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Seroprevalence of African horse sickness in selected donkey populations in Namibia.
Abstract: African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the Reoviridae family and genus Orbivirus. AHS is an endemic disease that is responsible for the death of a high number of horses every year in Namibia. At present, there is no information on the prevalence and distribution of AHS virus (AHSV) serotypes in the different regions of Namibia. Therefore, this survey aimed to fill this knowledge gap by investigating the AHSV seroprevalence in Namibian donkeys. Methods: A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using acommercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response. Results: Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8. Conclusions: Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.
Copyright: © Molini, et al.
Publication Date: 2020-05-31 PubMed ID: 32636601PubMed Central: PMC7311865DOI: 10.14202/vetworld.2020.1005-1009Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This study investigates the prevalence and distribution of African horse sickness virus (AHSV) serotypes in donkey populations across different regions in Namibia. Findings show that various serotypes of the virus have circulated in different regions, with AHSV-9 and AHSV-6 being the most prevalent.
Methodology
- The researchers collected 260 blood samples from donkeys aged between 3 and 5 years from various regions of Namibia.
- These samples were screened for AHSV-specific immunoglobulin G antibodies using a commercial enzyme-linked immunosorbent assay kit.
- Samples positive for AHSV antibodies were then evaluated via a serum neutralization assay to determine the specific AHSV serotype causing the immune response.
Results
- The seroprevalence of antibodies against AHSV in Namibian donkeys was found to be 63.5%.
- Analyzed by region, the prevalence of AHSV was significantly higher in the northern region (64%) compared to the southern region (36%).
- A larger number of donkeys had antibodies against serotypes AHSV-6 (37.8%) and AHSV-9 (37.8%), with these serotypes more common in the northern regions.
- No donkeys tested positive for AHSV-8 in this study.
Conclusions
- All serotypes of AHSV have been found to either currently or previously circulate in Namibia, except for AHSV-8.
- Specifically, serotypes AHSV-1, -2, -3, -4, -5, -6, and -9 have been found in the north, while AHSV-1, -4, -5, -6, -7, and -9 have been found in the south.
- The most prevalent serotypes detected in donkeys during this study were AHSV-9 and AHSV-6.
- Results from the serum neutralization assay suggested exposure to multiple serotypes in donkeys from the Kavango East, Kavango West, and Ohangwena regions, indicating possible co-circulation of various strains in Namibia.
Cite This Article
APA
Molini U, Zaccaria G, Kandiwa E, Mushonga B, Khaiseb S, Ntahonshikira C, Chiwome B, Baines I, Madzingira O, Savini G, D'Alterio N.
(2020).
Seroprevalence of African horse sickness in selected donkey populations in Namibia.
Vet World, 13(5), 1005-1009.
https://doi.org/10.14202/vetworld.2020.1005-1009 Publication
Researcher Affiliations
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo Italy.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Department of Virology, Central Veterinary Laboratory, 24 Goethe Street, Windhoek, Namibia.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Department of Pathobiology, School of Veterinary Medicine, Faculty of Agriculture and Natural Resources, University of Namibia, Neudamm Campus, Namibia.
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo Italy.
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" 64100 Teramo Italy.
References
This article includes 19 references
- Zientara S, Weyer C.T, Lecollinet S. African horse sickness. Rev. Sci. Tech. 2015;34(2):315–327.
- Mellor P.S, Hamblin C. African horse sickness. Vet. Res. 2004;35(4):445–466.
- Sailleau C, Hamblin C, Paweska J.T, Zientara S. Identification and differentiation of nine African horse sickness virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2. J. Gen. Virol. 2000;81(3):831–837.
- Maclachlan N.J, Guthrie A.J. Re-emergence of bluetongue, African horse sickness and other orbivirus diseases. Vet. Res. 2010;41(6):35.
- Carpenter S, Mellor P.S, Fall A.G, Garros C, Venter G.J. African horse sickness virus:History, transmission, and current status. Annu. Rev. Entomol. 2017;31(62):343–358.
- Ngoveni H.G, van Schalkwyk A, Koekemoer J.J.O. Evidence of intragenic recombination in African horse sickness virus. Viruses 2019;11(7):654.
- Howell P.G, Groenewald D, Visage C.W, Bosman A.M, Coetzer J.A, Guthrie A.J. The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa. Onderstepoort J. Vet. Res. 2002;69(1):79–93.
- Scacchia M, Molini U, Marruchella G, Maseke A, Bortone G, Cosseddu G.M, Monaco F, Savini G, Pini A. African horse sickness outbreaks in Namibia from 2006 to 2013:Clinical, pathological and molecular findings. Vet. Ital. 2015;51(2):123–130.
- Teshome M, Addis M, Temesgen W. Seroprevalence and risk factors of African horse sickness in mules and donkeys in selected sites of West Amhara region, Ethiopia. Afr. J. Microbiol. Res. 2012;6(19):4146–4151.
- Gordon S.J.G, Bolwell C, Rogers C.W, Musuka G, Kelly P, Guthrie A, Mellor P.S, Hamblin C. The seroprevalence and sero-incidence of African horse sickness and equine encephalosis in selected horse and donkey populations in Zimbabwe. Onderstepoort J. Vet Res. 2017;84(1):e1–e5.
- Von Teichman B.F, Dungu B, Smit T.K. In vivo cross-protection to African horse sickness Serotypes 5 and 9 after vaccination with Serotypes 8 and 6. Vaccine 2010;28(39):6505–6517.
- Dennis S.J, Meyers A.E, Hitzeroth I.I, Rybicki E.P. African horse sickness:A review of current understanding and vaccine development. Viruses 2019;11(9):E844.
- Molini U, Marruchella G, Maseke A, Ronchi G.F, Di Ventura M, Salini R, Scacchia M, Pini A. Immunization of horses with a polyvalent live-attenuated African horse sickness vaccine:Serological response and disease occurrence under field conditions. Trials Vaccinol. 2015;4(2015):24–28.
- Weyer C.T, Grewar J.D, Burger P, Joone C, Lourens C, MacLachlan N.J, Guthrie A.J. Dynamics of African horse sickness virus nucleic acid and antibody in horses following immunization with a commercial polyvalent live attenuated vaccine. Vaccine 2017;35(18):2504–2510.
- Weyer C.T, Grewar J.D, Burger P, Rossouw E, Lourens C, Joone C, le Grange M, Coetzee P, Venter E, Martin D.P, MacLachlan N.J, Guthrie A.J. African horse sickness caused by genome reassortment and reversion to virulence of live, attenuated vaccine viruses, South Africa, 2004-2014. Emerg. Infect. Dis. 2016;22(12):2087–2096.
- Vermaak E, Paterson D.J, Conradie A, Theron J. Directed genetic modification of African horse sickness virus by reverse genetics. S. Afr. J. Sci. 2015;111(7-8):1–8.
- MacLachlan N.J, Balasuriya U.B, Davis N.L, Collier M, Johnston R.E, Ferraro G.L, Guthrie A.J. Experiences with new generation vaccines against equine viral arteritis, West Nile disease and African horse sickness. Vaccine 2007;25(30):5577–5582.
- Domingo E. Mechanisms of viral emergence. Vet. Res. 2010;41(6):38.
- Chare E.R, Gould E.A, Holmes E.C. Phylogenetic analysis reveals a low rate of homologous recombination in negative-sense RNA viruses. J. Gen. Virol. 2003;8(Pt 10):2691–2703.
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