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Home / Equine Research Bank / Vet Immunol Immunopathol / Serum amyloid A isoforms in serum and synovial fluid in hors...
Veterinary immunology and immunopathology2005; 110(3-4); 325-330; doi: 10.1016/j.vetimm.2005.10.012

Serum amyloid A isoforms in serum and synovial fluid in horses with lipopolysaccharide-induced arthritis.

Abstract: The aim of the study was to determine the intraarticular serum amyloid A (SAA) response pattern in horses with inflammatory arthritis. Inflammatory arthritis was induced by injection of lipopolysaccharide (LPS) into the radiocarpal joint of four horses. Serum and synovial fluid (SF) samples were collected before and at 4, 8, 12, 24, 48, 72, 96, and 144 h after injection. Concentrations of SAA were measured by immunoturbidometry, and expression of SAA isoforms was visualized by denaturing isoelectric focusing and Western blotting. The LPS injection caused systemic and local clinical signs of inflammation. Serum amyloid A appeared in serum and SF within 8h after LPS injection. Isoelectric focusing showed three major SAA bands with apparent isoelectric points (pI) of 7.9, 8.6, and >9.3 in serum and SF. Synovial fluid contained two additional isoforms with highly alkaline apparent pI values (apparent pI value extrapolated from standard curve=10.0 and 10.2), which were not present in any of the serum samples. In conclusion, intraarticular injection of LPS induced systemic and local inflammatory responses in the horses. By demonstrating SF-specific SAA isoforms the results of the present study suggest that SAA is synthesized locally in the equine inflamed joint, similar to what has been demonstrated in humans previously. The marked local SAA synthesis suggests an important pathophysiological role in inflammatory arthritis.
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Publication Date: 2005-12-05 PubMed ID: 16337010DOI: 10.1016/j.vetimm.2005.10.012Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to observe the patterns of serum amyloid A (SAA), a critical biomarker for inflammation, in horses with inflammatory arthritis triggered by the injection of lipopolysaccharide (LPS). The study found that LPS injection caused systemic and local inflammation, with SAA appearing in the serum and synovial fluid within 8 hours of injection. Notably, the synovial fluid showed two additional SAA isoforms, implying that SAA is locally synthesized in the inflamed joint, a phenomenon observed in human studies as well.

Objective and Methodology

  • The primary objective of the study was to comprehend the intraarticular response pattern of Serum amyloid A (SAA) in horses affected by inflammatory arthritis.
  • Inflammatory arthritis was artificially induced in four horses using an injection of lipopolysaccharide (LPS) into a specific joint (radiocarpal joint).
  • Samples of synovial fluid (SF) and serum were collected at set intervals from before the injection up to 144 hours after the injection.
  • The concentrations of SAA in the samples were determined through a process called immunoturbidometry while the different forms of SAA were visualized using isoelectric focusing and Western blotting techniques.

Results and Findings

  • The injection of LPS resulted in both local and systemic signs of inflammation.
  • Following the LPS injection, SAA was observed in both the serum and SF within 8 hours.
  • The isoelectric focusing revealed three main SAA bands in serum and SF with apparent isoelectric points of 7.9, 8.6, and >9.3.
  • Noticeably, two additional isoforms of SAA with highly alkaline apparent pI values (10.0 and 10.2) were only found in the synovial fluid, not in the serum samples.

Conclusions

  • The study established that injecting LPS into a joint causes both local and systemic inflammatory responses in horses.
  • The presence of two additional SAA isoforms solely in the synovial fluid implies that similar to observations in humans, SAA could be locally synthesized in the inflamed joints of horses.
  • These findings indicate a potentially significant role of local SAA synthesis in the pathophysiology of inflammatory arthritis, although further study is required to fully understand the implications.

Cite This Article

APA
Jacobsen S, Niewold TA, Halling-Thomsen M, Nanni S, Olsen E, Lindegaard C, Andersen PH. (2005). Serum amyloid A isoforms in serum and synovial fluid in horses with lipopolysaccharide-induced arthritis. Vet Immunol Immunopathol, 110(3-4), 325-330. https://doi.org/10.1016/j.vetimm.2005.10.012

Publication

Veterinary immunology and immunopathology
ISSN: 0165-2427
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 110
Issue: 3-4
Pages: 325-330

Researcher Affiliations

Jacobsen, Stine
  • Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlaegevej 48, DK-1870 Frederiksberg C, Copenhagen, Denmark. stj@kvl.dk
Niewold, Theo A
    Halling-Thomsen, Maj
      Nanni, Simone
        Olsen, Emil
          Lindegaard, Casper
            Andersen, Pia Haubro

              MeSH Terms

              • Animals
              • Arthritis / blood
              • Arthritis / chemically induced
              • Arthritis / metabolism
              • Arthritis / veterinary
              • Female
              • Forelimb
              • Horse Diseases / blood
              • Horse Diseases / chemically induced
              • Horse Diseases / metabolism
              • Horses
              • Leukocyte Count
              • Lipopolysaccharides / pharmacology
              • Male
              • Protein Isoforms / analysis
              • Protein Isoforms / blood
              • Serum Amyloid A Protein / analysis
              • Synovial Fluid / chemistry
              • Synovial Fluid / cytology
              • Time Factors

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