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Iranian journal of parasitology2012; 6(2); 64-68;

Seroprevalence of Neospora spp. in Horses in North East of Iran.

Abstract: Neospora caninum, an obligate intracellular protozoan parasite, is recognized as a major cause of abortion in cattle, while limited information is presently available on the seroprevalence of Neospora antibodies in horses' worldwide. The aim of the present study was to determine serologic prevalence of Neospora infection in horses in Iran. Methods: Sera from 150 horses from Mashhad suburb in Razavi Khorasan Province, northeast Iran were examined for antibodies to Neospora spp. using Neospora modified direct agglutination test (N-MAT). Results: Antibodies to this parasite were detected in 45 (30%) of the examined serum samples. Thirty four percent of the samples had titer of 1:40 while then reduced to 30% when 1:80 serum dilution was applied as significant cut off titer. Conclusions: This study is the first investigation carried out on the Neospora in horses in Iran and indicates that horses in Iran are exposed to this parasite.
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Publication Date: 2012-02-22 PubMed ID: 22347290PubMed Central: PMC3279880
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study revolves around investigating the prevalence of the parasitic infection caused by Neospora caninum in horses located in North East Iran. With 150 subjects involved, it was found that 30% of the horses were exposed to this parasite.

Objective

The primary objective was to identify the seroprevalence of Neospora infection within horses in Iran. Although more prevalent in cattle, the information regarding Neospora antibodies in horses is limited globally. This research therefore contributes valuable data in understanding the exposure of horses to the Neospora parasite in Iran.

Methodology

  • The research involved testing sera samples acquired from 150 horses located in Mashhad suburb of Razavi Khorasan Province, Northeast Iran.
  • These samples were examined for the presence of Neospora antibodies using the Neospora modified direct agglutination test (N-MAT).
  • The N-MAT is a reliable method to detect Neospora-specific antibodies in sera samples.

Results

  • Out of the 150 samples, antibodies linked to the Neospora parasite were identified in 45 samples, which constitutes 30% of the total.
  • 34% of these positive samples had a titer value of 1:40. The titer is a measure of the concentration of antibodies in the blood.
  • The percentage reduced to 30% when 1:80 serum dilution was applied. Here, serum dilution refers to the practice of lessening the concentration of the antiserum, in order to differentiate between high and low levels of antibodies.

Conclusion

  • This is the first study that delves into the prevalence of Neospora infection in horses in Iran. Therefore, it plays a critical role in providing initial data regarding the extent of horses’ exposure to this parasitic infection in the said region.
  • The research concluded that a significant percentage of horses in Iran, approximately 30%, were found to be exposed to the Neospora parasite, indicating the need for preventive and control steps.

Cite This Article

APA
Hosseini M, Moraveji M, Tahamtan Y, Rahimian A, Mohammadi G, Namavari M. (2012). Seroprevalence of Neospora spp. in Horses in North East of Iran. Iran J Parasitol, 6(2), 64-68.

Publication

Iranian journal of parasitology
ISSN: 2008-238X
NlmUniqueID: 101464309
Country: Iran
Language: English
Volume: 6
Issue: 2
Pages: 64-68

Researcher Affiliations

Hosseini, Mh
  • Razi Vaccine and Serum Research Institute, Shiraz, Iran.
Moraveji, M
    Tahamtan, Y
      Rahimian, A
        Mohammadi, Gh
          Namavari, Mm

