Seroprevalence and molecular characterization of West Nile Virus in Egypt.
Abstract: West Nile Virus (WNV) is a flavivirus, mosquito-borne infection and have public health importance worldwide. WNV infection have highly significant impact on animal and human health. The virus has been detected serologically in Egypt among equids. Therefore, the aim of the present study to investigate the serological situation of WNV among horse in north of Egypt and identification of WNV in vector. The serological survey was conducted on 500 serum samples that collected from horses from four governorates at north of Egypt. The infection rate was non-significant differed between four localities and the highest rate was reported in Qalyubia governorate (25.5 %) in comparison with other areas. Moreover, the WNV RNA was detected in mosquitoes and the obtained WNV sequence showed high similarity with Eg101 strain and characterized as lineage 1. The obtained findings confirm the circulation of WNV in mosquitoes and animals in Egypt.
Copyright © 2020 Elsevier Ltd. All rights reserved.
Publication Date: 2020-03-24 PubMed ID: 32289595DOI: 10.1016/j.cimid.2020.101473Google Scholar: Lookup
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Summary
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The research conducts a serological examination of the West Nile Virus (WNV) in horses in the northern regions of Egypt, also identifying the virus in mosquito vectors, with findings confirming its circulation among this population.
Objective of the Study
- The main objective of the study was to understand the serological situation of the West Nile Virus amongst horses in the northern region of Egypt. It also aimed to identify the presence of WNV within mosquito vectors in the area.
Research Methodology
- A serological survey was undertaken on 500 serum samples. These samples were collected from horses spread across four governorates in Northern Egypt: Alexandria, Beheira, Qalyubia, and Gharbia.
- The study then compared the infection rates across the four areas, identifying the highest rate of infection in the Qalyubia governorate.
- The researchers further detected the presence of WNV’s genetic material (RNA) in mosquitoes within the region.
Key Findings
- Infection rate differences across the four localities were non-significant. That is, the rate of infection was generally consistent across the tested regions in North Egypt.
- However, the greatest rate of infection was found in Qalyubia governorate with a prevalence of 25.5% when compared to other areas. This implies that roughly a quarter of the horses tested in this region carried WNV.
- Furthermore, through molecular analysis, WNV RNA was successfully identified in mosquitoes in the region, thereby confirming them as primary vector hosts.
- The molecular characterization of the detected WNV revealed high similarities with the Eg101 strain and was categorized as lineage 1. It means the WNV found was similar to a notable strain known as Eg101 and belongs to a specific classification (lineage 1).
- Finally, these results confirm the circulation of WNV among mosquitoes and equine population in the region, emphasizing its potential threat to animal and human health.
Cite This Article
APA
Selim A, Radwan A, Arnaout F.
(2020).
Seroprevalence and molecular characterization of West Nile Virus in Egypt.
Comp Immunol Microbiol Infect Dis, 71, 101473.
https://doi.org/10.1016/j.cimid.2020.101473 Publication
Researcher Affiliations
- Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt. Electronic address: Abdelfattah.selim@fvtm.bu.edu.eg.
- Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt.
- Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Egypt.
Conflict of Interest Statement
Declaration of Competing Interest The authors have no conflicts of interest to declare.
Citations
This article has been cited 13 times.- Radwan IT, Sayed-Ahmed MZ, Ghazawy NA, Alqahtani SS, Ahmad S, Alam N, Alkhaibari AM, Ali MS, Selim A, AbdelFattah EA. Effect of nanostructure lipid carrier of methylene blue and monoterpenes as enzymes inhibitor for Culex pipiens.. Sci Rep 2023 Aug 2;13(1):12522.
- Selim A, Marawan MA, Abdelhady A, Alshammari FA, Alqhtani AH, Ba-Awadh HA, Olarinre IO, Swelum AA. Coxiella burnetii and its risk factors in cattle in Egypt: a seroepidemiological survey.. BMC Vet Res 2023 Jan 31;19(1):29.
- Selim A, Shoulah S, Alsubki RA, Albohairy FM, Attia KA, Kimiko I. Sero-survey of bovine herpes virus-1 in dromedary camels and associated risk factors.. BMC Vet Res 2022 Sep 30;18(1):362.
- Selim A, Alsubki RA, Albohairy FM, Attia KA, Kimiko I. A survey of bluetongue infection in one-humped camels (Camelus Dromedarius); seroprevalence and risk factors analysis.. BMC Vet Res 2022 Aug 22;18(1):322.
- Fang Y, Khater EIM, Xue JB, Ghallab EHS, Li YY, Jiang TG, Li SZ. Epidemiology of Mosquito-Borne Viruses in Egypt: A Systematic Review.. Viruses 2022 Jul 20;14(7).
- Selim A, Attia KA, Alsubki RA, Albohairy F, Kimiko I, Said MB. The first study on the seroprevalence of Anaplasma spp. in small ruminants and assessment of associated risk factors in North Egypt.. Vet World 2022 May;15(5):1221-1227.
- Selim A, Weir W, Khater H. Prevalence and risk factors associated with tropical theileriosis in Egyptian dairy cattle.. Vet World 2022 Apr;15(4):919-924.
- Radwan IT, Baz MM, Khater H, Selim AM. Nanostructured Lipid Carriers (NLC) for Biologically Active Green Tea and Fennel Natural Oils Delivery: Larvicidal and Adulticidal Activities against Culex pipiens.. Molecules 2022 Mar 17;27(6).
- Marawan MA, Alouffi A, El Tokhy S, Badawy S, Shirani I, Dawood A, Guo A, Almutairi MM, Alshammari FA, Selim A. Bovine Leukaemia Virus: Current Epidemiological Circumstance and Future Prospective.. Viruses 2021 Oct 27;13(11).
- Selim A, Shoulah S, Abdelhady A, Alouffi A, Alraey Y, Al-Salem WS. Seroprevalence and Risk Factors Associated with Canine Leishmaniasis in Egypt.. Vet Sci 2021 Oct 15;8(10).
- Olufemi OT, Barba M, Daly JM. A Scoping Review of West Nile Virus Seroprevalence Studies among African Equids.. Pathogens 2021 Jul 15;10(7).
- Selim A, Khater H, Almohammed HI. A recent update about seroprevalence of ovine neosporosis in Northern Egypt and its associated risk factors.. Sci Rep 2021 Jul 7;11(1):14043.
- Selim A, Manaa EA, Alanazi AD, Alyousif MS. Seroprevalence, Risk Factors and Molecular Identification of Bovine Leukemia Virus in Egyptian Cattle.. Animals (Basel) 2021 Jan 27;11(2).
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