Similarities and differences in supporting and chromaffin cells in the mammalian adrenal medullae: an immunohistochemical study.
Abstract: The adrenal medulla is a typical paraganglion, having the same origin as the sympathetic ganglia, and contains at least two types of parenchymal cells: chromaffin cells and supporting cells. We previously reported that the extent of cellular association of chromaffin cells with supporting cells was remarkably higher in noradrenaline (NA)-than in adrenaline (A)-cell regions in the adrenal medullae of the rat and pig. Methods: Cryostat sections of adrenal medullae of nine mammalian species fixed with Zamboni fluid for 24 h were immunostained by ABC methods using antisera to S-100 protein and PNMT. Results: The distribution patterns of A and NA cells in the adrenal medullae were classified into four types. In the chipmunk and rabbit, adrenomedullary chromaffin cells consisted of A cells. S-100-immunoreactive cells were present more frequently in NA- than in A-cell regions in seven species (rat, golden hamster, cat, dog, pig, ox, and horse). These cells sent out cytoplasmic processes and formed a network by immunoreactive elements among NA cells. The cell-association patterns of S-100-positive cells with NA cell were classified into three types. In A-cell regions, only a few S-100-positive cells were seen in most of the species, although the frequency of S-100-labeled cells were exceptionally high in the horse. Conclusions: The close association of supporting cells with NA cells was commonly found in the adrenal medulla in many mammalian species, irrespective of the proportions and distribution patterns of A cells and NA cells. On the other hand, species differences existed in details of the cellular association between supporting cells and NA cells.
Publication Date: 1996-03-01 PubMed ID: 8742700DOI: 10.1002/(SICI)1097-0185(199603)244:3<358::AID-AR7>3.0.CO;2-UGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study explores the similarities and differences in supporting and chromaffin cells in the adrenal medullae of different mammalian species. The findings indicate that these cells show distinct levels of association depending on whether they are located in noradrenaline (NA) or adrenaline (A) cell regions, with a significant level of variation between species.
Research Methods
- The research used adrenal medullae specimens from nine different mammalian species which were preserved using a substance called Zamboni fluid.
- The preserved samples were then cut into thin slices using a tool called a cryostat for detailed examination.
- The researchers employed an immunostaining technique known as the ABC method, along with antisera to S-100 protein and PNMT, to highlight and differentiate between various types of cells.
Research Findings
- The distribution of adrenaline (A) and noradrenaline (NA) cells in the adrenal medullae showed four distinct patterns across the species examined.
- In two of the species, the chipmunk and the rabbit, the adrenomedullary chromaffin cells were all A cells.
- In seven out of nine species (namely, the rat, golden hamster, cat, dog, pig, ox, and horse), S-100-positive cells were found more frequently in noradrenaline (NA) areas than in adrenaline (A) areas.
- The researchers noticed that these S-100-positive cells extended cytoplasmic processes and formed a network amidst the NA cells.
- The study found that the number of S-100-positive cells in adrenaline areas was comparatively lower. The exception to this was observed in horses, where the prevalence of S-100-positive cells in adrenaline areas was unexpectedly high.
Conclusions
- The research found a common trend across many mammalian species, where supporting cells were closely associated with noradrenaline (NA) cells in the adrenal medulla.
- However, the details of this cellular association between supporting cells and noradrenaline cells varied significantly among species, underscoring the importance of individual species characteristics in adrenal medulla composition and function.
Cite This Article
APA
Suzuki T, Kachi T.
(1996).
Similarities and differences in supporting and chromaffin cells in the mammalian adrenal medullae: an immunohistochemical study.
Anat Rec, 244(3), 358-365.
https://doi.org/10.1002/(SICI)1097-0185(199603)244:3<358::AID-AR7>3.0.CO;2-U Publication
Researcher Affiliations
- Department of Anatomy, Hirosaki University School of Medicine, Japan.
MeSH Terms
- Adrenal Medulla / chemistry
- Adrenal Medulla / cytology
- Animals
- Cats
- Chromaffin System / chemistry
- Chromaffin System / cytology
- Cricetinae
- Dogs
- Epinephrine
- Horses
- Immunohistochemistry
- Mammals / anatomy & histology
- Norepinephrine
- Rabbits
- Rats
- Rats, Wistar
- S100 Proteins / analysis
- Sciuridae
- Species Specificity
Citations
This article has been cited 5 times.- Kawamoto B, Shimizu S, Shimizu T, Higashi Y, Honda M, Sejima T, Saito M, Takenaka A. Angiotensin II centrally induces frequent detrusor contractility of the bladder by acting on brain angiotensin II type 1 receptors in rats.. Sci Rep 2016 Feb 24;6:22213.
- Nakamura K, Shimizu T, Yanagita T, Nemoto T, Taniuchi K, Shimizu S, Dimitriadis F, Yawata T, Higashi Y, Ueba T, Saito M. Angiotensin II acting on brain AT1 receptors induces adrenaline secretion and pressor responses in the rat.. Sci Rep 2014 Nov 28;4:7248.
- Manier ML, Spraggins JM, Reyzer ML, Norris JL, Caprioli RM. A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry.. J Mass Spectrom 2014 Aug;49(8):665-73.
- Wu C, Dill AL, Eberlin LS, Cooks RG, Ifa DR. Mass spectrometry imaging under ambient conditions.. Mass Spectrom Rev 2013 May-Jun;32(3):218-43.
- Huynh TT, Pacak K, Brouwers FM, Abu-Asab MS, Worrell RA, Walther MM, Elkahloun AG, Goldstein DS, Cleary S, Eisenhofer G. Different expression of catecholamine transporters in phaeochromocytomas from patients with von Hippel-Lindau syndrome and multiple endocrine neoplasia type 2.. Eur J Endocrinol 2005 Oct;153(4):551-63.
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