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Home / Equine Research Bank / Korean J Parasitol / Serological Detection of Borrelia burgdorferi among Horses i...
The Korean journal of parasitology2016; 54(1); 97-101; doi: 10.3347/kjp.2016.54.1.97

Serological Detection of Borrelia burgdorferi among Horses in Korea.

Abstract: Lyme disease is a tick-borne zoonotic infectious disease caused by Borrelia burgdorferi. The present study assessed the infection status of B. burgdorferi among horses reared in Korea using ELISA and PCR. Between 2009 and 2013, blood samples were collected from 727 horses throughout Korea. Data for each animal including age, gender, breed, and region of sample collection were used for epidemiological analysis. Overall, 38 (5.2%; true prevalence: 5.5%) of 727 horses were seropositive by ELISA. There were statistically significant differences according to breed and region (P<0.001) whose differences might be attributed to the ecology of vector ticks and climate conditions. Using 2 nested PCR, none of the samples tested positive for B. burgdorferi. Thus, a positive ELISA result can indicate only that the tested horse was previously exposed to B. burgdorferi, with no certainty over the time of exposure. Since global warming is likely to increase the abundance of ticks in Korea, continuous monitoring of tick-borne diseases in Korean horses is needed.
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Publication Date: 2016-02-26 PubMed ID: 26951987PubMed Central: PMC4792323DOI: 10.3347/kjp.2016.54.1.97Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examines the presence of Lyme disease, caused by the Borrelia burgdorferi bacteria, in horses in Korea. The research indicates that while there were instances where horses were exposed to the bacteria, none of the tested samples were positively identified as having the disease.

Background and Methodology

  • The study focused on Lyme disease, a zoonotic infectious disease that is transferred to humans by ticks. This disease is caused by the bacteria Borrelia burgdorferi.
  • The researchers collected blood samples from 727 horses from various locations in Korea over a span of four years, from 2009 to 2013.
  • The collected data also included specific details about each horse’s age, gender, breed, and the region where the sample was collected. These details were considered for a thorough epidemiological analysis.

Findings

  • The Enzyme-Linked Immunosorbent Assay (ELISA) test was used to check for the presence of antibodies against B. burgdorferi in the horses’ blood, which indicates exposure to the bacteria – not necessarily an active infection.
  • The ELISA test revealed that out of the 727 horses, 38 of them (about 5.2% of the total) were seropositive, suggesting that they had been exposed to B. burgdorferi.
  • The researchers found statistically significant differences based on the breed and the geographical region where the samples were collected. The reason for these differences might be down to varying climate conditions and the prevalence of vector ticks in each area.

Polymerase Chain Reaction (PCR) Testing

  • In addition to the ELISA test, the research used 2 nested Polymerase Chain Reaction (PCR) tests to identify the presence of the DNA of B. burgdorferi which would indicate an active infection.
  • However, none of the samples tested through PCR showed a positive result for B. burgdorferi, suggesting none of the tested horses had an active infection during the period of the study.

Implications

  • The findings of the study imply that the tested horses might have been exposed to B. burgdorferi in the past. However, it doesn’t signify an ongoing bacterial presence or an active infection.
  • The study deemed it necessary to continue monitoring tick-borne diseases in horses in Korea given the anticipated rise in tick populations due to global warming.

Cite This Article

APA
Lee SH, Yun SH, Choi E, Park YS, Lee SE, Cho GJ, Kwon OD, Kwak D. (2016). Serological Detection of Borrelia burgdorferi among Horses in Korea. Korean J Parasitol, 54(1), 97-101. https://doi.org/10.3347/kjp.2016.54.1.97

Publication

The Korean journal of parasitology
ISSN: 1738-0006
NlmUniqueID: 9435800
Country: Korea (South)
Language: English
Volume: 54
Issue: 1
Pages: 97-101

Researcher Affiliations

Lee, Seung-Hun
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Yun, Sun-Hee
  • Division of Veterinary Service Lab, Institute of Public Health & Environment, Incheon 22320, Korea.
Choi, Eunsang
  • Smile Equine Clinic, Busan 46745, Korea.
Park, Yong-Soo
  • Korea National College of Agriculture and Fisheries, Jeonju 54874, Korea.
Lee, Sang-Eun
  • Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongju 28159, Korea.
Cho, Gil-Jae
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Kwon, Oh-Deog
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea.
Kwak, Dongmi
  • College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea ; Cardiovascular Research Institute, Kyungpook National University, Daegu 41566, Korea.

