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Home / Equine Research Bank / J Clin Microbiol / Serologic evidence of canine and equine ehrlichiosis in nort...
Journal of clinical microbiology1993; 31(11); 2857-2860; doi: 10.1128/jcm.31.11.2857-2860.1993

Serologic evidence of canine and equine ehrlichiosis in northeastern United States.

Abstract: In a retrospective study, indirect fluorescent-antibody staining methods were used to detect immunoglobulins to Ehrlichia canis and Ehrlichia risticii in canine and equine sera that had originally been analyzed for antibodies to Borrelia burgdorferi. Analyses of 60 dog serum specimens collected in Connecticut and New York State during 1986 revealed antibodies to E. canis in 7 (11.7%) specimens; titration endpoints ranged from 1:40 to 1:320. Three of these dogs had anemia. Of the 187 equine serum specimens obtained in Connecticut during 1985 and analyzed by indirect fluorescent-antibody staining methods, 17 (9.1%) contained antibodies to E. risticii. Maximal antibody titers of 1:1,280 were recorded for serum specimens collected from three equids during May and July. We conclude that canine and equine ehrlichiosis coexist with Lyme borreliosis in Connecticut and the lower Hudson River Valley of New York State.
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Publication Date: 1993-11-01 PubMed ID: 8263167PubMed Central: PMC266144DOI: 10.1128/jcm.31.11.2857-2860.1993Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research presents findings from a retrospective study that provides evidence for the presence of ehrlichiosis, a tick-borne disease, in dogs and horses in Connecticut and the lower Hudson River Valley of New York State.

Objectives and Methodology of the Study

  • The main objective of this study was to detect the presence of antibodies for Ehrlichia canis and Ehrlichia risticii, both of which cause ehrlichiosis, in canine and equine sera. Ehrlichiosis is a tick-borne disease that affects various animals and humans.
  • This research employed a retrospective study design and the antibodies were detected using indirect fluorescent-antibody staining methods.
  • The samples used for the study were canine and equine sera that had originally been analyzed for antibodies to Borrelia burgdorferi, the bacteria that causes Lyme disease.
  • A total of 60 dog serum samples and 187 equine serum samples were analyzed for this study.

Key Findings of the Study

  • Analyzing the 60 dog serum specimens collected in Connecticut and New York State in 1986, researchers found antibodies to E. canis in 7 specimens. This amounted to an occurrence rate of about 11.7%.
  • The detected antibody titration endpoints, which essentially means the highest dilution point of a solution at which antibodies are still able to bind to antigens and yield a positive reaction, ranged from 1:40 to 1:320.
  • Three of the dogs showing antibodies to E. canis also had anemia, a condition often associated with ehrlichiosis.
  • Of the 187 equine serum specimens, 17 (or 9.1%) contained antibodies to E. risticii, another species of ehrlichia bacteria. The maximal antibody titers recorded for these samples were 1:1,280.

Conclusions of the Study

  • The study concluded that both canine and equine ehrlichiosis are present in the Connecticut region and the lower Hudson River Valley of New York State.
  • The findings suggest the existence of ehrlichiosis in these regions alongside Lyme borreliosis, another tick-borne disease.
  • This study provides a crucial foundation for more extensive research on the prevalence and impacts of tick-borne diseases in animals in the northeastern United States.

Cite This Article

APA
Magnarelli LA, Anderson JF. (1993). Serologic evidence of canine and equine ehrlichiosis in northeastern United States. J Clin Microbiol, 31(11), 2857-2860. https://doi.org/10.1128/jcm.31.11.2857-2860.1993

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 31
Issue: 11
Pages: 2857-2860

Researcher Affiliations

Magnarelli, L A
  • Department of Entomology, Connecticut Agricultural Experiment Station, New Haven 06504.
Anderson, J F

    MeSH Terms

    • Animals
    • Antibodies, Bacterial / blood
    • Dog Diseases / diagnosis
    • Dogs
    • Ehrlichia / immunology
    • Ehrlichiosis / diagnosis
    • Ehrlichiosis / veterinary
    • Female
    • Fluorescent Antibody Technique
    • Horse Diseases / diagnosis
    • Horses
    • Male
    • Retrospective Studies

