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Home / Equine Research Bank / Toxins (Basel) / Simultaneous Determination of Pyrethrins, Pyrethroids, and P...
Toxins2023; 15(6); 401; doi: 10.3390/toxins15060401

Simultaneous Determination of Pyrethrins, Pyrethroids, and Piperonyl Butoxide in Animal Feeds by Liquid Chromatography-Tandem Mass Spectrometry.

Abstract: The presence of insecticides like pyrethrins and synthetic pyrethroids, combined with the synergist piperonyl butoxide, in animal feeds can pose a risk to both animal and human health by contaminating the food chain. In this study, a simple and fast method was developed for the simultaneous determination of these compounds in contaminated animal feeds using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Sample preparation was carried out using a QuEChERS-based approach, and the method was validated with acceptable accuracy ranging from 84 to 115% and precision below 10%. The limit of detection (LOD) and limit of quantification (LOQ) were between 0.15 and 3 and 1 and 10 µg/kg, respectively. The method detected insecticide contaminations in various livestock and poultry feeds. Furthermore, the method was applied to a toxicology case, where it identified and quantified piperonyl butoxide and deltamethrin in the submitted horse feed sample. These results demonstrate that the method can be a valuable tool in animal health and food safety diagnostic applications, as well as veterinary toxicology investigations concerning pyrethrin-related feed contamination.
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Publication Date: 2023-06-17 PubMed ID: 37368701PubMed Central: PMC10305105DOI: 10.3390/toxins15060401Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focuses on the development of a fast and simple technique to simultaneously detect the presence of insecticides, particularly pyrethrins and synthetic pyrethroids, along with piperonyl butoxide in animal feeds. The researchers used liquid chromatography-tandem mass spectrometry to validate the method and manage to identify these substances in various livestock feeds.

Method Development and Validation

  • The method used for this research combined liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a QuEChERS-based approach to sample preparation. This technique allows for the fast simultaneous detection of pyrethrins, synthetic pyrethroids and piperonyl butoxide in animal feeds.
  • The researchers validated the method and reported its accuracy to range from 84 to 115%, showing a good level of precision as being below 10%. This means that the method is reliable and could repeatedly produce consistent results.

Detection and Quantification

  • The lower limit of detection (LOD) and limit of quantification (LOQ) were found to be between 0.15 and 3 and 1 and 10 µg/kg, respectively. These numbers signify the smallest amount of the substances that could be reliably detected and quantified by the method, which are quite low amounts, affirming the sensitivity of the method.
  • The method was then used to detect insecticide contaminations in various livestock and poultry feeds. The successful detection in different feed types shows that the method is versatile and useful for a broad range of animal feeds.

Application to Veterinary Toxicology

  • The newly-developed method was also applied to a real-world toxicology case to detect the presence of piperonyl butoxide and deltamethrin in a horse feed sample. This illustrates that the method can be useful not only in laboratory settings but also in practical cases for identifying harmful substances.
  • The findings of this study contribute to safeguarding both animal and human health, as the contamination of animal feeds can indirectly affect humans via the food chain. The researchers suggest that this method would be valuable for animal health and food safety diagnostics, and especially useful for veterinary toxicology investigations related to feed contamination.

Cite This Article

APA
Xu X, Murphy LA. (2023). Simultaneous Determination of Pyrethrins, Pyrethroids, and Piperonyl Butoxide in Animal Feeds by Liquid Chromatography-Tandem Mass Spectrometry. Toxins (Basel), 15(6), 401. https://doi.org/10.3390/toxins15060401

Publication

Toxins
ISSN: 2072-6651
NlmUniqueID: 101530765
Country: Switzerland
Language: English
Volume: 15
Issue: 6
PII: 401

Researcher Affiliations

Xu, Xin
  • Pennsylvania Animal Diagnostic Laboratory System Toxicology Laboratory, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, 382 W Street Rd., Kennett Square, PA 19348, USA.
Murphy, Lisa A
  • Pennsylvania Animal Diagnostic Laboratory System Toxicology Laboratory, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, 382 W Street Rd., Kennett Square, PA 19348, USA.

MeSH Terms

  • Humans
  • Animals
  • Horses
  • Pyrethrins / analysis
  • Piperonyl Butoxide / analysis
  • Chromatography, Liquid / methods
  • Tandem Mass Spectrometry / methods
  • Insecticides
  • Animal Feed / analysis

