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Serosal injury in the equine jejunum and ascending colon after ischemia-reperfusion or intraluminal distention and decompression.
Abstract: To document morphologic changes that occur in equine intestinal serosa after experimentally induced ischemia and subsequent reperfusion (jejunum, ascending colon) or after intraluminal distention and decompression (jejunum). Methods: Morphologic effects of ischemia-reperfusion or intraluminal distention-decompression determined on the serosal layer of the equine jejunum. The large colon serosa was evaluated after ischemia-reperfusion injury. Methods: Seven adult horses. Methods: After induction of general anesthesia and ventral median celiotomy, ischemia was created by arteriovenous (AVO) and lumen occlusion of a 20-cm segment of jejunum and ascending colon for 70 minutes, followed by a 60-minute reperfusion period. Intraluminal distention (25 cm H2O) was created in a second 20-cm jejunal segment and maintained within the abdomen for 120 minutes, followed by a 120-minute decompression period. Seromuscular biopsies were obtained upon entering the abdomen and after the ischemic and reperfusion periods, and after the distention and decompression periods along with corresponding control seromuscular biopsies. Samples were processed and examined by light microscopy, transmission electron, and scanning electron microscopy. Results: Ischemia and reperfusion, and intraluminal distention and decompression, resulted in severe morphologic changes in the seromuscular layer of equine jejunum. A similar period of ischemia-reperfusion caused minimal changes in the ascending colon serosa. Conclusions: Adult equine jejunum sustains more serosal damage than the ascending colon after similar periods of ischemia-reperfusion injury. Intraluminal distention and subsequent decompression causes serosal damage in the equine jejunum. Conclusions: The small intestine is more susceptible to seromuscular layer damage than the ascending colon.
Publication Date: PubMed ID: 11230765 The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
Summary
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The study investigates the changes that occur in the intestinal tissue of horses following artificial induction of ischemia-reperfusion or intraluminal distention-decompression, focusing on two areas: the jejunum and the ascending colon. The research finds that the jejunum undergoes more damage compared to the ascending colon during these conditions.
Methodology
- Seven adult horses were subjected to the experiment.
- General anesthesia and a surgical procedure called ventral median celiotomy were performed on the animals.
- Ischemia (restriction in blood supply) was brought about in a 20-cm segment of jejunum and ascending colon through arteriovenous (AVO) and lumen occlusion for 70 minutes, followed by reperfusion (restoration of blood supply) for 60 minutes.
- Intraluminal distention (internal expansion) was created in a second 20-cm jejunal segment using a pressure of 25 cm H2O and was maintained inside the abdomen for 120 minutes, succeeded by decompression for 120 minutes.
- Biopsy samples were taken from the seromuscular layer of the intestines at the start of the experiment, after the ischemia and reperfusion stages, and after the distention and decompression stages.
- Microscope inspections were done using light microscopy, transmission electron microscopy, and scanning electron microscopy.
Results
- Ischemia-reperfusion and intraluminal distention-decompression caused considerable morphological changes to the jejunal seromuscular layer.
- Despite the impairment seen in the jejunum, the ascending colon showed minimal alterations after being subjected to the same period of ischemia and reperfusion.
- The findings suggest that the small intestine (jejunum) is more prone to damage than the ascending colon (part of the large intestine).
Conclusion
- When the conditions of ischemia-reperfusion and intraluminal distention-decompression were experimentally induced, the tissues of the equine jejunum sustained more damage compared to the ascending colon.
- The results of this study contribute to an improved understanding of gastroenterological health in horses. This could influence veterinary practices, specifically those related to treatment strategies for intestinal conditions in these animals.
Cite This Article
APA
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Serosal injury in the equine jejunum and ascending colon after ischemia-reperfusion or intraluminal distention and decompression.
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Publication
Researcher Affiliations
Citations
This article has been cited 8 times.- Bardell D, Rocchigiani G, Ressel L, Milner P. Histological Evaluation of Resected Tissue as a Predictor of Survival in Horses with Strangulating Small Intestinal Disease. Animals (Basel) 2023 Aug 26;13(17).
- Hou J, Ness SS, Tschudi J, O'Farrell M, Veddegjerde R, Martinsen ØG, Tønnessen TI, Strand-Amundsen R. Assessment of Intestinal Ischemia-Reperfusion Injury Using Diffuse Reflectance VIS-NIR Spectroscopy and Histology. Sensors (Basel) 2022 Nov 24;22(23).
- Grages AM, Verhaar N, Pfarrer C, Breves G, Burmester M, Neudeck S, Kästner S. Low Flow versus No Flow: Ischaemia Reperfusion Injury Following Different Experimental Models in the Equine Small Intestine. Animals (Basel) 2022 Aug 22;12(16).
- Hou J, Strand-Amundsen R, Hødnebø S, Tønnessen TI, Høgetveit JO. Assessing Ischemic Injury in Human Intestine Ex Vivo with Electrical Impedance Spectroscopy. J Electr Bioimpedance 2021 Jan;12(1):82-88.
- Despoudi K, Mantzoros I, Ioannidis O, Loutzidou L, Christidis P, Chatzakis C, Gkasdaris G, Raptis D, Pramateftakis MG, Angelopoulos S, Zaraboukas T, Koliakos G, Tsalis K. Healing of colonic anastomosis in rats under obstructive ileus conditions. Discoveries (Craiova) 2021 Apr-Jun;9(2):e129.
- Strand-Amundsen RJ, Reims HM, Reinholt FP, Ruud TE, Yang R, Høgetveit JO, Tønnessen TI. Ischemia/reperfusion injury in porcine intestine - Viability assessment. World J Gastroenterol 2018 May 14;24(18):2009-2023.
- Despoudi K, Mantzoros I, Ioannidis O, Cheva A, Antoniou N, Konstantaras D, Symeonidis S, Pramateftakis MG, Kotidis E, Angelopoulos S, Tsalis K. Effects of albumin/glutaraldehyde glue on healing of colonic anastomosis in rats. World J Gastroenterol 2017 Aug 21;23(31):5680-5691.
- Faleiros RR, Macoris DG, Alves GE, Souza DG, Teixeira MM, Moore RM. Local and remote lesions in horses subjected to small colon distension and decompression. Can J Vet Res 2008 Jan;72(1):68-76.
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