MeSH Terms

  • Animals
  • Antibodies, Bacterial / blood
  • Borrelia burgdorferi / physiology
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Horse Diseases / epidemiology
  • Horses
  • Lyme Disease / epidemiology
  • Lyme Disease / veterinary
  • Male
  • Republic of Korea / epidemiology

Conflict of Interest Statement

The authors report no conflict of interest related to this study.

References

This article includes 30 references
  1. Skotarczak B. Why are there several species of Borrelia burgdorferi sensu lato detected in dogs and humans?. Infect Genet Evol 2014 Apr;23:182-8.
    pubmed: 24613432doi: 10.1016/j.meegid.2014.02.014google scholar: lookup
  2. Moon S, Gwack J, Hwang KJ, Kwon D, Kim S, Noh Y, Roh J, Shin EH, Jeong K, Seok W, Youn SK. Autochthonous lyme borreliosis in humans and ticks in Korea.. Osong Public Health Res Perspect 2013 Feb;4(1):52-6.
    pmc: PMC3747679pubmed: 24159530doi: 10.1016/j.phrp.2012.12.001google scholar: lookup
  3. Butler CM, Houwers DJ, Jongejan F, van der Kolk JH. Borrelia burgdorferi infections with special reference to horses. A review.. Vet Q 2005 Dec;27(4):146-56.
    pubmed: 16402512
  4. Wang IN, Dykhuizen DE, Qiu W, Dunn JJ, Bosler EM, Luft BJ. Genetic diversity of ospC in a local population of Borrelia burgdorferi sensu stricto.. Genetics 1999 Jan;151(1):15-30.
    pmc: PMC1460459pubmed: 9872945doi: 10.1093/genetics/151.1.15google scholar: lookup
  5. Mannelli A, Bertolotti L, Gern L, Gray J. Ecology of Borrelia burgdorferi sensu lato in Europe: transmission dynamics in multi-host systems, influence of molecular processes and effects of climate change.. FEMS Microbiol Rev 2012 Jul;36(4):837-61.
    pubmed: 22091928doi: 10.1111/j.1574-6976.2011.00312.xgoogle scholar: lookup
  6. Skotarczak B, Wodecka B. Identification of Borrelia burgdorferi genospecies inducing Lyme disease in dogs from Western Poland.. Acta Vet Hung 2005;53(1):13-21.
    pubmed: 15782655doi: 10.1556/avet.53.2005.1.2google scholar: lookup
  7. Burbelo PD, Bren KE, Ching KH, Coleman A, Yang X, Kariu T, Iadarola MJ, Pal U. Antibody profiling of Borrelia burgdorferi infection in horses.. Clin Vaccine Immunol 2011 Sep;18(9):1562-7.
    pmc: PMC3165210pubmed: 21775514doi: 10.1128/cvi.05123-11google scholar: lookup
  8. Burgess EC, Mattison M. Encephalitis associated with Borrelia burgdorferi infection in a horse.. J Am Vet Med Assoc 1987 Dec 1;191(11):1457-8.
    pubmed: 3692996
  9. James FM, Engiles JB, Beech J. Meningitis, cranial neuritis, and radiculoneuritis associated with Borrelia burgdorferi infection in a horse.. J Am Vet Med Assoc 2010 Nov 15;237(10):1180-5.
