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Socket Preservation using Enzyme-treated Equine Bone Granules and an Equine Collagen Matrix: A Case Report with Histological and Histomorphometrical Assessment.
Abstract: To histologically assess the effectiveness of a socket-preservation technique using enzyme-treated equine bone granules as a bone-graft material in combination with an equine collagen matrix as a scaffold for soft-tissue regeneration. Background: Enzyme-treated equine bone granules and equine collagen matrix recently have been developed to help overcome alveolar bone deficiencies that develop in the wake of edentulism. Methods: The patient had one mandibular molar extracted and the socket grafted with equine bone granules. The graft was covered with the equine collagen matrix, placed in a double layer. No flap was prepared, and the gingival margins were stabilized with a single stitch, leaving the matrix partially exposed and the site to heal by secondary intention. The adjacent molar was extracted 1 month later, and that socket was left to heal by secondary intention without any further treatment. Three months after each surgery, an implant was placed and a biopsy was collected. The two biopsies underwent histological processing and qualitative evaluation. Histomorphometric analysis was also performed to calculate the percentage of newly formed bone (NFB) in the two cores. Healing at both sites was uneventful, and no inflammation or other adverse reactions were observed in the samples. Soft-tissue healing by secondary intention appeared to occur faster at the grafted site. The corresponding core showed a marked separation between soft and hard tissue that was not observed in the core from the nongrafted site, where soft-tissue hypertrophy could be observed. Newly formed bone at the grafted and nongrafted sites was not significantly different (27.2 ± 7.1 and 29.4 ± 6.2% respectively, p = 0.45). Conclusions: The surgical technique employed in this case appeared to facilitate postextraction soft-tissue healing by second intention and simplify soft-tissue management. Conclusions: Using a collagen-based matrix to cover a postextraction grafted site may facilitate second intention soft-tissue healing and proper soft-tissue growth.
Publication Date: 2016-11-01 PubMed ID: 27965496DOI: 10.5005/jp-journals-10024-1949Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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- Journal Article
Summary
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The research article is about the study on the effectiveness of socket preservation technique using equine bone granules and equine collagen matrix, intended to facilitate postextraction healing and tissue regeneration post tooth extraction.
Objective
The main aim of the study revolves around testing the effectiveness of a socket-preservation technique utilizing enzyme-treated equine bone granules (horse bone particles treated with enzymes) along with an equine collagen matrix (a scaffold made from horse collagen) to aid in soft-tissue regeneration and bone grafting.
Methodology
- A patient had one mandibular molar extracted, followed by grafting of the socket with equine bone granules.
- The graft was then overlaid with a double layer of the equine collagen matrix. The surgical site was left partially open to promote healing via a process called ‘Secondary Intention’.
- A month later, an adjacent molar was removed without any accompanying treatment and left to heal similarly.
- At the three-month point post both surgeries, implants were placed and biopsies collected from both sites.
- These biopsies were then subjected to histological processing and evaluation, and a histomorphometric analysis was conducted to measure the percentage of new bone formation.
Results
- Healing took place without complications at both sites and did not result in inflammation or any adverse reactions.
- Secondary intention, or natural healing without wound closure, appeared to occur more quickly at the graft-covered site.
- The biopsy from the grafted site revealed a clear distinction between soft and hard tissues, absent in the biopsy from the untreated site which revealed signs of soft-tissue overgrowth.
- The newly formed bone at the grafted and non-grafted sites showed no significant difference (27.2 ± 7.1 and 29.4 ± 6.2% respectively, p = 0.45).
Conclusion
- The research concluded that the surgical technique, utilizing equine bone granules and equine collagen matrix, facilitated soft-tissue healing post-extraction and simplified the overall management of soft-tissue.
- Moreover, by using a collagen-based matrix to cover a post-extraction grafted site, second intention soft-tissue healing may be expedited and proper soft-tissue growth encouraged.
Cite This Article
APA
Leonida A, Todeschini G, Lomartire G, Cinci L, Pieri L.
(2016).
Socket Preservation using Enzyme-treated Equine Bone Granules and an Equine Collagen Matrix: A Case Report with Histological and Histomorphometrical Assessment.
J Contemp Dent Pract, 17(11), 890-896.
https://doi.org/10.5005/jp-journals-10024-1949 Publication
Researcher Affiliations
- Department of Dental School, University of Milan-Bicocca, Milan, Italy, Phone: +00390258303922, e-mail: leo.doc73@libero.it.
- Department of Dental School, University of Milan-Bicocca, Milan, Italy.
- Department of Dental Clinic, San Gerardo Hospital, Monza, Italy.
- Department of Neuroscience, Psychology, Drug Research and Child Health (NEUROFARBA), University of Florence, Florence Italy.
- Department of Health Sciences, Interdepartmental Forensic Medicine Section, University of Florence, Florence, Italy.
MeSH Terms
- Alveolar Process / pathology
- Alveolar Ridge Augmentation / methods
- Animals
- Bone Regeneration
- Bone Transplantation / methods
- Collagen / drug effects
- Dental Implants, Single-Tooth
- Enzymes / pharmacology
- Female
- Horses
- Humans
- Mandible / surgery
- Middle Aged
- Molar / diagnostic imaging
- Molar / surgery
- Tooth Extraction
- Tooth Socket / diagnostic imaging
- Tooth Socket / pathology
- Tooth Socket / surgery
- Wound Healing
Citations
This article has been cited 2 times.- Di Stefano DA, Orlando F, Ottobelli M, Fiori D, Garagiola U. A comparison between anorganic bone and collagen-preserving bone xenografts for alveolar ridge preservation: systematic review and future perspectives. Maxillofac Plast Reconstr Surg 2022 Jul 12;44(1):24.
- Gallo N, Natali ML, Sannino A, Salvatore L. An Overview of the Use of Equine Collagen as Emerging Material for Biomedical Applications. J Funct Biomater 2020 Nov 1;11(4).
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