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Home / Equine Research Bank / J Gen Virol / Serological and genomic characterization of L338, a novel eq...
The Journal of general virology1991; 72 ( Pt 5); 1059-1064; doi: 10.1099/0022-1317-72-5-1059

Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype.

Abstract: A group A rotavirus designated L338 was isolated from the faeces of a diarrhoeic foal and was compared to 11 standard G serotype strains of group A rotaviruses by cross-neutralization. It was clearly distinct from serotypes G1 to G11 and thus representative of a novel rotavirus G serotype tentatively designated G13. The nucleic acid sequence of the virion protein 7 (VP7) coding region was determined and the deduced amino acid sequence compared to published sequences. Within VP7 regions A and B, L338 was clearly distinct from serotypes G1 to G12 (excluding G7 which has not been sequenced), but region C was very similar to those of G3 and G8. This further questions the significance of region C in determining serotype specificity of at least three distinct rotavirus G serotypes.
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Publication Date: 1991-05-01 PubMed ID: 1851806DOI: 10.1099/0022-1317-72-5-1059Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article emphasizes the discovery and analysis of a new strain of equine group A rotavirus, tentatively labelled as G13, extracted from a foal’s feces suffering from diarrhea. The authors examine and compare its genetic and serological properties with other known G serotype strains of group A rotaviruses.

Detection and Characterization of L338

  • The research team isolated a group A rotavirus, L338, from the feces of a sick foal displaying diarrhea.
  • Using cross-neutralization methods, they compared this isolated sample to the 11 standard G serotype strains of group A rotaviruses to confirm its distinctiveness.
  • After comparison, it was established that the L338 strain was unique and differed from serotypes G1 to G11. Therefore, the researchers proposed the strain as a novel G serotype, tentatively named G13.

Genomic Examination of L338

  • The scientists decoded the nucleic acid sequence specific to the virion protein 7 (VP7) coding region of the L338.
  • They compared the inferred amino acid sequence with already published sequences.
  • The comparison showed that L338 was distinct from serotypes G1 to G12 in regions A and B of VP7. Serotype G7 was excluded in this comparison as its sequence is not yet available.

Significance of Region C

  • The VP7 region C of L338 displayed significant similarity to the corresponding regions of G3 and G8 serotypes.
  • This observation led to speculation about the role of region C in determining rotavirus G serotype specificity.
  • The research identifies the need for further investigation to understand the potential role of region C, as it appears to be consistent across diverse rotavirus group A G serotypes.

In conclusion, the study contributes valuable new knowledge about rotaviruses by identifying a distinct equine Group A strain, tentatively named G13. The investigation of its genetic profile provides insights into rotavirus genomic structure and serotype specificity, paving the way for further research in this area.

Cite This Article

APA
Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR. (1991). Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol, 72 ( Pt 5), 1059-1064. https://doi.org/10.1099/0022-1317-72-5-1059

Publication

The Journal of general virology
ISSN: 0022-1317
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 72 ( Pt 5)
Pages: 1059-1064

Researcher Affiliations

Browning, G F
  • Moredun Research Institute, Edinburgh, U.K.
Chalmers, R M
    Fitzgerald, T A
      Snodgrass, D R

        MeSH Terms

        • Amino Acid Sequence
        • Animals
        • Antigens, Viral
        • Base Sequence
        • Capsid / genetics
        • Capsid Proteins
        • Cross Reactions
        • Electrophoresis, Polyacrylamide Gel
        • Genes, Viral
        • Horses
        • Immune Sera
        • Molecular Sequence Data
        • Neutralization Tests
        • Rotavirus / classification
        • Rotavirus / genetics
        • Rotavirus / isolation & purification
        • Sequence Homology, Nucleic Acid
        • Serotyping

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