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 30 references
  1. Anadón A, Martínez-Larrañaga MR, Martínez MA. Use and abuse of pyrethrins and synthetic pyrethroids in veterinary medicine.. Vet J 2009 Oct;182(1):7-20.
    doi: 10.1016/j.tvjl.2008.04.008pubmed: 18539058google scholar: lookup
  2. Ravula AR, Yenugu S. Pyrethroid based pesticides - chemical and biological aspects.. Crit Rev Toxicol 2021 Feb;51(2):117-140.
    doi: 10.1080/10408444.2021.1879007pubmed: 33821762google scholar: lookup
  3. Anadón A, Arés I, Martínez M.A, Martínez-Larrañaga M.R. Pyrethrins and Synthetic Pyrethroids: Use in Veterinary Medicine. In: Ramawat K.G., Mérillon J., editors. Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes. Springer; Berlin/Heidelberg, Germany: 2013. pp. 4061–4086.
  4. Ensley S.M. Chapter 39–Pyrethrins and Pyrethroids. In: Gupta R.C., editor. Veterinary Toxicology. 3rd ed. Academic Press; Cambridge, MA, USA: 2018. pp. 515–520.
  5. Davies TG, Field LM, Usherwood PN, Williamson MS. DDT, pyrethrins, pyrethroids and insect sodium channels.. IUBMB Life 2007 Mar;59(3):151-62.
    doi: 10.1080/15216540701352042pubmed: 17487686google scholar: lookup
  6. Feyereisen R. Insect P450 inhibitors and insecticides: challenges and opportunities.. Pest Manag Sci 2015 Jun;71(6):793-800.
    doi: 10.1002/ps.3895pubmed: 25404103google scholar: lookup
  7. Damalas CA, Eleftherohorinos IG. Pesticide exposure, safety issues, and risk assessment indicators.. Int J Environ Res Public Health 2011 May;8(5):1402-19.
    doi: 10.3390/ijerph8051402pmc: PMC3108117pubmed: 21655127google scholar: lookup
  8. Ahamad A, Kumar J. Pyrethroid Pesticides: An Overview on Classification, Toxicological Assessment and Monitoring. J. Hazard. Mater. Adv. 2023;10:100284.
    doi: 10.1016/j.hazadv.2023.100284google scholar: lookup
  9. Chrustek A, Hołyńska-Iwan I, Dziembowska I, Bogusiewicz J, Wróblewski M, Cwynar A, Olszewska-Słonina D. Current Research on the Safety of Pyrethroids Used as Insecticides.. Medicina (Kaunas) 2018 Aug 28;54(4).
    doi: 10.3390/medicina54040061pmc: PMC6174339pubmed: 30344292google scholar: lookup
  10. Hołyńska-Iwan I, Szewczyk-Golec K. Pyrethroids: How They Affect Human and Animal Health?. Medicina (Kaunas) 2020 Oct 30;56(11).
    doi: 10.3390/medicina56110582pmc: PMC7692614pubmed: 33143129google scholar: lookup
  11. Tuck S, Furey A, Crooks S, Danaher M. A review of methodology for the analysis of pyrethrin and pyrethroid residues in food of animal origin.. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2018 May;35(5):911-940.
    doi: 10.1080/19440049.2017.1420919pubmed: 29337656google scholar: lookup
  12. Oliveira LG, Kurz MHS, Guimarães MCM, Martins ML, Prestes OD, Zanella R, Ribeiro JNDS, Gonçalves FF. Development and validation of a method for the analysis of pyrethroid residues in fish using GC-MS.. Food Chem 2019 Nov 1;297:124944.
    doi: 10.1016/j.foodchem.2019.06.011pubmed: 31253283google scholar: lookup
  13. Jia F, Wang W, Wang J, Yin J, Liu Y, Liu Z. New Strategy to Enhance the Extraction Efficiency of Pyrethroid Pesticides in Fish Samples using a Modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) Method. Anal. Methods 2012;4:449–453.
    doi: 10.1039/c2ay05681jgoogle scholar: lookup
  14. Kim BJ, Yang SH, Choi H. Simultaneous Determination of Pyrethroid Insecticides in Foods of Animal Origins Using the Modified QuEChERS Method and Gas Chromatography-Mass Spectrometry.. Foods 2022 Nov 14;11(22).
    doi: 10.3390/foods11223634pmc: PMC9689788pubmed: 36429228google scholar: lookup
  15. Khay S, Abd El-Aty AM, Choi JH, Shin EH, Shin HC, Kim JS, Chang BJ, Lee CH, Shin SC, Jeong JY, Shim JH. Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC.. J Sep Sci 2009 Jan;32(2):244-51.
    doi: 10.1002/jssc.200800481pubmed: 19107766google scholar: lookup
  16. Stefanelli P, Santilio A, Cataldi L, Dommarco R. Multiresidue analysis of organochlorine and pyrethroid pesticides in ground beef meat by gas chromatography-mass spectrometry.. J Environ Sci Health B 2009 May;44(4):350-6.
