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Nutrients2018; 10(12); 1871; doi: 10.3390/nu10121871

Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide.

Abstract: Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H₂O₂). ESCs were obtained from a young horse's skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H₂O₂ for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
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Publication Date: 2018-12-02 PubMed ID: 30513813PubMed Central: PMC6316332DOI: 10.3390/nu10121871Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a study that analyzed the effect of gamma-oryzanol (GO) on gene and miRNA expression, oxidative stress, and cell health in horse muscle cells pre-treated with hydrogen peroxide. The study found that GO modifies the expression of miRNA and genes, affecting processes like cell survival, damage, and oxidative stress.

Research Context

  • Gamma-oryzanol (GO) is a compound often used as a dietary supplement for performance animals like dogs and racehorses, as well as humans. Previous research has suggested that GO may influence oxidative stress reaction and post-exercise recovery in Thoroughbred horses.
  • The study aims to further understand the role of GO by analyzing its effects on miRNA (small non-coding RNA molecules that regulate gene expression) and gene expression, oxidative stress, and cell damage and longevity in equine satellite cells (ESCs), taken from a young horse’s skeletal muscle. These cells were pre-treated with GO and then exposed to hydrogen peroxide, a compound that induces oxidative stress.

Methodology

  • Microarray technique, a type of DNA sequencing, was used to assess the differences in miRNA and gene expression between control cells and those treated with GO.
  • Changes in expression were then validated using real time-quantitative polymerase chain reaction, a laboratory method used to amplify and simultaneously quantify a targeted DNA molecule.
  • Various tests related to cell longevity, damage, and oxidative stress were executed to better understand the broader effects of GO on the cells.

Findings

  • Microarray analysis showed a difference in 17 miRNA and 202 genes between the control and GO-treated equine satellite cells.
  • Assessments of cell longevity, damage, and oxidative stress demonstrated that GO has diverse effects on these processes, suggesting that GO can potentially mobilize the cell’s reaction to damage, enhance survival rate, and manage oxidative stress reactions.

Implications

  • The study’s findings suggest that GO supplementation can modify miRNA and gene expression in ESCs.
  • This alteration may affect processes such as tissue repair following injury, potentially influencing recovery rates in performance horses.
  • Understanding how dietary supplements like GO interact with gene and miRNA expression, oxidative stress, and cell recovery processes could help optimize animal performance and post-exercise recovery strategies.

Cite This Article

APA
Chodkowska KA, Ciecierska A, Majchrzak K, Ostaszewski P, Sadkowski T. (2018). Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide. Nutrients, 10(12), 1871. https://doi.org/10.3390/nu10121871

Publication

Nutrients
ISSN: 2072-6643
NlmUniqueID: 101521595
Country: Switzerland
Language: English
Volume: 10
Issue: 12
PII: 1871

Researcher Affiliations

Chodkowska, Karolina A
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. landadelka82@o2.pl.
Ciecierska, Anna
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. anna_ciecierska@sggw.pl.
Majchrzak, Kinga
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. kinga_majchrzak@sggw.pl.
Ostaszewski, Piotr
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. piotr_ostaszewski@sggw.pl.
Sadkowski, Tomasz
  • Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, Poland. tomasz_sadkowski@sggw.pl.

MeSH Terms

  • Animals
  • Apoptosis / drug effects
  • Cell Differentiation / drug effects
  • Cell Differentiation / genetics
  • Cell Proliferation / genetics
  • Cell Survival / drug effects
  • Cells, Cultured
  • Gene Expression / drug effects
  • Gene Expression Profiling / methods
  • Gene Expression Profiling / veterinary
  • Horses
  • Hydrogen Peroxide / pharmacology
  • Male
  • MicroRNAs / analysis
  • Oxidative Stress / drug effects
  • Phenylpropionates / pharmacology
  • RNA, Messenger / analysis
  • Satellite Cells, Skeletal Muscle / drug effects
  • Satellite Cells, Skeletal Muscle / physiology
  • Tissue Array Analysis / methods
  • Tissue Array Analysis / veterinary

Grant Funding

  • 2011/03/B/NZ5/05697 / Narodowe Centrum Nauki

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Citations

This article has been cited 1 times.
  1. Mowry KC, Thomson-Parker TL, Morales C, Fikes KK, Stutts KJ, Leatherwood JL, Anderson MJ, Smith RX, Suagee-Bedore JK. Effects of Crude Rice Bran Oil and a Flaxseed Oil Blend in Young Horses Engaged in a Training Program.. Animals (Basel) 2022 Nov 2;12(21).
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