            References

            This article includes 27 references
            1. Bjerkås I, Mohn SF, Presthus J. Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs.. Z Parasitenkd 1984;70(2):271-4.
              pubmed: 6426185doi: 10.1007/bf00942230google scholar: lookup
            2. Barr BC, Anderson ML, Dubey JP, Conrad PA. Neospora-like protozoal infections associated with bovine abortions.. Vet Pathol 1991 Mar;28(2):110-6.
              pubmed: 2063512doi: 10.1177/030098589102800202google scholar: lookup
            3. Barr BC, Anderson ML, Woods LW, Dubey JP, Conrad PA. Neospora-like protozoal infections associated with abortion in goats.. J Vet Diagn Invest 1992 Jul;4(3):365-7.
              pubmed: 1515507doi: 10.1177/104063879200400331google scholar: lookup
            4. Cole RA, Lindsay DS, Blagburn BL, Sorjonen DC, Dubey JP. Vertical transmission of Neospora caninum in dogs.. J Parasitol 1995 Apr;81(2):208-11.
              pubmed: 7707195
            5. Dubey JP, Lindsay DS. Neospora caninum induced abortion in sheep.. J Vet Diagn Invest 1990 Jul;2(3):230-3.
              pubmed: 2094452doi: 10.1177/104063879000200316google scholar: lookup
            6. Dubey JP, Lindsay DS, Lipscomb TP. Neosporosis in cats.. Vet Pathol 1990 Sep;27(5):335-9.
              pubmed: 2238386doi: 10.1177/030098589002700505google scholar: lookup
            7. Dubey JP, Porterfield ML. Neospora caninum (Apicomplexa) in an aborted equine fetus.. J Parasitol 1990 Oct;76(5):732-4.
              pubmed: 2213418
            8. Dubey JP, Mitchell SM, Morrow JK, Rhyan JC, Stewart LM, Granstrom DE, Romand S, Thulliez P, Saville WJ, Lindsay DS. Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming.. J Parasitol 2003 Aug;89(4):716-20.
              pubmed: 14533680doi: 10.1645/ge-66rgoogle scholar: lookup
            9. Vardeleon D, Marsh AE, Thorne JG, Loch W, Young R, Johnson PJ. Prevalence of Neospora hughesi and Sarcocystis neurona antibodies in horses from various geographical locations.. Vet Parasitol 2001 Feb 26;95(2-4):273-82.
              pubmed: 11223207doi: 10.1016/s0304-4017(00)00393-9google scholar: lookup
            10. Cheadle MA, Lindsay DS, Rowe S, Dykstra CC, Williams MA, Spencer JA, Toivio-Kinnucan MA, Lenz SD, Newton JC, Rolsma MD, Blagburn BL. Prevalence of antibodies to Neospora sp. in horses from Alabama and characterisation of an isolate recovered from a naturally infected horse [corrected].. Int J Parasitol 1999 Oct;29(10):1537-43.
              pubmed: 10608440doi: 10.1016/s0020-7519(99)00140-xgoogle scholar: lookup
            11. Gupta GD, Lakritz J, Kim JH, Kim DY, Kim JK, Marsh AE. Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea.. Vet Parasitol 2002 Jun 26;106(3):193-201.
              pubmed: 12062508doi: 10.1016/s0304-4017(02)00064-xgoogle scholar: lookup
            12. Pitel PH, Pronost S, Romand S, Thulliez P, Fortier G, Ballet JJ. Prevalence of antibodies to Neospora caninum in horses in France.. Equine Vet J 2001 Mar;33(2):205-7.
              pubmed: 11266072doi: 10.1111/j.2042-3306.2001.tb00602.xgoogle scholar: lookup
            13. Pitel PH, Romand S, Pronost S, Foucher N, Gargala G, Maillard K, Thulliez P, Collobert-Laugier C, Tainturier D, Fortier G, Ballet JJ. Investigation of Neospora sp. antibodies in aborted mares from Normandy, France.. Vet Parasitol 2003 Dec 1;118(1-2):1-6.
              pubmed: 14651869doi: 10.1016/j.vetpar.2003.10.007google scholar: lookup
            14. Ciaramella P, Corona M, Cortese L, Piantedosi D, Santoro D, Di Loria A, Rigato R. Seroprevalence of Neospora spp. in asymptomatic horses in Italy.. Vet Parasitol 2004 Aug 13;123(1-2):11-5.
              pubmed: 15265566doi: 10.1016/j.vetpar.2004.05.022google scholar: lookup
            15. Nourollahi Fard SR, Khalili M, Aminzadeh A. Prevalence of antibodies to Neospora Caninum in cattle in Kerman province, South East Iran. Veterinarski Arhiv 2008;78(3):253–259.
            16. Razmi GR, Mohammadi GR, Garrosi T, Farzaneh N, Fallah AH, Maleki M. Seroepidemiology of Neospora caninum infection in dairy cattle herds in Mashhad area, Iran.. Vet Parasitol 2006 Jan 30;135(2):187-9.
              pubmed: 16289861doi: 10.1016/j.vetpar.2005.09.004google scholar: lookup
            17. Sadrebazzaz A, Haddadzadeh H, Esmailnia K, Habibi G, Vojgani M, Hashemifesharaki R. Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran.. Vet Parasitol 2004 Oct 5;124(3-4):201-4.
              pubmed: 15381300doi: 10.1016/j.vetpar.2004.06.027google scholar: lookup
            18. Malmasi A, Hosseininejad M, Haddadzadeh H, Badii A, Bahonar A. Serologic study of anti-Neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in Tehran, Iran.. Parasitol Res 2007 Apr;100(5):1143-5.
              pubmed: 17120042doi: 10.1007/s00436-006-0385-7google scholar: lookup
            19. Haddadzadeh HR, Sadrebazzaz A, Malmasi A, Talei Ardakani H, Khazraii Nia P, Sadreshirazi N. Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran.. Parasitol Res 2007 Nov;101(6):1563-5.
              pubmed: 17687566doi: 10.1007/s00436-007-0678-5google scholar: lookup
            20. Sadrebazzaz A, Haddadzadeh H, Shayan P. Seroprevalence of Neospora caninum and Toxoplasma gondii in camels (Camelus dromedarius) in Mashhad, Iran.. Parasitol Res 2006 May;98(6):600-1.
              pubmed: 16425066doi: 10.1007/s00436-005-0118-3google scholar: lookup
            21. Packham AE, Sverlow KW, Conrad PA, Loomis EF, Rowe JD, Anderson ML, Marsh AE, Cray C, Barr BC. A modified agglutination test for Neospora caninum: development, optimization, and comparison to the indirect fluorescent-antibody test and enzyme-linked immunosorbent assay.. Clin Diagn Lab Immunol 1998 Jul;5(4):467-73.
              pmc: PMC95601pubmed: 9665950doi: 10.1128/cdli.5.4.467-473.1998google scholar: lookup
            22. Villalobos EM, Ueno TE, de Souza SL, Cunha EM, do Carmo Custódio de Souza Hunold Lara M, Gennari SM, Soares RM. Association between the presence of serum antibodies against Neospora spp. and fetal loss in equines.. Vet Parasitol 2006 Dec 20;142(3-4):372-5.
              pubmed: 16962708doi: 10.1016/j.vetpar.2006.07.016google scholar: lookup
            23. Lindsay DS. Neosporosis: an emerging protozoal disease of horses.. Equine Vet J 2001 Mar;33(2):116-8.
              pubmed: 11266059doi: 10.1111/j.2042-3306.2001.tb00588.xgoogle scholar: lookup
            24. Marsh AE, Howe DK, Wang G, Barr BC, Cannon N, Conrad PA. Differentiation of Neospora hughesi from Neospora caninum based on their immunodominant surface antigen, SAG1 and SRS2.. Int J Parasitol 1999 Oct;29(10):1575-82.
              pubmed: 10608444doi: 10.1016/s0020-7519(99)00120-4google scholar: lookup
            25. Hoane JS, Yeargan MR, Stamper S, Saville WJ, Morrow JK, Lindsay DS, Howe DK. Recombinant NhSAG1 ELISA: a sensitive and specific assay for detecting antibodies against Neospora hughesi in equine serum.. J Parasitol 2005 Apr;91(2):446-52.
              pubmed: 15986623doi: 10.1645/ge-395rgoogle scholar: lookup
            26. Dubey JP, Romand S, Thulliez P, Kwok OC, Shen SK, Gamble HR. Prevalence of antibodies to Neospora caninum in horses in North America.. J Parasitol 1999 Oct;85(5):968-9.
              pubmed: 10577738
            27. Corbellini LG, Smith DR, Pescador CA, Schmitz M, Correa A, Steffen DJ, Driemeier D. Herd-level risk factors for Neospora caninum seroprevalence in dairy farms in southern Brazil.. Prev Vet Med 2006 May 17;74(2-3):130-41.
              pubmed: 16343669doi: 10.1016/j.prevetmed.2005.11.004google scholar: lookup