    pubmed: 21073390doi: 10.2460/javma.237.10.1180google scholar: lookup
  10. Browning A, Carter SD, Barnes A, May C, Bennett D. Lameness associated with Borrelia burgdorferi infection in the horse.. Vet Rec 1993 Jun 12;132(24):610-1.
    pubmed: 8337810doi: 10.1136/vr.132.24.610google scholar: lookup
  11. Burgess EC, Gillette D, Pickett JP. Arthritis and panuveitis as manifestations of Borrelia burgdorferi infection in a Wisconsin pony.. J Am Vet Med Assoc 1986 Nov 15;189(10):1340-2.
    pubmed: 3793582
  12. Sears KP, Divers TJ, Neff RT, Miller WH Jr, McDonough SP. A case of Borrelia-associated cutaneous pseudolymphoma in a horse.. Vet Dermatol 2012 Apr;23(2):153-6.
    pubmed: 22029872doi: 10.1111/j.1365-3164.2011.01013.xgoogle scholar: lookup
  13. Borchers AT, Keen CL, Huntley AC, Gershwin ME. Lyme disease: a rigorous review of diagnostic criteria and treatment.. J Autoimmun 2015 Feb;57:82-115.
    pubmed: 25451629doi: 10.1016/j.jaut.2014.09.004google scholar: lookup
  14. Chengxu A, Yuxin W, Yongguo Z, Shaoshan W, Quicheng Q, Zhixue S, Deyou L, Dongquan C, Xiaodong L, Jienhua Z. Clinical manifestations and epidemiological characteristics of lyme disease in Hailin county, Heilongjiang Province, China.. Ann NewYork Acad Sci 1988;539:302–313.
  15. Tay ST, Kamalanathan M, Rohani MY. Borrelia burgdorferi (strain B. afzelii) antibodies among Malaysian blood donors and patients.. Southeast Asian J Trop Med Public Health 2002 Dec;33(4):787-93.
    pubmed: 12757227
  16. Kawabata M, Baba S, Iguchi K, Yamaguti N, Russell H. Lyme disease in Japan and its possible incriminated tick vector, Ixodes persulcatus.. J Infect Dis 1987 Nov;156(5):854.
    pubmed: 3655407doi: 10.1093/infdis/156.5.854google scholar: lookup
  17. Park KH, Chang WH, Schwan TG. Identification and characterization of Lyme disease spirochetes, Borrelia burgdorferi sensu lato, isolated in Korea.. J Clin Microbiol 1993 Jul;31(7):1831-7.
    pmc: PMC265641pubmed: 8349761doi: 10.1128/jcm.31.7.1831-1837.1993google scholar: lookup
  18. Jung BY, Gebeyehu EB, Seo MG, Byun JW, Kim HY, Kwak D. Prevalence of vector-borne diseases in shelter dogs in Korea.. Vet Rec 2012 Sep 8;171(10):249.
    pubmed: 22798342doi: 10.1136/vr.100650google scholar: lookup
  19. Lim S, Irwin PJ, Lee S, Oh M, Ahn K, Myung B, Shin S. Comparison of selected canine vector-borne diseases between urban animal shelter and rural hunting dogs in Korea.. Parasit Vectors 2010 Apr 8;3(1):32.
    pmc: PMC2860352pubmed: 20377869doi: 10.1186/1756-3305-3-32google scholar: lookup
