Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes.
Abstract: A series of viral reassortants was prepared between equine rotaviruses H1 (G5), H2 (G3), and L338 (G13) and human rotavirus ST3 (G4). All contained the VP4 cognate gene segment 4 from the equine parental virus and the VP7 cognate gene segment 9 from ST3. Using these viruses and antisera prepared to them, it was shown that each of the three equine viruses possessed a serologically distinct VP4 or P serotype with a > or = 16-fold difference in reciprocal cross-neutralization titers. H1 VP4 was closely related to that of porcine virus OSU, i.e., P7. L338 gene 4 was sequenced, and the sequence and serological data indicated that it constituted a novel P serotype L338. P serotype H2 was predominant among our cell culture-adapted equine rotavirus strains, but showed some serological cross-reactivity.
Publication Date: 1994-06-01 PubMed ID: 7514321DOI: 10.1006/viro.1994.1302Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article presents studies on equine rotaviruses, showing that each of the three strains tested has a serologically distinct VP4 protein, leading to the identification of three different P serotypes.
Objective of the Research
- The researchers aimed to investigate the serological and genomic characteristics of VP4 proteins in equine rotaviruses. Their goal was to understand the different types or serotypes of these viruses, specifically focusing on the so-called P serotypes.
Methodology
- The researchers prepared a series of viral reassortants (hybrid viruses) between different equine rotavirus strains (H1, H2, L338) and a human rotavirus strain (ST3).
- These reassortants contained specific gene segments from both equine and human strains: the VP4 gene from equine rotavirus and the VP7 gene from the human rotavirus strain ST3.
- The researchers then used these created viruses along with other prepared antisera to investigate the serological characteristics of the VP4 protein in equine rotaviruses.
Findings
- They found that each equine rotavirus possessed a distinct VP4 or P serotype, as demonstrated by a 16-fold difference in cross-neutralization titers, a measure of a virus’ ability to neutralize an immune response.
- Further more, they found that the VP4 protein of rotavirus strain H1 is closely related to that of the porcine virus OSU, identified as P7.
- Scientists sequenced gene 4 from the strain L338 and combined with the serological data, it pointed to the fact that it was a novel P serotype labelled L338.
- The H2 P serotype was found to be the most common among the scientists’ collection of equine rotaviruses adapted for cell culture. However, it also exhibited some serological cross-reactivity, which means that antibodies against this serotype may also react with other serotypes.
Significance
- The research provides a deeper understanding of the different P serotypes of equine rotaviruses, which can contribute to the development of more effective treatments and prevention strategies for diseases caused by these viruses in horses.
Cite This Article
APA
Isa P, Snodgrass DR.
(1994).
Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes.
Virology, 201(2), 364-372.
https://doi.org/10.1006/viro.1994.1302 Publication
Researcher Affiliations
- Moredun Research Institute, Edinburgh, United Kingdom.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Capsid / classification
- Capsid / genetics
- Capsid / immunology
- Capsid Proteins
- DNA, Viral
- Epitopes / immunology
- Genes, Viral
- Humans
- Molecular Sequence Data
- Neutralization Tests
- Rotavirus / classification
- Rotavirus / genetics
- Rotavirus / immunology
- Rotavirus Infections / microbiology
- Sequence Homology, Amino Acid
- Serotyping
Citations
This article has been cited 12 times.- Pathak A, Gulati BR, Maan S, Mor S, Kumar D, Soman R, Punia S, Chaudhary D, Khurana SK. Complete Genome Sequencing Reveals Unusual Equine Rotavirus A of Bat Origin from India.. J Virol 2022 Oct 26;96(20):e0140822.
- Uprety T, Sreenivasan CC, Hause BM, Li G, Odemuyiwa SO, Locke S, Morgan J, Zeng L, Gilsenan WF, Slovis N, Metcalfe L, Carter CN, Timoney P, Horohov D, Wang D, Erol E, Adam E, Li F. Identification of a Ruminant Origin Group B Rotavirus Associated with Diarrhea Outbreaks in Foals.. Viruses 2021 Jul 9;13(7).
- Bailey KE, Gilkerson JR, Browning GF. Equine rotaviruses--current understanding and continuing challenges.. Vet Microbiol 2013 Nov 29;167(1-2):135-44.
- Collins PJ, Cullinane A, Martella V, O'Shea H. Molecular characterization of equine rotavirus in Ireland.. J Clin Microbiol 2008 Oct;46(10):3346-54.
- Ciarlet M, I a P, Conner ME, Liprandi F. Antigenic and molecular analyses reveal that the equine rotavirus strain H-1 is closely related to porcine, but not equine, rotaviruses: interspecies transmission from pigs to horses?. Virus Genes 2001 Jan;22(1):5-20.
- Padilla-Noriega L, Méndez-Toss M, Menchaca G, Contreras JF, Romero-Guido P, Puerto FI, Guiscafré H, Mota F, Herrera I, Cedillo R, Muñoz O, Calva J, Guerrero ML, Coulson BS, Greenberg HB, López S, Arias CF. Antigenic and genomic diversity of human rotavirus VP4 in two consecutive epidemic seasons in Mexico.. J Clin Microbiol 1998 Jun;36(6):1688-92.
- Alfieri AA, Leite JP, Nakagomi O, Kaga E, Woods PA, Glass RI, Gentsch JR. Characterization of human rotavirus genotype P[8]G5 from Brazil by probe-hybridization and sequence.. Arch Virol 1996;141(12):2353-64.
- Ciarlet M, Estes MK, Barone C, Ramig RF, Conner ME. Analysis of host range restriction determinants in the rabbit model: comparison of homologous and heterologous rotavirus infections.. J Virol 1998 Mar;72(3):2341-51.
- Isa P, Wood AR, Netherwood T, Ciarlet M, Imagawa H, Snodgrass DR. Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes.. Arch Virol 1996;141(9):1601-12.
- Browning GF, Begg AP. Prevalence of G and P serotypes among equine rotaviruses in the faeces of diarrhoeic foals.. Arch Virol 1996;141(6):1077-89.
- Mahajan NP, Rao CD. Nucleotide sequence and expression in E. coli of the complete P4 type VP4 from a G2 serotype human rotavirus.. Arch Virol 1996;141(2):315-29.
- Li B, Hoshino Y, Gorziglia M. Identification of a unique VP4 serotype that is shared by a human rotavirus (69M strain) and an equine rotavirus (H-2 strain).. Arch Virol 1996;141(1):155-60.
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