epidemiology
  • Paracoccidioidomycosis / microbiology
  • Paracoccidioidomycosis / veterinary
  • Seroepidemiologic Studies

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Citations

This article has been cited 8 times.
  1. Hahn RC, Hagen F, Mendes RP, Burger E, Nery AF, Siqueira NP, Guevara A, Rodrigues AM, de Camargo ZP. Paracoccidioidomycosis: Current Status and Future Trends. Clin Microbiol Rev 2022 Dec 21;35(4):e0023321.
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  2. Brito TGN, Taborda M, Provenci B, Costa AN, Benard G. A Case-Control Study of Paracoccidioidomycosis in Women: The Hormonal Protection Revisited. J Fungi (Basel) 2021 Aug 13;7(8).
    doi: 10.3390/jof7080655pubmed: 34436194google scholar: lookup
  3. Ashraf N, Kubat RC, Poplin V, Adenis AA, Denning DW, Wright L, McCotter O, Schwartz IS, Jackson BR, Chiller T, Bahr NC. Re-drawing the Maps for Endemic Mycoses. Mycopathologia 2020 Oct;185(5):843-865.
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  4. Mendes JF, Von Groll A, Poester VR, Brasil CL, Brandolt TM, Klafke GB, Nogueira CEW, Meireles MCA, Xavier MO. Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil. Curr Microbiol 2019 Feb;76(2):258-262.
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  6. Headley SA, Pretto-Giordano LG, Di Santis GW, Gomes LA, Macagnan R, da Nóbrega DF, Leite KM, de Alcântara BK, Itano EN, Alfieri AA, Ono MA. Paracoccidioides brasiliensis-associated dermatitis and lymphadenitis in a dog. Mycopathologia 2017 Apr;182(3-4):425-434.
    doi: 10.1007/s11046-016-0075-7pubmed: 27757778google scholar: lookup
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    doi: 10.3390/jof10030165pubmed: 38535174google scholar: lookup
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