  20. Kim HC, Han SH, Chong ST, Klein TA, Choi CY, Nam HY, Chae HY, Lee H, Ko S, Kang JG, Chae JS. Ticks collected from selected mammalian hosts surveyed in the Republic of Korea during 2008-2009.. Korean J Parasitol 2011 Sep;49(3):331-5.
    pmc: PMC3210857pubmed: 22072840doi: 10.3347/kjp.2011.49.3.331google scholar: lookup
  21. Lee SH, Lee SE, Seo MG, Goo YK, Cho KH, Cho GJ, Kwon OD, Kwak D, Lee WJ. Evidence of Toxoplasma gondii exposure among horses in Korea.. J Vet Med Sci 2014 Dec;76(12):1663-5.
    pmc: PMC4300387pubmed: 25649954doi: 10.1292/jvms.14-0353google scholar: lookup
  22. Jung BY, Lee KW, Ha TY. Seroprevalence of Leptospira spp. in clinically healthy racing horses in Korea.. J Vet Med Sci 2010 Feb;72(2):197-201.
    pubmed: 19942812doi: 10.1292/jvms.09-0273google scholar: lookup
  23. Chae JS, Adjemian JZ, Kim HC, Ko S, Klein TA, Foley J. Predicting the emergence of tick-borne infections based on climatic changes in Korea.. Vector Borne Zoonotic Dis 2008 Apr;8(2):265-75.
    pubmed: 18429692doi: 10.1089/vbz.2007.0190google scholar: lookup
  24. Thrusfield M. Veterinary Epidemiology. 3rd ed. Oxford, UK: Blackwell Publishing; 2005. pp. 228–246.
  25. Chandrashekar R, Daniluk D, Moffitt S, Lorentzen L, Williams J. Serologic diagnosis of equine borreliosis: evaluation of an inclinic enzyme-linked immunosorbent assay (SNAP 4Dx). Intern J Appl Res Vet Med 2008;6:145–150.
  26. Hansen MG, Christoffersen M, Thuesen LR, Petersen MR, Bojesen AM. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in Danish horses.. Acta Vet Scand 2010 Jan 18;52(1):3.
    pmc: PMC2818635pubmed: 20082693doi: 10.1186/1751-0147-52-3google scholar: lookup
  27. Chu CY, Jiang BG, Liu W, Zhao QM, Wu XM, Zhang PH, Zhan L, Yang H, Cao WC. Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang, south-east China.. J Med Microbiol 2008 Aug;57(Pt 8):980-985.
    pubmed: 18628499doi: 10.1099/jmm.0.47663-0google scholar: lookup
  28. Reiczigel J, Földi J, Ozsvári L. Exact confidence limits for prevalence of a disease with an imperfect diagnostic test.. Epidemiol Infect 2010 Nov;138(11):1674-8.
    pubmed: 20196903doi: 10.1017/s0950268810000385google scholar: lookup
  29. Léger E, Vourc'h G, Vial L, Chevillon C, McCoy KD. Changing distributions of ticks: causes and consequences.. Exp Appl Acarol 2013 Feb;59(1-2):219-44.
    pubmed: 23015121doi: 10.1007/s10493-012-9615-0google scholar: lookup
  30. Divers TJ, Grice AL, Mohammed HO, Glaser AL, Wagner B. Changes in Borrelia burgdorferi ELISA antibody over time in both antibiotic treated and untreated horses.. Acta Vet Hung 2012 Dec;60(4):421-9.
    pubmed: 23160024doi: 10.1556/avet.2012.036google scholar: lookup