    References

    This article includes 23 references
    1. Ristic M, Huxsoll DL, Weisiger RM, Hildebrandt PK, Nyindo MB. Serological diagnosis of tropical canine pancytopenia by indirect immunofluorescence.. Infect Immun 1972 Sep;6(3):226-31.
      pubmed: 4118045doi: 10.1128/iai.6.3.226-231.1972google scholar: lookup
    2. Oliver JH Jr, Owsley MR, Hutcheson HJ, James AM, Chen C, Irby WS, Dotson EM, McLain DK. Conspecificity of the ticks Ixodes scapularis and I. dammini (Acari: Ixodidae).. J Med Entomol 1993 Jan;30(1):54-63.
      pubmed: 8433346doi: 10.1093/jmedent/30.1.54google scholar: lookup
    3. Anderson JF, Magnarelli LA, Kurz J. Intraerythrocytic parasites in rodent populations of Connecticut: Babesia and Grahamella species.. J Parasitol 1979 Aug;65(4):599-604.
      pubmed: 512755
    4. Magnarelli LA, Anderson JF, Philip RN, Burgdorfer W. Antibodies to spotted fever-group rickettsiae in dogs and prevalence of infected ticks in southern Connecticut.. Am J Vet Res 1982 Apr;43(4):656-9.
      pubmed: 6803626
    5. Keefe TJ, Holland CJ, Salyer PE, Ristic M. Distribution of Ehrlichia canis among military working dogs in the world and selected civilian dogs in the United States.. J Am Vet Med Assoc 1982 Aug 1;181(3):236-8.
      pubmed: 7050062
    6. Magnarelli LA, Anderson JF, Burgdorfer W, Chappell WA. Parasitism by Ixodes dammini (Acari: Ixodidae) and antibodies to spirochetes in mammals at Lyme disease foci in Connecticut, USA.. J Med Entomol 1984 Jan 26;21(1):52-7.
      pubmed: 6694176doi: 10.1093/jmedent/21.1.52google scholar: lookup
    7. Magnarelli LA, Anderson JF, Burgdorfer W, Philip RN, Chappell WA. Antibodies to Rickettsia rickettsii in Peromyscus leucopus from a focus of Rocky Mountain spotted fever in Connecticut.. Can J Microbiol 1984 Apr;30(4):491-4.
      pubmed: 6744122doi: 10.1139/m84-072google scholar: lookup
    8. Holland CJ, Ristic M, Cole AI, Johnson P, Baker G, Goetz T. Isolation, experimental transmission, and characterization of causative agent of Potomac horse fever.. Science 1985 Feb 1;227(4686):522-4.
      pubmed: 3880925doi: 10.1126/science.3880925google scholar: lookup
    9. Ristic M, Holland CJ, Dawson JE, Sessions J, Palmer J. Diagnosis of equine monocytic ehrlichiosis (Potomac horse fever) by indirect immunofluorescence.. J Am Vet Med Assoc 1986 Jul 1;189(1):39-46.
      pubmed: 3525479
    10. Palmer JE, Whitlock RH, Benson CE. Equine ehrlichial colitis (Potomac horse fever): recognition of the disease in Pennsylvania, New Jersey, New York, Ohio, Idaho, and Connecticut.. J Am Vet Med Assoc 1986 Jul 15;189(2):197-9.
      pubmed: 3744978
    11. Magnarelli LA, Anderson JF, Schreier AB, Ficke CM. Clinical and serologic studies of canine borreliosis.. J Am Vet Med Assoc 1987 Nov 1;191(9):1089-94.
      pubmed: 3319989
    12. Magnarelli LA, Anderson JF, Shaw E, Post JE, Palka FC. Borreliosis in equids in northeastern United States.. Am J Vet Res 1988 Mar;49(3):359-62.
      pubmed: 3282461
    13. Hoskins JD, Breitschwerdt EB, Gaunt SD, French TW, Burgdorfer W. Antibodies to Ehrlichia canis, Ehrlichia platys, and spotted fever group rickettsiae in Louisiana dogs.. J Vet Intern Med 1988 Apr-Jun;2(2):55-9.
      pubmed: 3146636doi: 10.1111/j.1939-1676.1988.tb02793.xgoogle scholar: lookup
    14. Magnarelli LA, Anderson JF. Class-specific and polyvalent enzyme-linked immunosorbent assays for detection of antibodies to Borrelia burgdorferi in equids.. J Am Vet Med Assoc 1989 Nov 15;195(10):1365-8.
      pubmed: 2684937
    15. Magnarelli LA, Litwin HJ, Holland CJ, Anderson JF, Ristic M. Canine ehrlichiosis in Connecticut.. J Clin Microbiol 1990 Feb;28(2):366-7.
      pubmed: 2312682doi: 10.1128/jcm.28.2.366-367.1990google scholar: lookup
    16. McDade JE. Ehrlichiosis--a disease of animals and humans.. J Infect Dis 1990 Apr;161(4):609-17.
      pubmed: 2181027doi: 10.1093/infdis/161.4.609google scholar: lookup
    17. Magnarelli LA, Anderson JF, Schreier AB. Persistence of antibodies to Borrelia burgdorferi in dogs of New York and Connecticut.. J Am Vet Med Assoc 1990 Apr 1;196(7):1064-8.
      pubmed: 2329074
    18. Anziani OS, Ewing SA, Barker RW. Experimental transmission of a granulocytic form of the tribe Ehrlichieae by Dermacentor variabilis and Amblyomma americanum to dogs.. Am J Vet Res 1990 Jun;51(6):929-31.
      pubmed: 2368951
    19. Magnarelli LA, Anderson JF, Levine HR, Levy SA. Tick parasitism and antibodies to Borrelia burgdorferi in cats.. J Am Vet Med Assoc 1990 Jul 1;197(1):63-6.
      pubmed: 2196252
    20. Woody BJ, Hoskins JD. Ehrlichial diseases of dogs.. Vet Clin North Am Small Anim Pract 1991 Jan;21(1):75-98.
      pubmed: 2014630doi: 10.1016/s0195-5616(91)50009-7google scholar: lookup
    21. Rikihisa Y. The tribe Ehrlichieae and ehrlichial diseases.. Clin Microbiol Rev 1991 Jul;4(3):286-308.
      pubmed: 1889044doi: 10.1128/CMR.4.3.286google scholar: lookup
    22. Magnarelli LA, Andreadis TG, Stafford KC 3rd, Holland CJ. Rickettsiae and Borrelia burgdorferi in ixodid ticks.. J Clin Microbiol 1991 Dec;29(12):2798-804.
      pubmed: 1757551doi: 10.1128/jcm.29.12.2798-2804.1991google scholar: lookup
    23. Groves MG, Dennis GL, Amyx HL, Huxsoll DL. Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus).. Am J Vet Res 1975 Jul;36(7):937-40.
      pubmed: 1147359

