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Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum.
Abstract: In the context of a serosurvey conducted on the Anaplasma marginale prevalence in Swiss cattle, we suspected that a serological cross-reactivity between A. marginale and A. phagocytophilum might exist. In the present study we demonstrate that cattle, sheep and horses experimentally infected with A. phagocytophilum not only develop antibodies to A. phagocytophilum (detected by immunofluorescent-antibody assay) but also to A. marginale (detected by a competitive enzyme-linked immunosorbent assay). Conversely, calves experimentally infected with A. marginale also developed antibodies to A. phagocytophilum using the same serological tests. The identity of 63% determined in silico within a 209-amino-acid sequence of major surface protein 5 of an isolate of A. marginale and one of A. phagocytophilum supported the observed immunological cross-reactivity. These observations have important consequences for the serotesting of both, A. marginale and A. phagocytophilum infection of several animal species. In view of these new findings, tests that have been considered specific for either infection must be interpreted carefully.
Publication Date: 2005-10-08 PubMed ID: 16210480PubMed Central: PMC1247822DOI: 10.1128/CDLI.12.10.1177-1183.2005Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Amino Acid Sequence
- Animal Species
- Antibodies
- Comparative Study
- Diagnosis
- Disease
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Health
- Horses
- Immunofluorescence Assay
- Immunology
- Infection
- Infectious Disease
- Serodiagnosis
- Serological Surveys
- Seroprevalence
- Tick-Borne Diseases
- Veterinary Medicine
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article investigates the potential for a serological cross-reaction between two strains of Anaplasma bacteria, A. marginale and A. phagocytophilum, in cattle, sheep, and horses. The study found that this cross-reactivity affects the accuracy of existing diagnostic tests for these infections.
Background of the Study
- Researchers commenced this study due to a suspected serological cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum during a serosurvey being conducted on Swiss cattle.
- Anaplasma marginale and Anaplasma phagocytophilum are bacteria known to cause diseases in various animal species. Serological cross-reactivity refers to a situation where antibodies raised against one organism also react with another different yet related organism.
Methodology
- Experimentally, the researchers infected cattle, sheep, and horses with A. phagocytophilum to observe the development of antibodies not only to A. phagocytophilum but also to A. marginale.
- On the other hand, calves infected with A. marginale were observed to develop antibodies to A. phagocytophilum.
- The detection of these antibodies was performed using a combination of serological tests: immunofluorescent-antibody assay for A. phagocytophilum and competitive enzyme-linked immunosorbent assay for A. marginale.
Results and Significance
- A significant finding was the discovery of the identity of 63% determined in silico within a 209-amino-acid sequence of major surface protein 5 of an isolate of A. marginale and one of A. phagocytophilum which supported the observed immunological cross-reactivity.
- This cross-reactivity has significant implications for the testing of both A. marginale and A. phagocytophilum infection in various animal species.
- Results suggest that tests previously considered specific for detecting either of these infections may not be as accurate as thought, and therefore must be interpreted with caution.
Cite This Article
APA
Dreher UM, de la Fuente J, Hofmann-Lehmann R, Meli ML, Pusterla N, Kocan KM, Woldehiwet Z, Braun U, Regula G, Staerk KD, Lutz H.
(2005).
Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum.
Clin Diagn Lab Immunol, 12(10), 1177-1183.
https://doi.org/10.1128/CDLI.12.10.1177-1183.2005 Publication
Researcher Affiliations
- Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland.
MeSH Terms
- Anaplasma marginale / immunology
- Anaplasma phagocytophilum / immunology
- Anaplasmosis / diagnosis
- Animals
- Antibodies, Bacterial / blood
- Antibodies, Bacterial / immunology
- Cattle
- Cross Reactions / immunology
- Enzyme-Linked Immunosorbent Assay
- Horses
- Sensitivity and Specificity
- Serologic Tests / standards
- Sheep
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