Citations

This article has been cited 17 times.
  1. Kašpárková N, Bártová E, Žákovská A, Budíková M, Sedlák K. Antibodies against Borrelia burgdorferi Sensu Lato in Clinically Healthy and Sick Horses: First Report from the Czech Republic. Microorganisms 2023 Jun 29;11(7).
    doi: 10.3390/microorganisms11071706pubmed: 37512879google scholar: lookup
  2. Seo HJ, Truong AT, Kim KH, Lim JY, Min S, Kim HC, Yoo MS, Yoon SS, Klein TA, Cho YS. Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea. Pathogens 2021 Aug 24;10(9).
    doi: 10.3390/pathogens10091069pubmed: 34578102google scholar: lookup
  3. Subbiah M, Thirumalapura N, Thompson D, Kuchipudi SV, Jayarao B, Tewari D. Detection of Anaplasma Phagocytophilum in Horses With Suspected Tick-Borne Disease in Northeastern United States by Metagenomic Sequencing. Front Vet Sci 2021;8:673193.
    doi: 10.3389/fvets.2021.673193pubmed: 34179165google scholar: lookup
  4. Costa SCL, de Souza Freitas J, Carvalho FS, Pereira MJS, Cordeiro MD, da Fonseca AH, Gomes Jusi MM, Machado RZ, Munhoz AD. Frequency and factors associated of potential zoonotic pathogens (Borrelia spp., Rickettsia spp., Leishmania spp., and Anaplasma phagocytophilum) in equids in the state of Bahia, Brazil. Parasit Vectors 2021 May 22;14(1):275.
    doi: 10.1186/s13071-021-04777-4pubmed: 34022939google scholar: lookup
  5. Acharya D, Park JH. Seroepidemiologic Survey of Lyme Disease among Forestry Workers in National Park Offices in South Korea. Int J Environ Res Public Health 2021 Mar 12;18(6).
    doi: 10.3390/ijerph18062933pubmed: 33809327google scholar: lookup
  6. Kim SY, Kim TK, Kim TY, Lee HI. Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea. Pathogens 2020 Oct 22;9(11).
    doi: 10.3390/pathogens9110866pubmed: 33105824google scholar: lookup
  7. Laamari A, Azzag N, Tennah S, Derdour SY, China B, Boꯚllah R, Ghalmi F. Seroprevalence of Antibodies Against Anaplasma Phagocytophilum and Borrelia Burgdorferi in Horses (Equus Caballus) from Northern Algeria. J Vet Res 2020 Sep;64(3):413-419.
    doi: 10.2478/jvetres-2020-0045pubmed: 32984632google scholar: lookup
  8. Seo MG, Kwon OD, Kwak D. Molecular Identification of Borrelia afzelii from Ticks Parasitizing Domestic and Wild Animals in South Korea. Microorganisms 2020 Apr 29;8(5).
    doi: 10.3390/microorganisms8050649pubmed: 32365723google scholar: lookup
  9. Lee SH, Goo YK, Geraldino PJL, Kwon OD, Kwak D. Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea. Pathogens 2019 Dec 6;8(4).
    doi: 10.3390/pathogens8040289pubmed: 31817729google scholar: lookup
  10. Chae JB, Cho YS, Cho YK, Kang JG, Shin NS, Chae JS. Epidemiological Investigation of Tick Species from Near Domestic Animal Farms and Cattle, Goat, and Wild Boar in Korea. Korean J Parasitol 2019 Jun;57(3):319-324.
    doi: 10.3347/kjp.2019.57.3.319pubmed: 31284358google scholar: lookup
  11. Bae JM. Horses as a Potential Reservoir of Lyme Borreliosis in Jeju-do, Korea. J Prev Med Public Health 2018 Jul;51(4):213-214.
    doi: 10.3961/jpmph.18.081pubmed: 30071709google scholar: lookup
  12. Kang JG, Chae JB, Cho YK, Jo YS, Shin NS, Lee H, Choi KS, Yu DH, Park J, Park BK, Chae JS. Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea. Am J Trop Med Hyg 2018 Apr;98(4):1061-1068.
    doi: 10.4269/ajtmh.17-0380pubmed: 29436346google scholar: lookup
  13. Izac JR, Oliver LD Jr, Earnhart CG, Marconi RT. Identification of a defined linear epitope in the OspA protein of the Lyme disease spirochetes that elicits bactericidal antibody responses: Implications for vaccine development. Vaccine 2017 May 31;35(24):3178-3185.
    doi: 10.1016/j.vaccine.2017.04.079pubmed: 28479174google scholar: lookup
  14. Yoo JR, Kim M, Jeong W, Song SW. First Detection of Seroreactivity to Lyme Borreliosis in Agricultural Workers from a Non-Endemic Region of South Korea. Yonsei Med J 2025 Jul;66(7):456-458.
    doi: 10.3349/ymj.2024.0376pubmed: 40551595google scholar: lookup
  15. Kim B, Lee YJ, Lee HI, Kwak D, Seo MG. Surveillance of Vector-Borne Zoonotic Diseases in South Korea: Uncovering Novel Pathogen Carriers Among Rodents and Mites Nationwide. Transbound Emerg Dis 2024;2024:5544660.
    doi: 10.1155/2024/5544660pubmed: 40303086google scholar: lookup
  16. Choi KS, Hwang S, Kim MC, Cho HC, Park YJ, Ji MJ, Han SW, Chae JS. Molecular surveillance of zoonotic pathogens from wild rodents in the Republic of Korea. PLoS Negl Trop Dis 2024 Jul;18(7):e0012306.
    doi: 10.1371/journal.pntd.0012306pubmed: 38976750google scholar: lookup
  17. Gehlen H, Inerle K, Bartel A, Stöckle SD, Ulrich S, Briese B, Straubinger RK. Seroprevalence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum Infections in German Horses. Animals (Basel) 2023 Jun 14;13(12).
    doi: 10.3390/ani13121984pubmed: 37370494google scholar: lookup
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