    doi: 10.1080/03601230902801000pubmed: 19365750google scholar: lookup
  17. Barbini DA, Vanni F, Girolimetti S, Dommarco R. Development of an analytical method for the determination of the residues of four pyrethroids in meat by GC-ECD and confirmation by GC-MS.. Anal Bioanal Chem 2007 Nov;389(6):1791-8.
    doi: 10.1007/s00216-007-1440-7pubmed: 17680238google scholar: lookup
  18. Cheng J, Liu M, Yu Y, Wang X, Zhang H, Ding L, Jin H. Determination of pyrethroids in porcine tissues by matrix solid-phase dispersion extraction and high-performance liquid chromatography.. Meat Sci 2009 Aug;82(4):407-12.
    doi: 10.1016/j.meatsci.2008.09.011pubmed: 20416606google scholar: lookup
  19. Moloney M, Tuck S, Ramkumar A, Furey A, Danaher M. Determination of pyrethrin and pyrethroid residues in animal fat using liquid chromatography coupled to tandem mass spectrometry.. J Chromatogr B Analyt Technol Biomed Life Sci 2018 Mar 1;1077-1078:60-70.
    doi: 10.1016/j.jchromb.2017.12.022pubmed: 29413578google scholar: lookup
  20. Zheng W, Choi JM, Abd El-Aty AM, Yoo KH, Park DH, Kim SK, Kang YS, Hacımüftüoğlu A, Wang J, Shim JH, Shin HC. Simultaneous determination of spinosad, temephos, and piperonyl butoxide in animal-derived foods using LC-MS/MS.. Biomed Chromatogr 2019 Jun;33(6):e4493.
    doi: 10.1002/bmc.4493pubmed: 30663083google scholar: lookup
  21. Penagos-Tabares F, Sulyok M, Faas J, Krska R, Khiaosa-Ard R, Zebeli Q. Residues of pesticides and veterinary drugs in diets of dairy cattle from conventional and organic farms in Austria.. Environ Pollut 2023 Jan 1;316(Pt 2):120626.
    doi: 10.1016/j.envpol.2022.120626pubmed: 36370968google scholar: lookup
  22. US EPA. Tolerances and Exemptions for Pesticide Chemical Residues in Food. [(accessed on 20 March 2023)]; Available online: https://www.ecfr.gov/current/title-40/chapter-I/subchapter-E/part-180/subpart-C.
  23. Caloni F, Cortinovis C, Rivolta M, Davanzo F. Animal poisoning in Italy: 10 years of epidemiological data from the Poison Control Centre of Milan.. Vet Rec 2012 Apr 21;170(16):415.
    doi: 10.1136/vr.100210pubmed: 22271801google scholar: lookup
  24. Nagy A.L, Cortinovis C, Spicer L.J, Perego M.C, Caloni F. Long-Established and Emerging Pesticide Poisoning in Horses. Equine Vet. Educ. 2019;31:496–500.
    doi: 10.1111/eve.12887google scholar: lookup
  25. He FS, Deng H, Ji X, Zhang ZW, Sun JX, Yao PP. Changes of nerve excitability and urinary deltamethrin in sprayers.. Int Arch Occup Environ Health 1991;62(8):587-90.
    doi: 10.1007/BF00381112pubmed: 1856014google scholar: lookup
  26. Musarurwa H, Chimuka L, Pakade V.E, Tavengwa N.T. Recent Developments and Applications of QuEChERS Based Techniques on Food Samples during Pesticide Analysis. J. Food Compost. Anal. 2019;84:103314.
    doi: 10.1016/j.jfca.2019.103314google scholar: lookup
  27. US FDA Foods Program. Guidelines for the Validation of Chemical Methods for the Foods Program. [(accessed on 20 March 2023)]; Available online: https://www.fda.gov/food/laboratory-methods-food/foods-program-methods-validation-processes-and-guidelines.
  28. Kappenberg A, Juraschek LM. Development of a LC-MS/MS Method for the Simultaneous Determination of the Mycotoxins Deoxynivalenol (DON) and Zearalenone (ZEA) in Soil Matrix.. Toxins (Basel) 2021 Jul 7;13(7).
    doi: 10.3390/toxins13070470pmc: PMC8310301pubmed: 34357942google scholar: lookup
  29. Carballo D, Font G, Ferrer E, Berrada H. Evaluation of Mycotoxin Residues on Ready-to-Eat Food by Chromatographic Methods Coupled to Mass Spectrometry in Tandem.. Toxins (Basel) 2018 Jun 15;10(6).
    doi: 10.3390/toxins10060243pmc: PMC6024867pubmed: 29914055google scholar: lookup
  30. Maneeboon T, Chuaysrinule C, Mahakarnchanakul W. Optimization and Validation of Dispersive Liquid-Liquid Microextraction for Simultaneous Determination of Aflatoxins B1, B2, G1, and G2 in Senna Leaves and Pods Using HPLC-FLD with Pre-Column Derivatization.. Toxins (Basel) 2023 Apr 7;15(4).
    doi: 10.3390/toxins15040277pmc: PMC10142810pubmed: 37104215google scholar: lookup

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