Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure.
Abstract: For many years in human assisted-reproduction procedures there have been special protocols to prepare and improve sperm quality. Colloidal centrifugation (CC) is a useful technique that has been proved to enhance semen quality by selection of the best spermatozoa for different species. Its use is recommended to improve fertility of subfertile stallions but current CC protocols are clinically complicated in the equine sperm processing technique due to economic and technical difficulties. The aim of this study was to determine the optimal processing procedures to adapt the use of a CC product (EquiPure™) in the equine reproduction industry. A total of nineteen ejaculates were collected from 10 Purebred Spanish Horses (P.R.E horses) using a Missouri artificial vagina. Gel-free semen aliquots were analyzed prior to treatment (control). Semen was subjected to one of six CC protocols with EquiPure™ and centrifuged samples were statistically evaluated by ANOVA and Duncan tests (p<0.05) for sperm quality and recovery rate. We obtained higher values by colloidal centrifugation in LIN, STR and BCF variables and DNA fragmentation index trended to be lower in most of the CC protocols. The studied protocols were shown to be as efficient in improving equine sperm quality as the current commercial EquiPure™, with the added advantage of being much more economical and simple to use. According to these results it seems to be possible to incorporate single layer and or high colloidal centrifugation volume protocols what would make them simple, economic and clinically viable for the equine sperm processing procedure.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-02-16 PubMed ID: 21367542DOI: 10.1016/j.anireprosci.2011.02.001Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study explores the use and optimization of colloidal centrifugation protocols to improve equine sperm quality, an effort to make these methods cost-effective and technically less complicated.
Research Objective and Background
- The aim of this research is to improve and adapt the use of colloidal centrifugation protocols for equine sperm processing, particularly with a product named EquiPure™. The goal is to boost sperm quality, making it economically viable and simple to apply.
- Colloidal centrifugation (CC) is a method extensively used in assisted-reproduction procedures to enhance sperm quality. It works by selecting the best spermatozoa for a range of species. While it’s proven to enhance semen quality significantly, integrating it into equine sperm processing can be challenging due to economic and technical barriers.
Methodology
- 19 ejaculates were collected from 10 Purebred Spanish Horses (P.R.E horses) using a Missouri artificial vagina. The semen samples were analyzed prior to treatment to provide a control group.
- The samples were then subjected to one of six CC protocols with EquiPure™, focusing on sperm quality and recovery rate. Statistical evaluation was performed using ANOVA and Duncan tests.
Results
- Colloidal centrifugation resulted in improved values in LIN, STR, and BCF variables, indicating better sperm quality. DNA fragmentation index tended to be lower in most of the CC protocols, suggesting less damage to sperm DNA.
- The tested protocols proved to be as efficient as the commercially available EquiPure™ in enhancing equine sperm quality. Notably, the adapted methods were much more economical and straightforward, showing the potential to make CC a viable option for equine sperm processing.
Conclusion
- In summary, the research indicates that it’s feasible to incorporate single layer and high colloidal centrifugation volume protocols into the equine sperm processing procedure. This is due to their efficiency in improving sperm quality, affordability, and simpler application in comparison to traditional methods.
Cite This Article
APA
Gutiérrez-Cepeda L, Fernández A, Crespo F, Gosálvez J, Serres C.
(2011).
Simple and economic colloidal centrifugation protocols may be incorporated into the clinical equine sperm processing procedure.
Anim Reprod Sci, 124(1-2), 85-89.
https://doi.org/10.1016/j.anireprosci.2011.02.001 Publication
Researcher Affiliations
- Animal Medicine and Surgery Department, Veterinary Faculty, UCM, Avda. Puerta de Hierro s/n, Ciudad Universitaria, 28040 Madrid, Spain. luna.gutierrez@vet.ucm.es
MeSH Terms
- Animals
- Centrifugation / economics
- Centrifugation / methods
- Centrifugation / veterinary
- Clinical Protocols
- Horses
- Male
- Semen Analysis / methods
- Semen Analysis / veterinary
- Specimen Handling / methods
- Specimen Handling / veterinary
- Sperm Retrieval / veterinary
Citations
This article has been cited 4 times.- Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation. Animals (Basel) 2023 Jan 22;13(3).
- Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA. Acta Vet Scand 2012 Dec 5;54(1):72.
- Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Pérez-Aguilera V, Oliet A, Hijón V, Moreno S, González-Zorn B, Gutiérrez-Cepeda L. Innovative Approaches to Avoid Antibiotic Use in Equine Semen Cryopreservation: Advancing Sustainable Reproductive Technologies. Animals (Basel) 2025 May 9;15(10).
- Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Pérez-Aguilera V, Galán C, Domínguez-Gimbernat M, Oliet A, Moreno S, González-Zorn B, Gutiérrez-Cepeda L. Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen. Animals (Basel) 2024 Jan 5;14(2).
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