    Citations

    This article has been cited 4 times.
    1. Luo L, Sun J, Yan J, Wang C, Zhang Z, Zhao L, Han H, Tong Z, Liu M, Wu Y, Wen H, Zhang R, Xue Z, Sun X, Li K, Ma D, Liu J, Huang Y, Ye L, Li W, Jiang J, Yu XJ. Detection of a Novel Ehrlichia Species in Haemaphysalis longicornis Tick from China. Vector Borne Zoonotic Dis 2016 Jun;16(6):363-7.
      doi: 10.1089/vbz.2015.1898pubmed: 27135624google scholar: lookup
    2. Stich RW, Schaefer JJ, Bremer WG, Needham GR, Jittapalapong S. Host surveys, ixodid tick biology and transmission scenarios as related to the tick-borne pathogen, Ehrlichia canis. Vet Parasitol 2008 Dec 20;158(4):256-73.
      doi: 10.1016/j.vetpar.2008.09.013pubmed: 18963493google scholar: lookup
    3. Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. J Clin Microbiol 1997 Jan;35(1):169-73.
      doi: 10.1128/jcm.35.1.169-173.1997pubmed: 8968901google scholar: lookup
    4. Magnarelli LA, Stafford KC 3rd, Mather TN, Yeh MT, Horn KD, Dumler JS. Hemocytic rickettsia-like organisms in ticks: serologic reactivity with antisera to Ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR. J Clin Microbiol 1995 Oct;33(10):2710-4.
      doi: 10.1128/jcm.33.10.2710-2714.1995pubmed: 8567911google scholar: lookup
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