Single nucleotide polymorphisms of myostatin gene in Chinese domestic horses.
Abstract: The myostatin gene (MSTN) is a genetic determinant of skeletal muscle growth. Single nucleotide polymorphisms (SNP) in MSTN are of importance due to their strong associations with horse racing performances. In this study, we screened the SNPs in MSTN gene in 514 horses from 15 Chinese horse breeds. Six SNPs (g.26T>C, g.156T>C, g.587A>G, g.598C>T, g.1485C>T, g.2115A>G) in MSTN gene were detected by sequencing and genotyped using PCR-RFLP method. The g.587A>G and g.598C>T residing in the 5'UTR region were novel SNPs identified by this study. The g.2115A>G which have previously been associated with racing performances were present in Chinese horse breeds, providing valuable genetic information for evaluating the potential racing performances in Chinese domestic breeds. The six SNPs together defined thirteen haplotypes, demonstrating abundant haplotype diversities in Chinese horses. Most of the haplotypes were shared among different breeds with no haplotype restricted to a specific region or a single horse breed. AMOVA analysis indicated that most of the genetic variance was attributable to differences among individuals without any significant contribution by the four geographical groups. This study will provide fundamental and instrumental genetic information for evaluating the potential racing performances of Chinese horse breeds.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication Date: 2013-12-22 PubMed ID: 24368331DOI: 10.1016/j.gene.2013.12.027Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study investigates the variations within the myostatin (MSTN) gene, associated with muscle growth and horse racing performances, in several Chinese horse breeds. The research identified six single nucleotide polymorphisms (SNPs), two of which are new, and showed the genetic diversity within Chinese horses, regardless of geographical location, providing valuable insights for evaluating racing potential.
Research Objectives and Methodology
- The primary objective of the study was to screen Single Nucleotide Polymorphisms (SNPs) in the myostatin (MSTN) gene, which is crucial because of its strong association with horse racing performances, among various Chinese horse breeds.
- A total of 514 horses from 15 different Chinese horse breeds were included in the study.
- The researchers detected the SNPs by sequencing and genetically typed them using a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
Findings of the Study
- The study identified six SNPs in the MSTN gene. These were designated as g.26T>C, g.156T>C, g.587A>G, g.598C>T, g.1485C>T, and g.2115A>G.
- Two of these SNPs (g.587A>G and g.598C>T), located in the 5’UTR region of the gene, were new SNPs that had not been previously identified.
- One SNP (g.2115A>G), which has been previously correlated with racing performances, was found in the Chinese horse breeds sampled. This finding provides valuable genetic information to assess the racing performance potential in these breeds.
- The six SNPs collectively defined thirteen haplotypes, indicating a considerable diversity of haplotypes within Chinese horse breeds. Significantly, no single haplotype was restricted to a specific region or single horse breed.
Significance and Implications of the Research
- The findings of this study shed light on the genetic diversity within various breeds of Chinese horses. This knowledge offers valuable insights into the potential racing performance of these breeds based on their specific genetic makeup.
- The identification of new SNPs broadens the understanding of the MSTN gene and its variations, providing additional avenues for further research.
- The study suggests that the observed genetic variance is mainly due to differences among individual horses rather than geographical groups. This finding could impact how breeders select and breed horses for optimal racing performance.
- This research provides foundational and instrumental genetic information that could be used in the evaluation and prediction of Chinese horse breeds’ potential racing performances.
Cite This Article
APA
Li R, Liu DH, Cao CN, Wang SQ, Dang RH, Lan XY, Chen H, Zhang T, Liu WJ, Lei CZ.
(2013).
Single nucleotide polymorphisms of myostatin gene in Chinese domestic horses.
Gene, 538(1), 150-154.
https://doi.org/10.1016/j.gene.2013.12.027 Publication
Researcher Affiliations
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
- Institute of Hexi Ecology and Oasis Agriculture, Hexi University, Zhangye, Gansu 734000, China.
- Animal Husbandry and Veterinary Bureau of Yining, Yili, Xinjiang 835100, China.
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
- Shaanxi University of Technology, Hanzhong, Shaanxi 723001, China.
- Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China.
- Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: leichuzhao1118@126.com.
MeSH Terms
- 5' Untranslated Regions
- Animals
- Animals, Inbred Strains
- China
- Horses / genetics
- Myostatin / genetics
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
Citations
This article has been cited 6 times.- Fan L, Zhu H, Wang K, Liu H, Hu W, Xu X, Yan S. Study on the Degradation of Methylene Blue by Cu-Doped SnSe.. Molecules 2023 Aug 10;28(16).
- Pira E, Vacca GM, Dettori ML, Piras G, Moro M, Paschino P, Pazzola M. Polymorphisms at Myostatin Gene (MSTN) and the Associations with Sport Performances in Anglo-Arabian Racehorses.. Animals (Basel) 2021 Mar 30;11(4).
- Padilha FGF, El-Jaick KB, de Castro L, Moreira ADS, Ferreira AMR. Effect of selection for eventing on the MSTN gene in Brazilian sport horses.. J Equine Sci 2018;29(1):21-24.
- Pokorska J, Kułaj D, Dusza M, Żychlińska-Buczek J, Makulska J. New Rapid Method of DNA Isolation from Milk Somatic Cells.. Anim Biotechnol 2016;27(2):113-7.
- Santagostino M, Khoriauli L, Gamba R, Bonuglia M, Klipstein O, Piras FM, Vella F, Russo A, Badiale C, Mazzagatti A, Raimondi E, Nergadze SG, Giulotto E. Genome-wide evolutionary and functional analysis of the Equine Repetitive Element 1: an insertion in the myostatin promoter affects gene expression.. BMC Genet 2015 Oct 26;16:126.
- Rosenbaum JT, Choi D, Wong A, Wilson DJ, Grossniklaus HE, Harrington CA, Dailey RA, Ng JD, Steele EA, Czyz CN, Foster JA, Tse D, Alabiad C, Dubovy S, Parekh PK, Harris GJ, Kazim M, Patel PJ, White VA, Dolman PJ, Edward DP, Alkatan HM, Al Hussain H, Selva D, Yeatts RP, Korn BS, Kikkawa DO, Stauffer P, Planck SR. The Role of the Immune Response in the Pathogenesis of Thyroid Eye Disease: A Reassessment.. PLoS One 2015;10(9):e0137654.
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