Sirius red is able to selectively stain eosinophil granulocytes in bovine, ovine and equine cervical tissue.
Abstract: The aim of the present study was to examine the suitability of sirius red staining for selective light microscopic demonstration of eosinophil granulocytes in cervical tissue of mares, cows and sheep. For this purpose, tissue was fixed in 4% neutral buffered formol or in Bouin's solution. Paraffin sections of 5-microm thickness were stained with sirius red. In cows, mares and sheep a selective distinction of eosinophilic infiltration is successful after both fixation methods.
Publication Date: 2004-05-18 PubMed ID: 15144288DOI: 10.1111/j.1439-0264.2004.00532.xGoogle Scholar: Lookup
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- Journal Article
Summary
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This research examines the efficacy of sirius red staining for highlighting the presence of eosinophil granulocytes in cervical tissue of cows, sheep and horses.
Objective of the Study
- The primary focus of this research was to investigate the effectiveness of sirius red as a stain for selectively demonstrating eosinophil granulocytes in the cervical tissue of cows, sheep, and mares (female horses) through light microscopic observation.
Methodology
- The research was conducted on tissues that were fixed using two primary methods. The tissues were fixed either in a 4% neutral buffered formol or in Bouin’s solution.
- Paraffin sections of the cervical tissue maintained at a thickness of about 5 micrometres were then stained using sirius red.
Findings
- The results of the study showed that, irrespective of the fixation method employed (whether 4% neutral buffered formol or Bouin’s solution), sirius red staining successfully enabled a selective distinction of eosinophilic infiltration in the cervical tissue of cows, mares, and sheep.
- This indicates that sirius red is effective in selectively staining eosinophil granulocytes in the cervical tissues of these three species, thereby providing a beneficial tool for future research studies relating to eosinophilic infiltration in such tissues.
Cite This Article
APA
Wehrend A, Hetzel U, Huchzermeyer S, Klein C, Bostedt H.
(2004).
Sirius red is able to selectively stain eosinophil granulocytes in bovine, ovine and equine cervical tissue.
Anat Histol Embryol, 33(3), 180-182.
https://doi.org/10.1111/j.1439-0264.2004.00532.x Publication
Researcher Affiliations
- Klinik für Geburtshilfe, Gynäkologie und Andrologie der Gross- und Kleintiere, Giessen, Germany. Azel.Wehrend@vetmed.uni-giessen.de
MeSH Terms
- Animals
- Azo Compounds
- Cattle
- Cervix Uteri / cytology
- Eosinophils / cytology
- Female
- Granulocytes / cytology
- Horses
- Sheep
- Staining and Labeling / methods
- Staining and Labeling / veterinary
Citations
This article has been cited 9 times.- Coutinho DS, Anjos-Valotta EA, do Nascimento CVMF, Pires ALA, Napimoga MH, Carvalho VF, Torres RC, E Silva PMR, Martins MA. 15-Deoxy-Delta-12,14-Prostaglandin J(2) Inhibits Lung Inflammation and Remodeling in Distinct Murine Models of Asthma. Front Immunol 2017;8:740.
- Biswas H, Longmore GD. Action of SNAIL1 in Cardiac Myofibroblasts Is Important for Cardiac Fibrosis following Hypoxic Injury. PLoS One 2016;11(10):e0162636.
- Chatterjee V, Gashev AA. Mast cell-directed recruitment of MHC class II positive cells and eosinophils towards mesenteric lymphatic vessels in adulthood and elderly. Lymphat Res Biol 2014 Mar;12(1):37-47.
- Guedes PT, de Oliveira BC, Manso PP, Caputo LF, Cotta-Pereira G, Pelajo-Machado M. Histological analyses demonstrate the temporary contribution of yolk sac, liver, and bone marrow to hematopoiesis during chicken development. PLoS One 2014;9(3):e90975.
- Van Meulder F, Van Coppernolle S, Borloo J, Rinaldi M, Li RW, Chiers K, Van den Broeck W, Vercruysse J, Claerebout E, Geldhof P. Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi. Infect Immun 2013 May;81(5):1798-809.
- Karaca T, Yörük M, Uslu S, Cetin Y, Uslu BA. Distribution of eosinophil granulocytes and mast cells in the reproductive tract of female goats in the preimplantation phase. Vet Res Commun 2009 Aug;33(6):545-54.
- Reineking W, Hennig-Pauka I, Schröder L, Höner U, Schreiber E, Geiping L, Lassnig S, Bonilla MC, Hewicker-Trautwein M, de Buhr N. Spontaneous Lethal Outbreak of Influenza A Virus Infection in Vaccinated Sows on Two Farms Suggesting the Occurrence of Vaccine-Associated Enhanced Respiratory Disease with Eosinophilic Lung Pathology. Viruses 2024 Jun 13;16(6).
- Benatar T, Cao MY, Lee Y, Lightfoot J, Feng N, Gu X, Lee V, Jin H, Wang M, Wright JA, Young AH. IL-17E, a proinflammatory cytokine, has antitumor efficacy against several tumor types in vivo. Cancer Immunol Immunother 2010 Jun;59(6):805-17.
- Benatar T, Cao MY, Lee Y, Li H, Feng N, Gu X, Lee V, Jin H, Wang M, Der S, Lightfoot J, Wright JA, Young AH. Virulizin induces production of IL-17E to enhance antitumor activity by recruitment of eosinophils into tumors. Cancer Immunol Immunother 2008 Dec;57(12):1757-69.
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