            Citations

            This article has been cited 5 times.
            1. Rahmani SS, Malekifard F, Tavassoli M. Neospora caninum, a cause of abortion in donkeys (Equus asinus) in Iran. Parasitol Res 2022 Jan;121(1):367-372.
              doi: 10.1007/s00436-021-07383-6pubmed: 34811588google scholar: lookup
            2. Khamesipour F, Taktaz-Hafshejani T, Tebit KE, Razavi SM, Hosseini SR. Prevalence of endo- and ecto-parasites of equines in Iran: A systematic review. Vet Med Sci 2021 Jan;7(1):25-34.
              doi: 10.1002/vms3.321pubmed: 32644306google scholar: lookup
            3. Zhou M, Cao S, Sevinc F, Sevinc M, Ceylan O, Liu M, Wang G, Moumouni PF, Jirapattharasate C, Suzuki H, Nishikawa Y, Xuan X. Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey. J Vet Med Sci 2017 Jan 10;78(12):1877-1881.
              doi: 10.1292/jvms.16-0234pubmed: 27523504google scholar: lookup
            4. Tavalla M, Sabaghan M, Abdizadeh R, Khademvatan S, Rafiei A, Razavi Piranshahi A. Seroprevalence of Toxoplasma gondii and Neospora spp. Infections in Arab Horses, Southwest of Iran. Jundishapur J Microbiol 2015 Mar;8(3):e14939.
              doi: 10.5812/jjm.14939pubmed: 25834714google scholar: lookup
            5. Sazmand A, Bahari A, Papi S, Otranto D. Parasitic diseases of equids in Iran (1931-2020): a literature review. Parasit Vectors 2020 Nov 19;13(1):586.
              doi: 10.1186/s13071-020-04472-wpubmed: 33213507google scholar: lookup
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