Signal Transduction of Eel Luteinizing Hormone Receptor (eelLHR) and Follicle Stimulating Hormone Receptor (eelFSHR) by Recombinant Equine Chorionic Gonadotropin (rec-eCG) and Native eCG.
Abstract: Previous studies showed that recombinant equine chorionic gonadotropin (rec-eCGβ/α) exhibits both follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells. In this study, we analyzed signal transduction by eelFSHR and eelLHR upon stimulation with rec-eCGβ/α and native eCG. The cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR was determined upon exposure to different doses (0-1,450 ng/mL) of rec-eCGβ/α and native eCG. The EC values of rec-eCGβ/α and native eCG were 172.4 and 786.6 ng/mL, respectively. The activity of rec-eCGβ/α was higher than that of native eCG. However, signal transduction in the CHO PathHunter Parental cells expressing eelFSHR was not enhanced by stimulation with both agonist rec-eCGβ/α and native eCG. We concluded that rec-eCGβ/α and native eCG were completely active in cells expressing eelLHR, similar to the activity in the mammalian cells expressing LHRs. However, rec-eCGβ/α and native eCG did not invoke any signaling response in the cells expressing eelFSHR. These results suggest that eCG has a potent activity in cells expressing eelLHR. Thus, we also suggest that rec-eCGβ/α can induce eel maturation by administering gonadotropic reagents (LH), such as salmon pituitary extract.
Publication Date: 2018-03-31 PubMed ID: 29707684PubMed Central: PMC5915767DOI: 10.12717/DR.2018.22.1.055Google Scholar: Lookup
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Summary
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The research studied signal transmission in eel luteinizing hormone receptors (eelLHR) and follicle-stimulating hormone receptors (eelFSHR) exposed to recombinant equine chorionic gonadotropin (rec-eCGβ/α) and native eCG. The recombinant was more active than native eCG in eelLHR-expressing cells, but neither triggered a response in eelFSHR-expressing cells. The findings suggest eCG and its recombinant form could induce maturation in eels via treatment with luteinizing hormone-based agents.
Exploring Eel Hormone Receptors
- The paper presents research into how rec-eCGβ/α and native eCG affect eel luteinizing hormone receptors (eelLHR) and follicle-stimulating hormone receptors (eelFSHR).
- Prior studies demonstrated that rec-eCGβ/α has follicle-stimulating hormone (FSH) and luteinizing hormone (LH)-like activities in rat LHR- and FSHR-expressing cells.
Signal Transduction in EelLHR Cells
- The researchers measured the cyclic adenosine monophosphate (cAMP) stimulation in CHO-K1 cells expressing eelLHR when exposed to varying doses of rec-eCGβ/α and native eCG.
- The EC values of rec-eCGβ/α and native eCG were 172.4 and 786.6 ng/mL, respectively.
- What this indicates is that rec-eCGβ/α was more active than the native eCG.
- This implies that the recombinant variant is more effective in inducing a response in eelLHR.
Lack of Response in EelFSHR Cells
- In contrast, signal transmission in the CHO PathHunter Parental cells expressing eelFSHR was not enhanced by stimulation with both rec-eCGβ/α and native eCG.
- This means that neither rec-eCGβ/α nor native eCG triggered any signaling response in the cells expressing eelFSHR.
- Therefore, these hormones appear to have no effect on the follicle-stimulating hormone receptors in eels.
Implications of the Study
- The research shows that rec-eCGβ/α and native eCG were completely active in cells expressing eelLHR, similar to activity in mammalian cells expressing LHRs.
- These results suggest that eCG has potent activity in cells expressing eelLHR.
- Thus, rec-eCGβ/α could potentially be used to induce maturation in eels by administering gonadotropic reagents (LH), such as salmon pituitary extract.
- This research adds to our understanding of eel reproduction and may present a new means to stimulate eel maturation through hormonal interventions.
Cite This Article
APA
Byambaragchaa M, Lee SY, Kim DJ, Kang MH, Min KS.
(2018).
Signal Transduction of Eel Luteinizing Hormone Receptor (eelLHR) and Follicle Stimulating Hormone Receptor (eelFSHR) by Recombinant Equine Chorionic Gonadotropin (rec-eCG) and Native eCG.
Dev Reprod, 22(1), 55-64.
https://doi.org/10.12717/DR.2018.22.1.055 Publication
Researcher Affiliations
- Animal Biotechnology, Graduate School of Future Convergence Technology, Dept. of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Korea.
- Animal Biotechnology, Graduate School of Future Convergence Technology, Dept. of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Korea.
- Aquaculture Research Division, National Institute of Fisher Science (NIFS), Busan 46083, Korea.
- Dept. of Food Science and Nutrition, Hoseo University, Asan 31499, Korea.
- Animal Biotechnology, Graduate School of Future Convergence Technology, Dept. of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Ansung 17579, Korea.
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Citations
This article has been cited 5 times.- Byambaragchaa M, Joo HE, Kim SG, Kim YJ, Park GE, Min KS. Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR). Dev Reprod 2022 Mar;26(1):1-12.
- Byambaragchaa M, Choi SH, Kim DW, Min KS. Cell-Surface Loss of Constitutive Activating and Inactivating Mutants of Eel Luteinizing Hormone Receptors. Dev Reprod 2021 Dec;25(4):225-234.
- Santos-Jimenez Z, Meza-Herrera CA, Calderon-Leyva G, Martinez-Ros P, Guillen-Muñoz JM, Gonzalez-Bulnes A. Efficiency of hCG for Inducing Resumption of Ovarian Cyclicity and Synchronized Ovulations during the Seasonal Anestrous in Sheep. Animals (Basel) 2021 Nov 5;11(11).
- Min KS, Park JJ, Lee SY, Byambaragchaa M, Kang MH. Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG). BMC Biotechnol 2020 Nov 11;20(1):59.
- Kim JM, Byambaragchaa M, Kang MH, Min KS. The C-terminal Phosphorylation Sites of eel Follicle-Stimulating Hormone Receptor are Important Role in the Signal Transduction. Dev Reprod 2018 Jun;22(2):143-153.
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